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pTLR-donor-UAS

Plasmid

#119001

Purpose

Repair template for the TLR reporter with 10 UAS binding sites for yeast Gal4

Depositor

Ralf Kuehn

Article

Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

Insert

mVenus

Use

Tags

Expression

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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68Q-ymsfGFP

Plasmid

#128560

Purpose

Httex1 (without proline-rich region) fused to ymsfGFP

Depositor

Patrick Lajoie

Article

Albakri et al F1000Res. 2018 Aug 10;7:1242. doi: 10.12

Insert

68Q-Httex1 (without proline-rich region) fused to ymsfGFP

Use

Tags

ymsfGFP

Expression

Yeast

Mutation

Promoter

GAL1

Available since

Oct. 28, 2019

Availability

Academic Institutions and Nonprofits only

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GGE177

Plasmid

#120766

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Terminator of gene expression for position 1 (T1_Xpr2)

Depositor

Tristan Rossignol

Article

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

T1_Xpr2

Use

Cloning/assembly plasmid

Tags

Expression

Mutation

Promoter

Available since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pYEXC3H

Plasmid

#49026

Purpose

FX cloning Saccharomyces cerevisiae expression vector with GAL1 promoter and C-terminal 3C protease cleavage site and 10x His tag

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Use

Tags

3C protease site (PreScission site) and decaHis (…

Expression

Yeast

Mutation

Promoter

GAL1

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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CUP Snf5 YFP p316

Plasmid

#15597

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

SNF5 (SNF5 Budding Yeast)

Use

Tags

YFP

Expression

Yeast

Mutation

Promoter

Available since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pYEXNHG3

Plasmid

#49025

Purpose

FX cloning Saccharomyces cerevisiae expression vector with GAL1 promoter and N-terminal 10x His tag, GFP and 3C protease cleavage site

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Use

Tags

3C protease site (PreScission site), GFP, and dec…

Expression

Yeast

Mutation

Promoter

GAL1

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pR5-30

Plasmid

#22290

Depositor

Louise Prakash

Article

Pages et al Genetics. 2008 Sep . 180(1):73-82.

Insert

RAD5 (RAD5 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

D681, E682->AA

Promoter

Available since

Nov. 10, 2009

Availability

Academic Institutions and Nonprofits only
Ube2m

Plasmid

#31423

Depositor

Rachel Klevit

Article

Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

Insert

Ubiquitin Conjugating Enzyme (UBE2M Human)

Use

Tags

HA

Expression

Yeast

Mutation

N/A

Promoter

Available since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only
pPTK015-3-yEGFP_Intra

Plasmid

#84974

Purpose

Type 3, Green fluorescent protein

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

Green fluorescent protein

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Non

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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PSUC2-GFPantisense-CYC1t-pRS413

Plasmid

#64404

Purpose

SUC2 regulated expression of an antisense RNAi construct for GFP

Depositor

Claudia Vickers

Article

Williams et al Microb Cell Fact. 2015 Apr 1;14:43. doi:

Insert

GFPantisense

Use

Tags

Expression

Yeast

Mutation

Promoter

SUC2

Available since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

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p2HG/N795

Plasmid

#1284

Depositor

Susan Lindquist

Article

Picard et al Nature 1990 Nov 8;348(6297):166-8.

Insert

GR (Nr3c1 Rat)

Use

Tags

GPD promoter

Expression

Yeast

Mutation

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
GGE009

Plasmid

#120756

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Promoter for gene expression in position 3 (P3_Tef)

Depositor

Tristan Rossignol

Article

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

P3_Tef

Use

Cloning/assembly plasmid

Tags

Expression

Mutation

Promoter

Available since

Nov. 11, 2019

Availability

Academic Institutions and Nonprofits only

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GGE006

Plasmid

#120747

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Promoter for gene expression in position 2 (P2_Tef)

Depositor

Tristan Rossignol

Article

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

P2_Tef

Use

Cloning/assembly plasmid

Tags

Expression

Mutation

Promoter

Available since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pHIS3p:yomWasabi-Tub1+3'UTR::TRP1

Plasmid

#50649

Depositor

Wei-Lih Lee

Article

Markus et al Traffic. 2015 Jul;16(7):773-86. doi: 10.

Insert

yomWasabi-Tub1

Use

Yeast expression

Tags

yomWasabi

Expression

Mutation

Promoter

HIS3p

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pHIS3p:Venus-Tub1+3'UTR::URA3

Plasmid

#50638

Depositor

Wei-Lih Lee

Article

Markus et al Traffic. 2015 Jul;16(7):773-86. doi: 10.

Insert

Venus-Tub1

Use

Yeast expression

Tags

Venus

Expression

Mutation

Promoter

HIS3p

Available since

Jan. 14, 2014

Availability

Academic Institutions and Nonprofits only

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YEplac181-TAF14

Plasmid

#8625

Depositor

Jeff Strathern

Article

Malagon et al Genetics 2004 Mar;166(3):1215-27.

Insert

TAF14 (TAF14 Budding Yeast)

Use

Tags

Expression

Bacterial and Yeast

Mutation

Wild type

Promoter

Available since

Nov. 15, 2005

Availability

Academic Institutions and Nonprofits only

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pPTK019-4-tAOX1

Plasmid

#84988

Purpose

Type 4, tAOX, Alcohol oxidase 1 terminator

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

Alcohol oxidase 1 terminator

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Non

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pSL6 - Brr2 del247 WT

Plasmid

#90256

Purpose

protein expression

Depositor

Christine Guthrie

Article

Ledoux et al J Biol Chem. 2016 Jun 3;291(23):11954-65

Insert

BRR2

Use

Tags

TAP

Expression

Yeast

Mutation

deleted first 247 amino acids

Promoter

Available since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pPS1739

Plasmid

#8922

Depositor

Pamela Silver

Article

Ferrigno et al EMBO J. 1998 Oct 1. 17(19):5606-14.

Insert

HOG1 (HOG1 Budding Yeast)

Use

Tags

GFP

Expression

Yeast

Mutation

Promoter

Available since

Aug. 19, 2005

Availability

Academic Institutions and Nonprofits only
pMST-1312

Plasmid

#118075

Purpose

pTPI1-cexA-tCYC1

Depositor

Michael Sauer

Article

Steiger et al Metab Eng. 2019 Mar;52:224-231. doi: 10.

Insert

pTPI1-cexA-tCYC1

Use

Tags

Expression

Yeast

Mutation

Promoter

pTPI1

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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