pET30 W96A-GLTP
(Plasmid
#170734)
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PurposeThe plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 170734 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepET30 Xa/LIC
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameW96A-GLTP
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SpeciesH. sapiens (human)
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MutationW96A
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Entrez GeneGLTP
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Tag
/ Fusion Protein
- His (N terminal on backbone)
Cloning Information
- Cloning method Ligation Independent Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET30 W96A-GLTP was a gift from Rhoderick Brown (Addgene plasmid # 170734 ; http://n2t.net/addgene:170734 ; RRID:Addgene_170734) -
For your References section:
Structural basis for glycosphingolipid transfer specificity. Malinina L, Malakhova ML, Teplov A, Brown RE, Patel DJ. Nature. 2004 Aug 26;430(7003):1048-53. doi: 10.1038/nature02856. 10.1038/nature02856 PubMed 15329726
