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Plasmid#62933PurposeExpression of Cas9-NLS-6xHis in bacterial cellsDepositorInsertCas9-NLS-6xHis
UseTags6xHis tag and NLSExpressionBacterialMutationPromoterT7Available sinceMay 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP_IRES_tdTomato
Plasmid#184046PurposeExpresses EGFP and tdTomato in mammalian cells under control of a CMV promoter by using an IRES sequenceDepositorInsertsEnhanced Green Fluorescent Protein
tdTomato
Internal Ribosome Entry Site
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJRH029
Plasmid#171060PurposePlasmid for expressing Gag fused to spyCas9-3x FLAG-2x SV40 NLS with the SQNYPIVQ HIV-1 cleavage site in betweenDepositorInsertGag-spyCas9
UseCRISPR and LentiviralTags2x SV40 NLS and 3x FLAG tagExpressionMammalianMutationPromoterCAGAvailable sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-MBP-2xNLS-tdTomato
Plasmid#104054PurposeAn AAV genome encoding expression of the nuclear localized fluorescent protein tdTomato from the MBP promoterDepositorInsert2xNLS-tdTomato
UseAAVTagsNLSExpressionMutationPromoterMBPAvailable sinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTS96 pHAGE2 CMVtetO2 GFP-22xGS-SUMOEu-MCS
Plasmid#199349PurposeLentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-SUMOEu-tagged protein in mammalian cells.DepositorTypeEmpty backboneUseLentiviralTagsEGFP-SUMOEuExpressionMammalianMutationWTPromoterAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
Flag Nprl2 pRK5
Plasmid#46333DepositorInsertNprl2 (NPRL2 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceJuly 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
(SIM)5
Plasmid#126947PurposeBacterial expression plasmid for (SIM)5 client to assess SUMO-SIM scaffolds.DepositorInsert5 repeats of SUMO interacting motif (SIM) from PIASx, each separated by (GGS)4 (PIAS2 Human)
UseTagsExpressionBacterialMutationPromoterunknownAvailable sinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pME18s-HA-hFIP200
Plasmid#24303DepositorInsertFIP200 (RB1CC1 Human)
UseTags3xHAExpressionMammalianMutationPromoterAvailable sinceJune 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Barr2 flag
Plasmid#14685DepositorInsertbeta-arrestin 2 (Arrb2 Rat)
UseTagsflagExpressionMammalianMutationPromoterAvailable sinceJune 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
pSLC-255
Plasmid#124292PurposeExpression of vsfGFP-9. This construct improves brightness of sfGFP by fusing a GFP-specific single domain antibody to it that creates a monomeric fluorophore.DepositorInsertvsfGFP-9
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAVS1_Puro_PGK1_3xFLAG_Twin_Strep
Plasmid#68375PurposeDoyon lab Tandem-Affinity Purification Following (i) Nuclease-Driven Gene Addition to the AAVS1 Genomic Safe Harbor Locus and (ii) Endogenous TAP Tagging.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPR and TALEN; ZfnTags3xFLAG_Twin_StrepExpressionMammalianMutationPromoterhuman PGK1Available sinceOct. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAT005
Plasmid#171632Purpose2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA targeting B2M and an EF1-a promoter expressing mNeonGreenDepositorInsertsspyCas9 sgRNA-B2M
mNeon
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1-a and U6Available sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pINDUCER21-puro_Gateway-3xFLAG
Plasmid#172981PurposeGateway Destination vector for doxycycline-inducible expression of ORFDepositorTypeEmpty backboneUseLentiviralTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
Fragment1 with TLS2
Plasmid#196983PurposeGateway-compatible gRNA backbone with TLS2 mobility signal. Template for the addition of target sequences to produce 2x graft-mobile gRNA-TLS2.DepositorInsertIntermediate fragment for assembly of gRNA-TLS2 construct
UseGateway-compatible entry vectorTagsExpressionMutationPromoterAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
myc EDC4 pCMVSport6
Plasmid#86855Purposemammalian expression of EDC4DepositorInsertEDC4 (EDC4 Human)
UseTagsmycExpressionMammalianMutationPromoterCMVAvailable sinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
NC16 pCDNA3.1 FLAG NRF2
Plasmid#36971DepositorInsertNRF2 (NFE2L2 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
sgRNA(MS2) cloning backbone
Plasmid#61424PurposesgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceDec. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/Flag-WTAP
Plasmid#53741PurposeFor expression of WTAP (Wilms tumor 1 associated protein) in human cellsDepositorInsertWilms tumor 1 associated protein (WTAP Human)
UseTagsFLAG tagExpressionMammalianMutationPromoterT7Available sinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV7.1_3xFLAG-WWC2
Plasmid#172995Purposeconstitutive expression of FLAG-tagged WWC2DepositorInsert3xFLAG-WWC2 (WWC2 Human)
UseTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaM-8
Plasmid#172772PurposeBacterial expression of anti-mCherry nanobody LaM-8, with pelB leader and C-term free cysteine and 6xHIS tag.DepositorInsertLaM-8
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only