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Plasmid#19108DepositorInsertProtein DOM34 (DOM34 Budding Yeast)
UseTagsC tagExpressionBacterialMutationF218APromoterAvailable sinceDec. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
Dom34 E261K
Plasmid#19109DepositorInsertProtein DOM34 (DOM34 Budding Yeast)
UseTagsC tagExpressionBacterialMutationE261KPromoterAvailable sinceDec. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
Dom34 Y300A
Plasmid#19110DepositorInsertProtein DOM34 (DOM34 Budding Yeast)
UseTagsC tagExpressionBacterialMutationY300APromoterAvailable sinceDec. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
Dom34 K45A
Plasmid#19111DepositorInsertProtein DOM34 (DOM34 Budding Yeast)
UseTagsC tagExpressionBacterialMutationK45APromoterAvailable sinceDec. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET19b NFRKB 1-101
Plasmid#19787DepositorInsertNFRKB (NFRKB Human)
UseTagsHisExpressionBacterialMutationonly contains amino acids 1-101PromoterAvailable sinceNov. 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
HI.HslU(delI)V.RSET.wt(Nde1).107-GG-244
Plasmid#17859DepositorInsertHaemophilus influenzae heat shock loci U and V
UseTagsExpressionBacterialMutationcoding sequence for residues 108-243 of HslU (the…PromoterAvailable sinceMay 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
pSB700 Cerulean-Weissman scaffold
Plasmid#79377PurposeCas9 gRNA variant to recruit ms2 proteinsDepositorInsertscaffold
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pSB700 Cerulean-Zhang2.0
Plasmid#79378PurposeCas9 gRNA variant to recruit ms2 proteinsDepositorInsertzhang 2.0
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pHWBF06EC
Plasmid#130827PurposeExpression of plant LINC fluorescent fusion protein for cell biologyDepositorInsertSUN2
UseGateway entry vectorTagsmCherry-FLAG-HAExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
SUN2delCC
Plasmid#130836PurposeExpression of plant LINC fluorescent fusion protein for cell biologyDepositorInsertSUN2
UseTagsmCherry-FLAG-HAExpressionPlantMutationDeletion of coiled coil domainPromoterCaMV 35SAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
SUN2delSUNec
Plasmid#130847PurposeExpression of plant LINC fluorescent fusion protein for cell biologyDepositorInsertSUN2
UseGateway entry vectorTagsmCherry-FLAG-HAExpressionMutationDeletion of SUN domainPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
SUN2delCCec
Plasmid#130848PurposeExpression of plant LINC fluorescent fusion protein for cell biologyDepositorInsertSUN2
UseGateway entry vectorTagsmCherry-FLAG-HAExpressionMutationDeletion of coiled coil domainPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pFSW Syt1-C2B R398,399Q IRES GFP
Plasmid#133819PurposeEncodes the C2B domain of synaptotagmin 1 with mutations R398,399Q for viral expressionDepositorInsertSyt1 (Syt1 Rat)
UseLentiviralTagsIRES-GFPExpressionMutationR398,399QPromoterhSyn (human synapsin I promoter)Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pFSW Syt1-C2B K326,327E IRES GFP
Plasmid#133820PurposeEncodes the C2B domain of synaptotagmin 1 with mutations K326,327E for viral expressionDepositorInsertSyt1 (Syt1 Rat)
UseLentiviralTagsIRES-GFPExpressionMutationK326,327EPromoterhSyn (human synapsin I promoter)Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-SauriCas9
Plasmid#135964PurposeExpresses SauriCas9, and cloning backbone for sgRNADepositorTypeEmpty backboneUseAAVTagsExpressionMammalianMutationPromoterAvailable sinceApril 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSD080A
Plasmid#218138PurposeLentiviral vector expressing evolved degron SD40 fused to eGFP with mCherry control.DepositorInsertSD40-eGFP-IRES2-mCherry
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCRISPRia-v2
Plasmid#84832PurposeCRISPRi/a V2 library parental plasmidDepositorInsertssgRNA
puro-T2A-BFP
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermU6Available sinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
eSpCas9(1.1)_No_FLAG_AAVS1_T2
Plasmid#79888PurposeExpresses the AAVS1 T2 gRNA in combination with FLAGless eSpCas9(1.1) to target the AAVS1 "safe harbor" locus.DepositorTypeEmpty backboneUseCRISPRTagsUntagged eSpCas9(1.1)ExpressionMammalianMutationPromoterCBhAvailable sinceJuly 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLuciferase (GB0096)
Plasmid#68201PurposeProvides the CDS of the firefly luciferase protein as a level 0 GoldenBraid partDepositorInsertLuciferase coding region
UseLuciferase and Synthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PA-B/BRISBANE/60/2008
Plasmid#187920PurposeExpresses PA subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cellsDepositorInsertpolymerase acidic protein
UseTagsUNTAGGEDExpressionMammalianMutationPromoterCMV PromoterAvailable sinceMay 9, 2023AvailabilityAcademic Institutions and Nonprofits only