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Plasmid#225959PurposeCMV-Intron-Gag-SpyCas9-NLS. Expresses Gag-SpyCas9-NLS for VLP production.DepositorInsertCMV-Intron-Gag-SpyCas9-NLS
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAB1676 REPAIR.t1
Plasmid#176319PurposeCMV-HIVNES-GS-dCas13bt1- (GGS)2-huADAR2dd(E488Q) (REPAIR.t1 expression)DepositorInsertsUseCRISPRTagsHIV NESExpressionMammalianMutationE488Q, only the deaminase domain (aa 276-702 are …PromoterCMVAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
TetO-dCas9-D3A
Plasmid#78254PurposeExpresses dead Cas9 (dCas9) fused to DNMT3A catalytic domain (CD) linked to IRES-mCherry under the control of TetOp and a minimal CMV promoterDepositorInsertDNMT3A CD aa 598-912 (DNMT3A Human)
UseCRISPRTagsFlag epitope tagExpressionMammalianMutationPromoterCMVAvailable sinceAug. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLX302_HOXB13
Plasmid#70089PurposeExpression of human HOXB13 from CMV promoter, puromycin selection markerDepositorInsertHOXB13 (HOXB13 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
ABE8.20-d
Plasmid#136301Purposeexpresses ABE8.20-d in mammalian cellsDepositorInsertABE8.20-d
UseTagsC-terminal BPNLSExpressionMammalianMutationD10A in S. pyogenes Cas9, TadA mutations describe…PromoterCMVAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSico-CAG-v-H-Ras-IRES-Luciferase/EGFP
Plasmid#58959Purposelentiviral expression of v-H-Ras and luciferase/EGFP reporterDepositorInsertsCMV enhancer/chicken beta actin promoter
v-H-Ras
luc2/EGFP fusion gene
UseLentiviralTagsExpressionMammalianMutationPromoterCMV enhancer/chicken beta actinAvailable sinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
TetO-dCas9-mD3A
Plasmid#78255PurposeExpresses dead Cas9 (dCas9) fused to inactive DNMT3A catalytic domain (CD) linked to IRES-mCherry under the control of TetOp and a minimal CMV promoterDepositorInsertDNMT3A CD aa 598-912 (DNMT3A Human)
UseCRISPRTagsFlag epitope tagExpressionMammalianMutationE756APromoterCMVAvailable sinceJune 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-eTLR
Plasmid#214737Purposeencodes the enhanced traffic light reporter (eTLR) flanked by homology arms for integration into the AAVS1 safe harbor locusDepositorInsertAAVS1-eTLR (PPP1R12C Rabbit, Synthetic, CMV, Anguilla japonica, influenza, Human, Bovine, Chicken)
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
GIV-F1685A FLAG
Plasmid#65948PurposeExpression GIV/Girdin F1685A mutation in Mammalian cellDepositorInsertGIV/Girdin (CCDC88A Human)
UseTags3xFlagExpressionMammalianMutationPhenylalanine 1685 to AlaninePromoterCMVAvailable sinceJan. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23 WT 2014 EBOV Delta-Mucin-Like-Domain
Plasmid#86021PurposeEbola Virus (Makona) Glycoprotein in CMV driven mammalian expression vectorDepositorInsertMakona Ebola Virus Glycoprotein
UseTagsExpressionMammalianMutationLacks mucin-like domainPromoterCMV IEAvailable sinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
FLAG-JAK1Gly590Arg
Plasmid#174573PurposeHuman FLAG-tagged JAK1 Gly590Arg mutant expression (N-term. tag) (CMV prom.)DepositorInsertJAK1Gly590Arg
UseTagsFLAGExpressionMammalianMutationGly590ArgPromoterCMVAvailable sinceJan. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23 A82V 2014 EBOV Delta-Mucin-Like-Domain
Plasmid#86022PurposeEbola Virus (Makona) Glycoprotein in CMV driven mammalian expression vectorDepositorInsertMakona Ebola Virus Glycoprotein
UseTagsExpressionMammalianMutationLacks mucin-like domain; Changed alanine 82 to va…PromoterCMV IEAvailable sinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-WDR34
Plasmid#66797PurposeExpression of human WDR34 tagged with GFP under the control of CMVDepositorInsertWDR34 (WDR34 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-LmoCas6-p300
Plasmid#126486PurposeEncodes L.monocytogenes CRISPR Type I-B Cas6 with N-terminal fusion of Flag epitope and NLS and C-terminal fusion of NLS, human p300 HAT core (aa1048-1664), and Flag tag epitope driven by CMV promoterDepositorInsertLmoCas6-p300
UseCRISPRTagsFlag and Flag, NLSExpressionMammalianMutationPromoterCMV and U6Available sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23 A82V/T230A/D637G 2014 EBOV + Mucin-Like Domain
Plasmid#86020PurposeEbola Virus (Makona) Glycoprotein in CMV driven mammalian expression vectorDepositorInsertMakona Ebola Virus Glycoprotein
UseTagsExpressionMammalianMutationChanged alanine 82 to valine, threonine 230 to al…PromoterCMV IEAvailable sinceJan. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-CasE-p300-Flag
Plasmid#106275PurposeEncodes E.coli CRISPR Type I-E CasE with N-terminal fusion of E tag epitope and NLS and C-terminal fusion of NLS, human p300 HAT core (aa1048-1664), and Flag tag epitope driven by CMV promoterDepositorInsertEcoCasE-p300
UseCRISPRTagsE tag, NLS and FlagExpressionMammalianMutationPromoterCMVAvailable sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
Flag-M4-AR-S650D
Plasmid#171246PurposeMammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant: AR-Ser650Asp (AR-S650D)DepositorInsert3xFlag-tagged human androgen receptor Ser650Asp mutant (AR Human)
UseTagsFlagExpressionMammalianMutationAR-Ser650Asp (AR-S650D)PromoterCMVAvailable sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
Flag-M4-AR-S650A
Plasmid#171245PurposeMammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant: AR-Ser650Ala (AR-S650A)DepositorInsert3xFlag-tagged human androgen receptor Ser650Ala mutant (AR Human)
UseTagsFlagExpressionMammalianMutationAR-Ser650Ala (AR-S650A)PromoterCMVAvailable sinceJune 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
Flag-M4-AR-W741C-S81A
Plasmid#171248PurposeMammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant that binds to bicalutamide: AR-Trp741Cys-Ser81Ala (AR-W741C-S81A)DepositorInsert3xFlag-tagged human androgen receptor Trp741Cys-Ser81Ala mutant (AR Human)
UseTagsFlagExpressionMammalianMutationAR-Trp741Cys-Ser81Ala (AR-W741C-S81A)PromoterCMVAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
Flag-M4-AR-W741C-S81D
Plasmid#171249PurposeMammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant that binds to bicalutamide: AR-Trp741Cys-Ser81Asp (AR-W741C-S81D)DepositorInsert3xFlag-tagged human androgen receptor Trp741Cys-Ser81Asp mutant (AR Human)
UseTagsFlagExpressionMammalianMutationAR-Trp741Cys-Ser81Asp (AR-W741C-S81D)PromoterCMVAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only