Addgene

Showing: 3391 - 3400 of 9036 results

Results per page:

Sort results by:

pPB-PGK-hKLF4

Plasmid

#60435

Purpose

Over-expression of human KLF4

Depositor

Austin Smith

Article

Takashima et al Cell. 2014 Sep 11;158(6):1254-69. doi: 1

Insert

KLF4 (KLF4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 16, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pEN_TT 3xFLAG-KPNA2

Plasmid

#192306

Purpose

Gateway entry vector for an inducible 3xFLAG-KPNA2

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

KPNA2 (KPNA2 Human)

Use

Gateway entry vector

Tags

3xFLAG

Expression

Mutation

Promoter

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pKK-TEV-3XFLAG

Plasmid

#105784

Purpose

Expression of your protein of interest in fusion with 3xFLAG at the C-terminus. The tag is cleavable by TEV protease.

Depositor

Andrzej Dziembowski

Article

Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

Type

Empty backbone

Use

Flp-in competent

Tags

TEV-3XFLAG

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ269E2

Plasmid

#164721

Purpose

Gateway entry clone (attL1 & attR5) A3A/Y130F-CBE_V04 for C-T base editing

Depositor

Yiping Qi

Article

Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

Insert

A3A(Y130F)-zCas9(D10A)-UGI-T2A-MS2-UGI

Use

CRISPR; Gateway compatible a3a/y130f-cbe_v04 entr…

Tags

Expression

Plant

Mutation

Promoter

Available since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR1A mKate2-hu_ncOGT D554N

Plasmid

#154283

Purpose

Entry vector encoding mKate2-2xFLAG-OGT (O-GlcNAc Transferase; D554N variant, no Ser/Thr glycosylation)

Depositor

Suzanne Walker

Article

Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

Insert

O-GlcNAc Transferase (OGT Human)

Use

Gateway entry vector

Tags

2x FLAG and mKate2

Expression

Mutation

D554N mutation (Ser/Thr Glycosylation Impaired)

Promoter

Available since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
LentiGuide-Puro-IFITM3g1 (BB15)

Plasmid

#139460

Purpose

Lentiviral vector with gRNA targeting IFITM3; includes puromycin selectable marker

Depositor

Caroline Goujon

Article

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

IFITM3-targeting sgRNA inserted; resistance gene: puroR (IFITM3 Synthetic)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a / U6

Available since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
SLK_HUMAN_D0

Plasmid

#79733

Purpose

This plasmid encodes the kinase domain of SLK. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

Markus Seeliger, Nicholas Levinson, John Chodera

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

SLK (SLK Human)

Use

Tags

His10-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
MAC-TMPRSS2

Plasmid

#158385

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

TMPRSS2 (TMPRSS2 Human)

Use

Tags

MAC-tag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 26, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pDONR221-ARL8B

Plasmid

#67334

Purpose

hArl8b with stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL8B (ARL8B Human)

Use

Gateway entry vector

Tags

Expression

Bacterial

Mutation

Promoter

None

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pTRE-Tight-FLAG-CYLD-C601A

Plasmid

#60029

Purpose

CYLD (C601A) catalytic dead under control of Tet promoter with N-terminal FLAG tag

Depositor

Stephen Elledge

Article

Stegmeier et al Proc Natl Acad Sci U S A. 2007 May 22. 1

Insert

Cylindromatosis (CYLD Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Cysteine 601 changed to Alanine

Promoter

TRE

Available since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only