Skip to main content
Addgene
Showing: 10441 - 10470 of 148587 results

  5. pLenti-PE2-BSD

    Plasmid
    #161514
    Purpose
    Expression of PE2 in mammalian cells. Can be used to generate stable cell lines by Blasticidin selection
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2020 Sep 21. pii: 10.103
    Insert
    PE2-P2A-BSD
    Use
    Lentiviral
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1α core promoter
    Available since
    Nov. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    161514-plasmid-map-sequence-id-317692

    Enlarge

  22. pPFO*

    Plasmid
    #120400
    Purpose
    Expresses Perfringolysin O cholesterol sensor, has N-terminal His6-tag and three point mutations, A71T, C459A, and Y181A. Y181A prevents PFO's lytic activity at 4°C.
    Depositor
    Arun Radhakrishnan
    Article
    Das et al Proc Natl Acad Sci U S A. 2013 Jun 25;11
    Insert
    Mutant form of Perfringolysin O
    Use
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    Amino acids 1-27 are deleted, starts at amino aci…
    Promoter
    Available since
    Jan. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    120400-plasmid-map-sequence-id-233255

    Enlarge

  23. pODN-mNG-RHOA

    Plasmid
    #183836
    Purpose
    Repair template for the N-terminal tagging of RhoA with mNeonGreen in human cells using CRISPR/Cas9.
    Depositor
    Alisa Piekny
    Article
    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:
    Insert
    RHOA homology arms with mNeonGreen-linker (RHOA Human)
    Use
    CRISPR; Donor template
    Tags
    mNeonGreen-linker
    Expression
    Mammalian
    Mutation
    Homology arms contain point mutations to remove t…
    Promoter
    Available since
    May 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    183836-plasmid-map-sequence-id-362444

    Enlarge

  30. Lamp1-mTurquoise2

    Plasmid
    #98828
    Purpose
    In vivo visualization of lysosomes (can be used for colocalization studies)
    Depositor
    Dorus Gadella
    Article
    Chertkova et al bioRxiv 160374
    Insert
    Lamp1
    Use
    Tags
    mTurquoise2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    98828-plasmid-map-sequence-id-190174

    Enlarge

Showing: 10441 - 10470 of 148587 results