Skip to main content
Addgene
Showing: 6861 - 6880 of 37276 results

  2. pET29b-AviTag(K10A)-eGFP-dspB(E184Q)-6xHis

    Plasmid
    #175788
    Purpose
    Plasmid for overexpression of recombinant AviTagged(nonfunctional), His-tagged fusion protein eGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.
    Depositor
    Mark Nitz
    Article
    Eddenden et al ACS Chem Biol. 2020 May 15;15(5):1204-12
    Insert
    Dispersin B
    Use
    Tags
    AviTag, Hexahistidine tag, and eGFP
    Expression
    Bacterial
    Mutation
    E184Q
    Promoter
    T7
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    175788-plasmid-map-sequence-id-345285

    Enlarge

  9. p663-UBC-miniTurbo-V5-LMTK2_IDG-K

    Plasmid
    #170580
    Purpose
    Gateway destination clone of LMTK2 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    miniTurbo-V5-LMTK2 (LMTK2 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    Sept. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    170580-plasmid-map-sequence-id-341636

    Enlarge

  13. pACEBac1-Q22YU3 del C3-2xStrep

    Plasmid
    #170316
    Purpose
    Plasmid for insect cell expression of Tetrahymena thermophila Q22YU3 with a C-terminal truncation (C3-finger). Tagged with 2x Strep-tag II at the C-terminus.
    Depositor
    Andrew Carter
    Article
    Mali et al Science. 2021 Feb 26;371(6532):910-916.
    Insert
    Q22YU3 C-terminal truncation (Delta-C3)
    Use
    Tags
    Strep-Tag II
    Expression
    Insect
    Mutation
    Deletion of C-terminal C3-finger
    Promoter
    polyhedrin
    Available since
    May 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    170316-plasmid-map-sequence-id-333816

    Enlarge

  17. p667-UBC-HIPK3-V5-miniTurbo_IDG-K

    Plasmid
    #158191
    Purpose
    Gateway destination clone of HIPK3 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    HIPK3-V5-miniTurbo (HIPK3 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    V5-miniTurbo
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    Jan. 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    158191-plasmid-map-sequence-id-310258

    Enlarge

  18. pGEX-2T-RNAPII-A2-14-HA

    Plasmid
    #160689
    Purpose
    Expression of GST-RNA Polymerase II C-terminal domain fusion protein. Contains an S-to-A mutation in position 2 of 14 of the consensus heptapeptide repeats shown to be phosphorylated by Cdc2 kinase.
    Depositor
    Jeffry Corden
    Article
    Patturajan et al J Biol Chem. 1998 Feb 20;273(8):4689-94.
    Insert
    RNA polymerase II CTD
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Contains an S-to-A mutation in position 2 of 14 o…
    Promoter
    Available since
    Nov. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    160689-plasmid-map-sequence-id-317780

    Enlarge

Showing: 6861 - 6880 of 37276 results