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pBW2611_pCAG-PV1-PYL-L1-iCre-257C-NLS-BGHpA

Plasmid

#108744

Purpose

Expresses aa257 to C-terminus of Cre recombinase fused to PYL CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Cre recombinase fragment aa257 to C-terminus

Use

Cre/Lox and Synthetic Biology

Tags

NLS and PYL

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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QSOX1-Tol2

Plasmid

#113383

Purpose

evaluate FOXA1 DIV motif enhancer activity near 50 kb of QSOX1 gene

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

QSOX1-enhancer (QSOX1 Human)

Use

Tol2 reporter

Tags

Expression

Mutation

Promoter

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only
pBW2542_pCAG-PV1-VCre-N172-L1-GID1-NLS-BGHpA

Plasmid

#108771

Purpose

Expresses N-terminus to aa172 of VCre recombinase fused to GID1 CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

VCre recombinase fragment N-terminus to aa172

Use

Cre/Lox and Synthetic Biology

Tags

GID1,NLS

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBW2562_pCAG-PV1-GAI-L1-VCre-173C-NLS-BGHpA

Plasmid

#108772

Purpose

Expresses aa173 to C-terminus of VCre recombinase fused to GAI CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

VCre recombinase fragment aa173 to C-terminus

Use

Cre/Lox and Synthetic Biology

Tags

GAI and NLS

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-syn-FLEX-synaptoPAC-minWPRE

Plasmid

#153100

Purpose

Cre-conditional expression of synaptoPAC, a fusion of synaptophysin, mScarlet, and bPAC in neurons

Depositor

Dietmar Schmitz

Article

Oldani et al J Neurochem. 2021 Feb;156(3):324-336. do

Inserts

bPAC(WT)
Synaptophysin (Syp Rat)

Use

AAV

Tags

His tag, mScarlet, and myc tag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-FGF1-4X

Plasmid

#120282

Purpose

Produce human recombinant FGF1-4X in DE3 cells

Depositor

Paul Burridge

Article

Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

Insert

Basic fibroblast growth factor 1 mutant

Use

Tags

6x His tag

Expression

Bacterial

Mutation

Codon optimized for E. Coli production

Promoter

T7 Promoter

Available since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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scAAV-Mlc2v-MPRA

Plasmid

#182649

Purpose

Self complementary AAV vector for high-throughput in vivo measurement of enhancer activity by massively parallel reporter assay

Depositor

William Pu

Article

Akerberg et al Nat Commun. 2019 Oct 28;10(1):4907. doi:

Insert

mCherry

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

rat Mlc2v

Available since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

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p2xFLAGhYAP1-WW mutant

Plasmid

#17792

Depositor

Marius Sudol

Article

Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

Insert

Yes-kinase associated protein (YAP1 Human)

Use

Tags

2xFlag

Expression

Mammalian

Mutation

Tryptophan 199 to Alanine; Proline 202 to Alanine

Promoter

Available since

May 9, 2008

Availability

Academic Institutions and Nonprofits only

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pLX304_zeo

Plasmid

#160092

Purpose

Lentiviral vector for mammalian gene expression

Depositor

Rizwan Haq

Article

Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

Type

Empty backbone

Use

Lentiviral; Gateway destination vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pCK005.6

Plasmid

#153025

Purpose

dCas9, MCP-SoxS(R93A/S101A), J106 scRNA

Depositor

Jesse Zalatan

Article

Fontana et al Nat Commun. 2020 Apr 1;11(1):1618. doi:

Insert

dCas9, MCP-SoxS(R93A/S101A), J106 scRNA

Use

CRISPR

Tags

Expression

Bacterial

Mutation

SoxS has R93A and S101A mutations

Promoter

Available since

Aug. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pSP64-hKCNQ1

Plasmid

#53048

Purpose

Expresses alpha subunit for hKCNQ1 channel in Xenopus oocytes

Depositor

Michael Sanguinetti

Article

Sanguinetti et al Nature. 1996 Nov 7;384(6604):80-3.

Insert

Potassium voltage-gated channel subfamily KQT member 1 (KCNQ1 Human)

Use

Xenopus oocyte expression

Tags

Expression

Mutation

Promoter

SP6

Available since

June 9, 2014

Availability

Academic Institutions and Nonprofits only

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pDONR221_eGFP (open)

Plasmid

#160094

Purpose

Expresses eGFP in mammalian cells

Depositor

Rizwan Haq

Article

Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

Insert

eGFP

Use

Gateway entry vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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C terminal Flag Mios pRK5

Plasmid

#46326

Depositor

David Sabatini

Article

Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

Insert

Mios (MIOS Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

July 15, 2013

Availability

Academic Institutions and Nonprofits only

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pLenti-Blast PhLP1-myc-6xHis

Plasmid

#218178

Purpose

Used to make a lenti virus for stable expression of phosducin-like protein 1 in mammalian cells

Depositor

Barry Willardson

Article

Wang et al Mol Cell. 2023 Nov 2;83(21):3852-3868.e6

Insert

Phosducin like protein 1 (PDCL Human)

Use

Lentiviral

Tags

6x His and Myc

Expression

Mammalian

Mutation

Promoter

Available since

April 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCsy_complex

Plasmid

#89232

Purpose

Plasmid contains the genes Csy1, Csy2, Csy3, Csy4, cloned as a polycistronic operon from bacterium Pseudomonas aeruginosa PA14. Csy3 includes an N-terminal 6his tag and TEV protease cleavage site

Depositor

Blake Wiedenheft

Article

Chowdhury et al Cell. 2017 Mar 23;169(1):47-57.e11. doi:

Inserts

Csy1
Csy2
Csy3
Csy4

Use

Tags

6xHis-TEV protease

Expression

Mutation

Promoter

Available since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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plenti6.3/hCx43-stop

Plasmid

#27383

Depositor

Robin Shaw

Article

Smyth et al J Clin Invest. 2010 Jan 4. 120(1):266-79

Insert

Gja1 (GJA1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 20, 2011

Availability

Academic Institutions and Nonprofits only
myc-KIF5A

Plasmid

#127616

Purpose

N-terminal myc-tagged mouse KIF5A

Depositor

Josef Kittler

Article

Twelvetrees et al Neuron. 2010 Jan 14;65(1):53-65. doi: 10

Insert

KIF5A (Kif5a Mouse)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-SARS-CoV-2 Nsp3TMC111A

Plasmid

#204207

Purpose

Mammalian expression of SARS-CoV-2 Nsp3

Depositor

Allan Weissman

Article

Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

Insert

SARS-CoV-2 Nsp3 (Wuhan-Hu-1; https://www.addgene.org/141257/) (ORF1ab )

Use

Tags

FLAGx1

Expression

Mammalian

Mutation

Cys to Ala in active site of PLpro

Promoter

Available since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pGLACTE-PseudoHT51

Plasmid

#99257

Purpose

Contains expanded CGG repeats (98 repeats with 2 AGG interruptions) within the 5'UTR of FMR1. Internal control with synthetic primer sequence for capillary electrophoresis

Depositor

Karen Usdin

Article

Hayward et al J Mol Diagn. 2017 Nov;19(6):828-835. doi

Insert

FMR1 (FMR1 Synthetic, Human)

Use

Tags

Expression

Mutation

Contains synthetic, non-human, primer sequence do…

Promoter

Available since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pLX304_zeo_eGFP

Plasmid

#160095

Purpose

Expresses eGFP in mammalian cells

Depositor

Rizwan Haq

Article

Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

Insert

eGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge