We narrowed to 12,715 results for: CAR

1. pcDNA3-HA-CUL1 L756A/I757A

   Plasmid

   #19938

   Depositor

   Yue Xiong

   Article

   Furukawa et al Mol Cell Biol. 2000 Nov . 20(21):8185-97

   Insert

   cullin 1 L756A/I757A (CUL1 Human)
   

   Use

   Tags

   HA2

   Expression

   Mammalian

   Mutation

   Mutation of two adjacent hydrophobic residues, L…

   Promoter

   Available since

   April 17, 2009

   Availability

   Academic Institutions and Nonprofits only

2. pMEH60 Lamp2b MS2 HA

   Plasmid

   #168209

   Purpose

   Mammalian expression of lysosome-associated membrane protein 2b fused to the MS2 dimer and C-terminal HA tag

   Depositor

   Joshua Leonard

   Article

   Hung et al J Extracell Vesicles. 2016 May 13;5:3102

   Insert

   Lamp2b (LAMP2 Human)
   

   Use

   Tags

   HA and MS2 dimer

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pY036_ATP1A1_G5

   Plasmid

   #86618

   Purpose

   Expresses the ATP1A1 G5 crRNA in combination with AsCpf1-3xHA to target ATP1A1 intron 4. U6-crRNA(ATP1A1 G5)-CBh-AsCpf1

   Depositor

   Yannick Doyon

   Article

   Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

   Insert

   ATP1A1 G5 crRNA + AsCpf1-3xHA
   

   Use

   CRISPR; Co-selection via hdr using ouabain

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   March 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. XE223 human myc-Chibby-pCS2+MT

   Plasmid

   #16825

   Depositor

   Randall Moon

   Article

   Takemaru et al Nature. 2003 Apr 24. 422(6934):905-9.

   Insert

   myc-Chibby (CBY1 Human)
   

   Use

   Xenopus expression

   Tags

   6-myc

   Expression

   Mutation

   Promoter

   Available since

   March 13, 2008

   Availability

   Academic Institutions and Nonprofits only

5. AB.pCCL.sin.cPPT.U6.miR-146a-Decoy.hPGK.GFP.WPRE

   Plasmid

   #46590

   Depositor

   Brian Brown

   Article

   Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

   Insert

   miR-146a Decoy (MIR146A, Mir146 Mouse, Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available since

   Aug. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pLJC6-Flag CASTOR1 I280A

   Plasmid

   #84497

   Purpose

   Lentiviral expression of CASTOR2 I280A mutant

   Depositor

   David Sabatini

   Article

   Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

   Insert

   CASTOR1 I280A (GATSL3 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   I280A

   Promoter

   sestrin2

   Available since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pEG302_dCAS9-MQ1(C141S,S317A)_g4+g10+g18

   Plasmid

   #172317

   Purpose

   CRISPR dCas9 directly fused to a catalytic inactive version of bacterial DNA methyltransferase MQ1 to target to the FWA locus with three guide RNAs

   Depositor

   Steven Jacobsen

   Article

   Ghoshal et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

   Insert

   g18_U6_g10_U6_g4_U6_UBQ10_Ω_dCas9_MQ1(C141S,S317A)_OCS
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Aug. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBW1310_pCAG-PV1-NTD-ENTRY-L1-PYL-NLS-BGHpA

   Plasmid

   #108838

   Purpose

   Insert protein fragment into MluI/BspEI to fuse to PYL/NLS tags on C-terminus

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   PYL,NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBW1311_pCAG-PV1-ABI-L1-CTD-ENTRY-BGHpA

   Plasmid

   #108839

   Purpose

   Insert protein fragment into KpnI/EcoRI to fuse to ABI tag on N-terminus

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   ABI

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBW1049_pCAG-PV1-NTD-ENTRY-L1-FKBP-NLS-BGHpA

    Plasmid

    #108834

    Purpose

    Insert protein fragment into MluI/BspEI to fuse to FKBP/NLS tags on C-terminus

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    FKBP,NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBW1050_pCAG-PV1-NLS-FRB-L1-CTD-ENTRY-BGHpA

    Plasmid

    #108835

    Purpose

    Insert protein fragment into KpnI/EcoRI to fuse to FRB/NLS tag on N-terminus

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    FRB,NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMXs_FLAG-ODC2

    Plasmid

    #133257

    Purpose

    Express FLAG-tagged ODC2 in mammalian cells

    Depositor

    David Sabatini

    Article

    Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

    Insert

    ODC2 (ODC2 Budding Yeast)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    codon-optimized for expression in human cells

    Promoter

    Available since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMXs_FLAG-NDT2

    Plasmid

    #133255

    Purpose

    Express FLAG-tagged NDT2 in mammalian cells

    Depositor

    David Sabatini

    Article

    Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

    Insert

    NDT2 (YEA6 Budding Yeast)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    codon-optimized for expression in human cells

    Promoter

    Available since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDGB3_alpha1 P35S:SV40-AcrIIA4_CONb:Tnos (GB3344)

    Plasmid

    #160635

    Purpose

    TU for SV40-AcrIIA4 protein Codon optimized for N. benthamiana expression under the regulation of the 35S promoter and Tnos terminator

    Depositor

    Diego Orzaez

    Article

    Calvache et al Plant Biotechnol J. 2022 Feb;20(2):399-4

    Insert

    AcrIIA4
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    35S

    Available since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1/GS-DJ1-E64D-V5

    Plasmid

    #29344

    Depositor

    Mark Cookson

    Article

    Miller et al J Biol Chem. 2003 Sep 19. 278(38):36588-

    Insert

    DJ1 (PARK7 Human)
    

    Use

    Tags

    His6 and V5 epitope

    Expression

    Mammalian

    Mutation

    Glu64Asp

    Promoter

    Available since

    April 20, 2011

    Availability

    Academic Institutions and Nonprofits only

16. Frt-V5-hspB10

    Plasmid

    #63111

    Purpose

    Expression of HSPB10 (small HSP) tagged with V5 in mammalian cells

    Depositor

    Harm Kampinga

    Article

    Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

    Insert

    hspb10 (ODF1 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPN460

    Plasmid

    #137878

    Purpose

    PiggyBac vector for expression of 1x gRNA targeting POGZ for CRISPRa;mRFP-T2A-BlasticidinR

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

    Insert

    POGZ (POGZ Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUPD2 AtU6-26:LbCas12a_DR (GB1443)

    Plasmid

    #160575

    Purpose

    LbCas12a direct repeat sequence fused to the A. thaliana U6-26 promoter used for the assembly of gRNA expression cassettes.

    Depositor

    Diego Orzaez

    Article

    Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

    Insert

    AtU6-26:LbCas12a_DR
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    U6-26

    Available since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. AB.pCCL.sin.cPPT.U6.miR-210-Decoy.hPGK.GFP.WPRE

    Plasmid

    #46599

    Depositor

    Brian Brown

    Article

    Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

    Insert

    miR-210 Decoy (MIR210, Mir210 Mouse, Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pSR40.15

    Plasmid

    #125082

    Purpose

    Expression of ompR(1-137aa)-ccaR(127-234aa) chimera under PLtetO-1, output Promoter PcpcG2-172

    Depositor

    Jeffrey Tabor

    Article

    Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

    Inserts

    ompR(REC)-ccaR(DBD)137
    sfgfp
    tetR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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