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qTAG-C-miRFP670nano3-Puro

Plasmid

#207724

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a miRFP670nano3-2A-Puro cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pLenti-Puro-sgSNRK

Plasmid

#138694

Purpose

Expresses a human SNRK-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgSNRK human (SNRK Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV:Flag-NLuc_SV40:PuroR

Plasmid

#183045

Purpose

Expresses FLAG-tagged NanoLuciferase (NLuc-only) in mammalian cells.

Depositor

Antonio Amelio

Article

Yao et al Nat Methods. 2022 Jul;19(7):893-898. doi

Insert

NanoLuciferase

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-entry-puro

Plasmid

#85745

Purpose

Lentiviral vector for generating multiple sgRNAs carrying plasmids by Golden Gate Assembly.

Depositor

Qin Yan

Article

Cao et al Nucleic Acids Res. 2016 Nov 2;44(19):e14

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pLCE-P2A-Puro

Plasmid

#123318

Purpose

Lentiviral vector for expression of eGFP-P2A-Puro cassette from a CMV promoter

Depositor

Eva Gottwein

Article

Patil et al Blood. 2018 Aug 9;132(6):577-586. doi: 1

Insert

eGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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pQFlag-USP7 WT puroR

Plasmid

#46751

Purpose

Retroviral vector that expresses wild type Flag-tagged human USP7

Depositor

Gordon Peters, Goedele Maertens

Article

Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

Insert

USP7 (USP7 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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Construct 32 - NFKBRp_mKate2_2xNLS_p2a-puroR

Plasmid

#82024

Purpose

Lentiviral construct for building stable cell lines expressing mKate2 in the presence of TNFa via puromycin selection

Depositor

Timothy Lu

Article

Perli et al Science. 2016 Aug 18. pii: aag0511.

Insert

mKate2-2xNLS

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pLV-Puro_hCOL4A4-3FLAG

Plasmid

#198101

Purpose

lentiviral expression of hCOL4A4 in mammalian cells

Depositor

Jeffrey Miner

Insert

COL4A4 (COL4A4 Human)

Use

Lentiviral

Tags

3FLAG

Expression

Mutation

Promoter

EF1a

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pLEX307-TMPRSS2-puro

Plasmid

#158457

Purpose

Constitutive lentiviral expression of human TMPRSS2.

Depositor

Alejandro Chavez, Sho Iketani

Insert

TMPRSS2 (TMPRSS2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3puro-mGFP-TDRD3

Plasmid

#225469

Purpose

Express mEGFP-fusion protein of TDRD3

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

TDRD3 (TDRD3 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro-eGFP-hcGAS

Plasmid

#108674

Purpose

For production of retrovirus and transduction of mammalian cells, allowing expression of N-terminally eGFP-tagged human cGAS

Depositor

Martin Reijns, Andrew Jackson

Article

Mackenzie et al Nature. 2017 Aug 24;548(7668):461-465. d

Insert

eGFP-cGAS (CGAS Human)

Use

Retroviral

Tags

Expression

Mutation

Promoter

LTR

Available since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pBabe-Puro-MEK-DD

Plasmid

#15268

Depositor

William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

Map2k1 (Mapk1 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

S218D S222D

Promoter

Available since

July 12, 2007

Availability

Academic Institutions and Nonprofits only

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pUltra-puro-RTTA3

Plasmid

#58750

Purpose

silence doxycyclin inducible pUltra-dox

Depositor

Yildirim Dogan, Kitai Kim

Insert

Reverse TET-Transactivator 3

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

loxP5171

Promoter

Ubiquitin C promoter

Available since

Oct. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pLKO.1 puro shTEAD1/3/4-1

Plasmid

#193672

Purpose

Constitutive lentiviral expression of TEAD shRNA

Depositor

William Hahn

Article

Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

Insert

TEAD

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pTet-O-Nd1-puro

Plasmid

#52052

Purpose

Expresses Nd1 and puromycin resistent gene under control of TetON promoter

Depositor

Marius Wernig

Article

Zhang et al Neuron. 2013 Jun 5;78(5):785-98. doi: 10

Insert

Neurod1 (Neurod1 Mouse)

Use

Lentiviral

Tags

T2A-and puromycin resistant cassette

Expression

Mammalian

Mutation

Promoter

tet-on

Available since

April 11, 2014

Availability

Academic Institutions and Nonprofits only

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pMXspuro-mCherry-NBR1

Plasmid

#74242

Purpose

Retroviral expression of mCherry-NBR1

Depositor

Jayanta Debnath

Article

Kenific et al J Cell Biol. 2016 Feb 29;212(5):577-590.

Insert

neighbor of BRCA1 gene 1 (NBR1 Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

April 25, 2016

Availability

Academic Institutions and Nonprofits only
pLenti-puro/RAF1

Plasmid

#131725

Purpose

Mammalian Expression of RAF1

Depositor

Jacques De Grève

Article

Noeparast et al Oncogene. 2019 Aug;38(31):5933-5941. doi

Insert

RAF1 (RAF1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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iF59 PB-TO-oNgn2-puro

Plasmid

#204734

Purpose

Improved Dox-inducible neurogenin-2; Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression. oNGN2 is a Phospho-mutant which avoids inhibition by cdk kinase.

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Optimized neurogenin-2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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mc2 puro EF1a

Plasmid

#206221

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Brett Stringer, Simon Conn

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti4-Puro-H19

Plasmid

#200835

Purpose

Lentiviral vector expressing human long non-coding RNA H19

Depositor

Reinier Boon

Article

Hofmann et al Cardiovasc Res. 2019 Jan 1;115(1):230-24

Insert

Human long non-coding RNA H19

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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