-
Plasmid#126135PurposeConstitutive expression vector for Escherichia coli. BglBrick compatible.DepositorInsertGFPuv
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23105Available sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
VR122 pETv2 10His-pG-Tn5 (E54K, L372P)
Plasmid#198467PurposeE. coli protein expression plasmid encoding hyperactive Tn5 transposase fused to protein G, in DH5α cloning strain.DepositorInsert10His-pG-Tn5 (E54K, L372P)
UseTags10His-pGExpressionBacterialMutationE54K, L372PPromoterConsensus φ10 T7 PromoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Tet
Plasmid#138086PurposeEmpty vector with Tet-response promoter for inducible expression of proteins. Can be transduced using Lentiviral particlesDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterminimal CMV promoter with heptamerized tet‐operat…Available sinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJH1579
Plasmid#178904PurposePglr-1::YC3.60 unc-54 3' UTR C.elegans neural expression of Pglr-1 YC3.60DepositorInsertYC3.60
UseTagsExpressionWormMutationSee Depositor Comments BelowPromoterPglr-1Available sinceJan. 6, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBbA13a-RFP
Plasmid#126086PurposeConstitutive expression vector for Escherichia coli. BglBrick compatible.DepositorInsertRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23102Available sinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lenti-entry-puro
Plasmid#85745PurposeLentiviral vector for generating multiple sgRNAs carrying plasmids by Golden Gate Assembly.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pESC-leu2d(empty)
Plasmid#20120DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceFeb. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pDHt/sk-CC
Plasmid#92127PurposeExpression of codon-optimized Cas9 with Pcbh1 promoter in Trichoderma reeseiDepositorInsertCas9
UseCRISPR; Trichoderma reesei expressionTagsExpressionMutationPromoterPcbh1Available sinceNov. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
5xGAL4-TATA-luciferase
Plasmid#46756PurposeLuciferase reporter gene for factors with GAL4 DNA binding domainsDepositorInsertLuciferase
UseLuciferaseTagsExpressionMutationPromoterminimal TATAAvailable sinceAug. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ERK-SPARK
Plasmid#106921PurposeExpress ERK-SPARK, an activity reporter of ERK in mammalian cells.DepositorInsertERK-SPARK
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-EFS-PCP-GFPnls
Plasmid#121938PurposeCRISPR-Sirius plasmidDepositorInsertPCP-GFPnls
UseLentiviralTagssfGFPExpressionMammalianMutationPromoterEFSAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pgRNA-modified
Plasmid#84477Purposeguide RNA expression lentiviral constructDepositorInsertguide RNA
UseLentiviralTagsExpressionMammalianMutationPromotermU6Available sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti-mCherry-CDK4KTR
Plasmid#126680PurposeFluorescent reporter for CDK4/6 activityDepositorInsertRB cterm
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEvol-NnSULT1C1-cysDNCQ
Plasmid#188983Purposebiosynthesize sulfotyrosine in E. coli.DepositorInsertsNnSULT1C1
cysD
cysN
cysC
cysQ
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PKA-SPARK
Plasmid#106920PurposeExpress PKA-SPARK, an activity reporter of protein kinase A in mammalian cells.DepositorInsertPKA-SPARK
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pC0041-RanCas13b crRNA backbone
Plasmid#103852PurposeFor cloning of guide RNAs compatible with RanCas13b. Contains a 3' direct repeat. Clone using BbsI (BpiI). F overhang cacc. R overhang caac.DepositorInsertU6-RanCas13b DR-BbsI-BbsI-polyT
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pC0091
Plasmid#181955PurposeBeta catenin reporter control M51 Super 8x (mutated TCF/LEF binding sites) FOPFlash with Gluc/ClucDepositorInsert7xFOP
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUBC_V5_MS2-mCherry
Plasmid#68420PurposeTransient expression of humanized MS2 (V75E/A81G), fused to mCherry in mammalian cells, under a UBC promoterDepositorInsertMS2
UseSynthetic BiologyTagsV5 and mCherryExpressionMammalianMutationMS2: V75E/A81GPromoterhuman UbCAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
TALED_Right-RNR2-1397N
Plasmid#183897PurposeExpresses TALED_Right-RNR2-1397N in mammalian cellsDepositorInsertTALED for RNR2 mutation
UseTagsExpressionMammalianMutationPromoterpCMVAvailable sinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
TALED_Left-RNR2-1397C-AD
Plasmid#183896PurposeExpresses TALED_Left-RNR2-1397C-AD in mammalian cellsDepositorInsertTALED for RNR2 mutation
UseTagsExpressionMammalianMutationPromoterpCMVAvailable sinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only