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Plasmid#69922PurposeExpresses mutant myc-RNF114 in mammalian cellsDepositorInsertRNF114 (RNF114 Human)
UseTagsExpressionMammalianMutationC29A, C32APromoterCMVAvailable sinceFeb. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti.puro.MCV.ER.RAZ.2
Plasmid#114382PurposeThis lentiviral plasmid expresses MCV tumor T antigens (truncated LT and small T) using the viral NCCR (non-coding control region). The sequence was amplifeied from MCV.R17a and truncated LT=428 aa.DepositorInsertMCV NCCR and tumor T antigen regions
UseLentiviralTagsExpressionMammalianMutationPromoterMCV NCCR (viral own promoter)Available sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEasyG3_nat
Plasmid#184918PurposeTemplate to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_nat recombines in vivo with a PCR product from pEasyG3_mic.DepositorInsertgRNA scaffold
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEasyG3_mic
Plasmid#184920PurposeTemplate to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_mic recombines in vivo with a PCR product from pEasyG3_zeo/nat/hph.DepositorInsertgRNA scaffold
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-TWIN-STREP-FLAG-NPRL3
Plasmid#164984PurposeNPRL3 expressing vector for mammalian cellsDepositorInsertNPRL3 (NPRL3 Human)
UseTags2xSTREP FLAGExpressionMammalianMutationPromoterCAGAvailable sinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR-SUMO1
Plasmid#114388PurposepDONR vector with SUMO1 geneDepositorInsertSUMO1 (SUMO1 Human)
UseTagsExpressionMammalianMutationQ29H compared with NCBI reference NP_003343.1PromoterAvailable sinceAug. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
FCK-ArchT-GFP
Plasmid#31177PurposeLentiviral expression of ArchT-GFP for neuronal inhibitionDepositorInsertArchT-GFP
UseLentiviralTagsGFPExpressionMammalianMutationPromoterCaMKIIAvailable sinceSept. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
SF-U7snRNA-CypA-E4
Plasmid#190695PurposeControl vector expressing a U7-based antisense oligonucleotide targeting CypA exon 4DepositorInsertU7 snRNA antisense CypA Exon 4
UseLentiviralTagsExpressionMammalianMutationPromoterU7 promoterAvailable sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAT-PB-CG2APtk
Plasmid#86003Purposehomology donor for AAT locus with piggyBac-flanked eGFP_T2A_puroTK cassetteDepositorInserteGFP_T2A_puro_dTK
UsePiggybac exciseableTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCI-Neo-gp78/JM20
Plasmid#13303DepositorInsertgp78 (AMFR Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
p164 Dync1i1.B
Plasmid#26435DepositorInsertcytoplasmic dynein 1 intermediate chain 1 isoform B (Dync1i1 Mouse)
UseSubcloning and sequencingTagsExpressionMutationN/APromoterAvailable sinceMarch 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
mCherry-H2B-6
Plasmid#55056PurposeLocalization: Nucleus/Histones, Excitation: 587, Emission: 610DepositorInsertH2B (H2BC11 Human)
UseTagsmCherryExpressionMammalianMutationD26G and V119I in H2BPromoterCMVAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHBS1552 H14-SUMO-TDP43 FYW-S-TEV-mCherry
Plasmid#133322PurposeBacterial expression of TDP43 with FYW-S mutant CTDDepositorInsertTARDBP mutant (TARDBP Human)
UseTagsExpressionBacterialMutationAll FYW in the TDP43 IDRs mutated to SPromoterAvailable sinceNov. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Flag HBx (G124L, I127A)
Plasmid#42598DepositorInsertHepatitis B virus protein X (G124L, I127A)
UseTagsFlagExpressionMammalianMutationG124L I127APromoterAvailable sinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRN3P-HA-Suv4-20h1
Plasmid#86689PurposeFor transcription of of Suv4-20h1 mRNA preceded by an HA-tag in 5'DepositorInserthistone-lysine N-methyltransferase KMT5B (Kmt5b Mouse)
UseTagsHA-tagExpressionMutationPromoterT3Available sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
Cav2.2e[a10, 18a, Δ24a, 31a, 37b, 46]
Plasmid#26568DepositorInsertcalcium channel alpha-1B subunit (Cacna1b Rat)
UseTags6XHis and V5ExpressionMammalianMutationcontains exons a10, 18a, 31a, 37b, and 46. Del…PromoterAvailable sinceNov. 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
pSVE Grb2 myc 49L/203R
Plasmid#26087DepositorInsertGrb2 49L/203R (GRB2 Human)
UseTagsmycExpressionMammalianMutation49L/203RPromoterAvailable sinceSept. 23, 2010AvailabilityAcademic Institutions and Nonprofits only -
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pBabe-TetCMV-puro-mVenus-PA-Rac1
Plasmid#22008PurposeMammalian expression of photoactivatable Rac1DepositorInsertPA-Rac1 (RAC1 Avena sativa (oat), Human)
UseRetroviralTagsmVenusExpressionMammalianMutationLOV(Leu404-Leu546), Rac1 starts at I4 and contain…PromoterAvailable sinceSept. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
pXPR_BRD003 sgKEAP1-2
Plasmid#74986PurposesgKEAP1-2, puromycin selectionDepositorInsertKEAP1
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only