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Plasmid#213512PurposeattB-containing pDEST plasmid, for use in Multisite Gateway reactionDepositorTypeEmpty backboneUseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only
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pJMP3653
Plasmid#222348PurposeExpression vector with PabstBR promoter, kanR marker, and replicates in A. baumannii and E. coli; empty vector plasmid with MCS for cloning - we recommend NcoI/BamHI.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pdCASclos
Plasmid#73640PurposeTranscriptional repression for gene Spo0A (CAC-2011) in Clostridium acetobutylicum ATCC 824DepositorInsertsdCas9
sgRNA to spo0A
UseE.coli-clostridium shuttle vectorTagsExpressionMutationD10A, H840APromoterPj23119 and ptbAvailable sinceJune 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
Fibronectin-human-EDA in pMAX
Plasmid#120402Purposeenables eukaryotic expression of human EDA (or EIIIA) containing fibronectin based on plasma fibronectin sequenceDepositorInsertEDA-fibronectin (FN1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
Px461-Cas9n-Trp53-sgRNA-beta
Plasmid#88847PurposeCRISPR KO of Trp53DepositorInsertTrp53 (Trp53 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-1x
Plasmid#172281PurposeExpressing EGFP mRNA fused with 1 tandem repeat of a 50-base sequenceDepositorInsertEGFP-N1-1x
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
Human Beta-catenin GFP
Plasmid#71367Purposemammalian expression of human Beta-catenin GFPDepositorInsertBeta-catenin (CTNNB1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pD3sE_SC12
Plasmid#219720PurposeDV3 E dimer with fusion loop mutation and increased expressionDepositorInsertDV3 sE SC12
UseTags8xHis and human serum albumin (HSA)ExpressionMammalianMutationG106D, A257W, T260R, F277W, A278P, G29K, T33V, A3…PromoterAvailable sinceMay 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD4sE_SC12
Plasmid#219721PurposeDV4 stable E dimer with fusion loop mutation and increased expressionDepositorInsertDV4 sE SC12
UseTags8xHis and human serum albumin (HSA)ExpressionMammalianMutationG106D, A259W, S262R, F279W, A280P, G29K, T33V, A2…PromoterAvailable sinceMay 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD1sE_SC12
Plasmid#219718PurposeDV1 E dimer with fusion loop mutation and increased expressionDepositorInsertDV1 sE SC12
UseTags8xHis and human serum albumin (HSA)ExpressionMammalianMutationG106D, A259W, T262R, F279W, A280P, S29K, T33V, A2…PromoterAvailable sinceMay 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBR322-Pdp1_NTD-Cas9
Plasmid#198275PurposeFor loading SpCas9 into PVCs; also contains PVC regulatory genesDepositorInsertPdp1_NTD-Cas9
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
phCMV-coGALVenv-C4070A
Plasmid#172217PurposeExpresses the envelope protein of Gibbon Ape Leukemia Virus (GALV) for pseudotyping of retroviral or lentiviral vectorsDepositorInsertEnvelope protein of Gibbon Ape Leukemia Virus (codon-optimized version)
UseTagsExpressionMammalianMutationCarries C-terminus of amphotropic MLV strain 4070…PromoterCMV immediate early promoterAvailable sinceAug. 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
JAK3 (JAK3A-c023)
Plasmid#98229PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertJAK3A (JAK3 Human)
UseBaculovirusTagsHis-6-TEVExpressionMutationpfam00069:Pkinase 822-1091PromoterpolyhedrinAvailable sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHTC-HaloTag_DDB1:1-1140
Plasmid#211007PurposeMammalian expression of full-length human DDB1. C-terminal HaloTag.DepositorInsertDDB1:M1-H1140 (DDB1 Human)
UseTagsHaloTagExpressionMammalianMutationwild typePromoterCMVAvailable sinceFeb. 21, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
CMV-Brainbow-2.1 R
Plasmid#18723DepositorInserthrGFPII-NLS ; EYFP ; tdimer2 ; M-mCerulean
UseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceAug. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-DIO-mTurquoise2
Plasmid#99115PurposeAn AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein mTurquoise2DepositorInsertDIO-mTurquoise2
UseAAVTagsExpressionMammalianMutationPromoterTREAvailable sinceAug. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.3 LRP5
Plasmid#115907PurposeExpresses human LRP5 in mammalian cells.DepositorInsertLRP5 (LRP5 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
Fibronectin-human-plasma in pMAX
Plasmid#120401Purposeenables eukaryotic expression of human plasma fibronectinDepositorInsertFibronectin (FN1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pmRFP-LC3
Plasmid#21075PurposeMammalian expression of rat LC3 fused to mRFPDepositorInsertmicrotubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
UseTagsmRFPExpressionMammalianMutationFirst ATG is not included.PromoterAvailable sinceMay 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET28b-6His-AcpS
Plasmid#145376PurposeExpression of E. coli AcpSDepositorInsertAcpS (acpP E. coli)
UseTags6-HisExpressionBacterialMutationPromoterAvailable sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits