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Plasmid#44186DepositorInsertD998-EGFR-ET1 (EGFR Human)
UseTags3C cleavge site, protein C peptide tag, his6, SBP…ExpressionMammalianMutationEGFR amino acids 1−998PromoterCVMAvailable sinceMay 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEN_hU6miR-G12-E
Plasmid#25757PurposeEntry vector with human U6 promoter driving mouse G alpha 12 miR30-based shRNA.DepositorInsertG alpha 12 miR-shRNA (Gna12 Mouse)
UseEntry vectorTagsExpressionMutationPromoterAvailable sinceJuly 14, 2010AvailabilityAcademic Institutions and Nonprofits only -
pKK-TEV-mVenus
Plasmid#105791PurposeExpression of your protein of interest in fusion with yellow fluorescent protein at the C-terminus (cleavable by TEV). Useful for FRET experiments (PMID: 11753368, 17040988, 22264545).DepositorTypeEmpty backboneUseFlp-in competentTagsTEV-mVenusExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-msGC-NT21
Plasmid#78102Purposeexpresses the N-terminal fragment of manduca sexta soluble guanylyl cyclase subunits alpha and betaDepositorInsertsfragment of alfa subunit of sGC
fragment of beta subunit of sGC
UseTagsHis-tagExpressionBacterialMutationfragment 1-380 of the beta subunit and fragment 2…PromoterT7Available sinceJune 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-msGC-NT13
Plasmid#75087Purposeexpresses the N-terminal fragment of manduca sexta soluble guanylyl cyclase subunits alpha and betaDepositorInsertsfragment of alfa subunit of sGC
fragment of beta subunit of sGC
UseTagsHis-tagExpressionBacterialMutationfragment 1-380 of the beta subunit and fragment 4…PromoterAvailable sinceMay 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKK-mVenus-TEV
Plasmid#105774PurposeExpression of your protein of interest in fusion with yellow fluorescent protein at the N-terminus (cleavable by TEV). Useful for FRET experiments (PMID: 11753368, 17040988, 22264545).DepositorTypeEmpty backboneUseFlp-in competentTagsmVenus-TEVExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR-2B-Nkx2-5 delta HD-G8
Plasmid#98614PurposeGateway entry vector NKX2-5 _HDDepositorInsertNKX2-5 _HD (Nkx2-5 Mouse)
UseEntry vectorTagsExpressionMutationDeletion of the homeodomain. Replaced by 8 glycin…PromoterAvailable sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCR8-CHD5-FRAGMENT
Plasmid#68879PurposeGW entry vector for CHD5DepositorInsertCHD5 (CHD5 Human)
UseGateway entry vectorTagsExpressionMutation331-672aaPromoternoneAvailable sinceNov. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-MCHpr-DREADD (Gq)-mCherry
Plasmid#221036PurposeExpresses Gq coupled DREADD hM3D under the rat pro-melanin-concentrating hormone promoterDepositorInsertDREADD Gq-mCherry
UseAAV; Rat targetingTagsExpressionMammalianMutationPromoterRat MCHAvailable sinceAug. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-FAS-NES-jRGECO1a-WPRE
Plasmid#141236PurposeAAV vector with Ef1a promoter and LoxFAS sites for Cre-Off expression of jRGECO1a (jRGECO1a will not be expressed in mammalian cells that express Cre recombinase)DepositorInsertNES-jRGECO1a
UseAAV and Cre/Lox; Cre-offTags6xHIS tag and nuclear export signalExpressionMutationPromoterhuman elongation factor-1 alpha (EF-1 alpha)Available sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only