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pBAD-deLACCO1

Plasmid

#208018

Purpose

Control biosensor for extracellular L-lactate

Depositor

Robert Campbell

Article

Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

Insert

deLACCO1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD-R-iLACCO1

Plasmid

#208019

Purpose

Biosensor for intracellular L-lactate

Depositor

Robert Campbell

Article

Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

Insert

R-iLACCO1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 30, 2023

Availability

Academic Institutions and Nonprofits only

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GFP Cav2.2 HA pCAGGS

Plasmid

#206054

Purpose

expression of rabbit Cav2.2 calcium channel with a N-terminal GFP tag and an exofacial double HA tag in domain II

Depositor

Annette Dolphin

Article

Macabuag et al J Neurosci. 2015 Oct 28;35(43):14636-52.

Insert

cacna1b (CACNA1B Rabbit)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV/B-actin

Available since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

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GFP Cav2.2 HA pcDNA3

Plasmid

#206053

Purpose

expression of rabbit Cav2.2 calcium channel with a N-terminal GFP tag and an exofacial double HA tag in domain II

Depositor

Annette Dolphin

Article

Macabuag et al J Neurosci. 2015 Oct 28;35(43):14636-52.

Insert

cacna1b (CACNA1B Rabbit)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-IEo

Plasmid

#201992

Purpose

AAV vector mediating inducible expression of codon optimized immediate early gene IE180 of pseudorabies virus

Depositor

Wei Xu

Article

Du et al Cell Rep Methods. 2023 Jun 16;3(6):10050

Insert

Inducible expression of codon optimized immediate early gene IE180 of pseudorabies virus

Use

AAV

Tags

Expression

Mutation

Promoter

Tight TRE

Available since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-CALPAIN-7-mCherry, F98D

Plasmid

#180653

Purpose

Lentiviral expression vector for CALPAIN-7. Used for generating cell lines. F98D mutation. Has C-terminal mCherry tag. Dox-inducible. Internal ID: WISP20-53.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

CALPAIN-7

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

siRNA resistant to: GCACCCAUACCUUUACAUU

Promoter

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCA528 RPS6KL1 MIT, residues 20-140

Plasmid

#180623

Purpose

Bacterial expression for RPS6KL1 MIT domain, residues 20-140. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-32.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

RPS6KL1 (RPS6KL1 Human)

Use

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 20-140

Promoter

T7

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-THTAP-SNX15, residues 263-342

Plasmid

#180624

Purpose

Bacterial expression for SNX15 MIT residues 263-342. Has N-terminal HIS-GST tag. Internal ID:WISP21-130.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

SNX15 (SNX15 Human)

Use

Tags

HIS-GST

Expression

Bacterial

Mutation

Residues 263-342

Promoter

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCA528 SNX15 MIT residues 263-342

Plasmid

#180625

Purpose

Bacterial expression for SNX15 MIT residues 263-342. Has N-terminal HIS-SUMO tag. Internal ID:WISP21-14

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

SNX15 (SNX15 Human)

Use

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 263-342

Promoter

T7

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCA528 SPASTIN , F124D, residues 108-200

Plasmid

#180629

Purpose

Bacterial expression for SPASTIN MIT, residues 108-200. Has F124D mutation. Has N-term HIS-SUMO tag. Used for FP experiments. Internal ID: WISP21-12.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

SPASTIN

Use

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 108-200, F124D

Promoter

T7

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCA528 SPASTIN , L177D, residues 108-200

Plasmid

#180634

Purpose

Bacterial expression for SPASTIN MIT, residues 108-200. Has L177D mutation. Has N-term HIS-SUMO tag. Used for FP experiments. Internal ID: WISP21-13.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

SPASTIN

Use

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 108-200, L177D

Promoter

T7

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCA528 USP8, residues 1-147

Plasmid

#180636

Purpose

Bacterial expression for USP8 MIT domain, residues 1-147. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-86.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

USP8 (USP8 Human)

Use

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 1-147

Promoter

T7

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCA528 USP54, residues 731-878

Plasmid

#180637

Purpose

Bacterial expression for USP54 MIT domain, residues 731-878. Has N-terminal HIS-SUMO tag. Internal ID:WISP20-85

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

USP54 (USP54 Human)

Use

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pET16B VPS4A, residues 1-84

Plasmid

#180638

Purpose

Bacterial expression for VPS4A MIT domain residues 1-84. Has N-terminal HIS-TAG. Internal ID: WISP05-43

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

VPS4A (VPS4A Human)

Use

Tags

His tag

Expression

Bacterial

Mutation

Residues 1-84

Promoter

T7

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCA528 AMSH 1-178

Plasmid

#160050

Purpose

Expresses MIT domain (residues 1-178) of AMSH as a HIS-SUMO fusion protein. Internal ID: WISP18-22.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

AMSH residues 1-178 (STAMBP Human)

Use

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 1-178

Promoter

T7

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCA528 CALPAIN-7 residues 1-165, V18D

Plasmid

#180612

Purpose

Bacterial expression for CALPAIN-7 MIT domains, residues 1-165. Has V18D mutation. Has N-terminal HIS-SUMO tag. Internal ID: WISP21-26.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

CALPAIN-7

Use

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 1-165, V18D mutation

Promoter

T7

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCA528 CALPAIN-7 residues 1-165, F98D

Plasmid

#180613

Purpose

Bacterial expression for CALPAIN-7 MIT domains, residues 1-165. Has F98D mutation. Has N-terminal HIS-SUMO tag. Internal ID: WISP21-27.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

CALPAIN-7

Use

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 1-165, F98D mutation

Promoter

T7

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-THTAP NRBF2 MIT residues 1-111

Plasmid

#180621

Purpose

Bacterial expression for NRBF2 MIT domain, residues 1-111. Has N-terminal HIS-GST. Internal ID: WISP20-88.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

NRBF2 (NRBF2 Human)

Use

Tags

HIS-GST

Expression

Bacterial

Mutation

Residues 1-111

Promoter

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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Control V5-Twist

Plasmid

#201376

Purpose

Expresses Twist for nucleocytoplasmic transport studies

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

Twist (Twist1 Mouse)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pBactin-mOrange2-CI

Plasmid

#196852

Purpose

Expression of red fluorescent reporter mOrange driven by the strong Beta-actin promoter

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

mOrange2

Use

Tags

Expression

Mammalian

Mutation

None (wt)

Promoter

Chicken bactin (plus Chicken bactin intron)

Available since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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