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Plasmid#187920PurposeExpresses PA subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cellsDepositorInsertpolymerase acidic protein
UseTagsUNTAGGEDExpressionMammalianMutationPromoterCMV PromoterAvailable sinceMay 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PB1-B/BRISBANE/60/2008
Plasmid#187919PurposeExpresses PB1 subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cellsDepositorInsertpolymerase basic protein 1
UseTagsUNTAGGEDExpressionMutationPromoterCMV PromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PB2-B/BRISBANE/60/2008
Plasmid#187917PurposeExpresses PB2 subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cellsDepositorInsertpolymerase basic protein 2
UseTagsUNTAGGEDExpressionMutationPromoterCMV PromoterAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC57-LbNOX
Plasmid#75285PurposeThis plasmid contains human codon optimized sequence of LbNOX which is a water-forming NADH oxidase that can be used to increase NAD+/NADH ratio in mammalian cells.DepositorInsertLbNOX
UseTagsC-terminal FLAG tag with linkerExpressionMutationHuman codon optimizedPromoterAvailable sinceMay 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
FAB(ER-LD)
Plasmid#214424PurposeDetection of ER-lipid droplet contact siteDepositorInsertFRB-V5
UseTagsCFAST-Halo and RspAN-FRB-V5ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PA-3x-FLAG-B/BRISBANE/60/2008
Plasmid#187922PurposeExpresses C terminal 3xFLAG tagged PA subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cellsDepositorInsertpolymerase acidic protein
UseTags3xFLAGExpressionMutationPromoterCMV PromoterAvailable sinceSept. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV SCH9
Plasmid#212708PurposeA SCHEMA evolved AAV variant effective at targeting subventricular NSCsDepositorInsertAAV2 Rep SCH9 Cap
UseAAVTagsExpressionMutationPromoterAvailable sinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
ABE8e(TadA-8e V106W)
Plasmid#138495PurposeA-to-G base editorDepositorInsertecTadA(8e V106W)-nSpCas9
UseTagsExpressionMammalianMutationTadA-8e V106WPromoterAvailable sinceMarch 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAVS1_Puro_Tet3G_3xFLAG_Twin_Strep
Plasmid#92099PurposeDoyon lab Tandem-Affinity Purification Following Nuclease-Driven Gene Addition to the AAVS1 Genomic Safe Harbor Locus. Transgene expression is controlled by an Auto-Regulated Tet-On 3G System.DepositorTypeEmpty backboneUseCRISPR and TALEN; ZfnTagsExpressionMammalianMutationPromoterTet-On 3G BidirectionalAvailable sinceJune 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
FAB(ER-mito)
Plasmid#214427PurposeDetection of ER-mito contact siteDepositorInsertFRB-V5
UseTagsCFAST10-Halo and RspAN-FRB-V5ExpressionMammalianMutationPromoterCMVAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
dCAS9-VP64_GFP
Plasmid#61422PurposeExpresses dCAS9-VP64 activator with 2A GFPDepositorHas ServiceConcentrated Lentiviral PrepInsertdCAS9(D10A,H840A)-VP64_2A_GFP
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A and H840A in Cas9PromoterEF1AAvailable sinceDec. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK2-let-7 WT
Plasmid#78260PurposepsiCHECK2 dual luciferase reporter harboring a fully complementary let-7 target element, cloned into the XhoI/NotI restriction sites, the 3'UTR of the Renilla Luciferase geneDepositorInsertfully complimentary let-7 target site
UseLuciferase; Microrna activityTagsExpressionMammalianMutationPromoterAvailable sinceJune 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSD141B
Plasmid#218139PurposeLentiviral vector expressing evolved degron SD56 fused to eGFP with mCherry control.DepositorInsertSD56-eGFP-IRES2-mCherry
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SpRY-ABE8e
Plasmid#185671PurposeExpresses SpRY-ABE8e in mammalian cellsDepositorInsertecTadA(8e)-nSpRY
UseCRISPRTagsExpressionMammalianMutationSpRY D10APromoterAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-APEX.NES-P2A-EGFP
Plasmid#182824PurposeCre-dependent expression of cytosolic APEXDepositorInsertAPEX.NES-P2A-EGFP
UseAAVTagsEGFP reporter (P2A)ExpressionMutationPromoterEF1aAvailable sinceMay 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLentiCas9-BFP
Plasmid#78545PurposeFusion protein Cas9+BFPDepositorInsertBFP
UseLentiviralTagsExpressionMammalianMutationPromoterFusion protein with Cas9Available sinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMIA22
Plasmid#214448PurposeExpresses BxbI-BP-NLSDepositorInsertBxbI
UseTagsBP-NLSExpressionMammalianMutationPromoterCMVAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBT281
Plasmid#122611Purposeexpress evoAPOBEC1-BE4max in mammalian cellsDepositorInsertevoAPOBEC1-BE4max
UseTagsExpressionMammalianMutationE4K H109N H122L D124N R154H A165S P201S F205SPromoterAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
Cyto-roGFP
Plasmid#49435PurposeExpresses thiol redox-sensitive ratiometric sensor roGFP in cytosol of mammalian cellsDepositorInsertroGFP2
UseAdenoviralTagsExpressionMammalianMutationC48S, Q80R, S147C, and Q204C in Clontech's G…PromoterCMVAvailable sinceDec. 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pU6-Cj-sgRNA
Plasmid#89753PurposeU6 promoter driven expression of Cj-SgRNA cloned with BsmbIDepositorInsertCj-SgRNA
UseSgrna expression under u6 promoterTagsExpressionMutationPromoterU6Available sinceMay 25, 2017AvailabilityAcademic Institutions and Nonprofits only