Addgene

We narrowed to 5,192 results for: pCas

Showing: 661 - 680 of 5192 results

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pCas9CR4-VQR

Plasmid

#120224

Purpose

pCas9CR4 with VQR mutations for changing PAM site specificity to NGAN or NGNG from Kleinstiver et al. 2015

Depositor

Christopher Reisch

Insert

SpCas9-VQR

Use

Synthetic Biology

Tags

ssrA

Expression

Mutation

Mutations D1135V/R1335V/T1337R

Promoter

pTet

Available since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pMC-6-AtSpCas9-9

Plasmid

#197778

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

AtSpCas9

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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SpCas9 TadCBEe

Plasmid

#193836

Purpose

Express TadCBEe (with SpCas9) in mammalian cells

Depositor

David Liu

Article

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Insert

TadA-CDe-SpCas9D10A-2xUGI

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-mWasabi-ER

Plasmid

#164165

Purpose

Expresses mWasabi fused with an ER-targeting sequence of calreticulin and the ER retrieval sequence, KDEL, under the control of CAG promoter

Depositor

Isei Tanida

Article

Tanida et al Sci Rep. 2020 Dec 14;10(1):21871. doi: 1

Insert

mWasabi

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Feb. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-mTurbo-G

Plasmid

#187176

Purpose

Express miniTurbo under the control of CAG promoter

Depositor

Isei Tanida

Article

Sanada et al Sci Rep. 2022 Jul 1;12(1):11130. doi: 10

Insert

miniTurbo (BirA mutant)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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pCaSpeR-act5cB-QF#7

Plasmid

#46125

Depositor

Christopher Potter

Article

Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

QF#7

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 13, 2013

Availability

Academic Institutions and Nonprofits only

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2X_pX458_pSpCas9(BB)-2A-GFP_NANOG_bKO

Plasmid

#172224

Purpose

Cas9-2A-GFP expression vector bearing two sgRNAs targeting the telomeric human NANOG CTCF-boundary.

Depositor

Alexander Meissner

Article

Wu et al Nat Commun. 2021 Aug 12;12(1):4897. doi:

Insert

sgRNA

Use

CRISPR

Tags

3XFLAG and GFP

Expression

Mammalian

Mutation

Promoter

Cbh

Available since

Sept. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCAGGS-Hoxc6

Plasmid

#45553

Depositor

Jeremy Dasen

Article

Lacombe et al PLoS Genet. 2013 Jan;9(1):e1003184. doi:

Insert

Hoxc6 (Hoxc6 Mouse)

Use

Tags

Expression

Mutation

Promoter

CMV / b-actin

Available since

Aug. 5, 2013

Availability

Academic Institutions and Nonprofits only
pCAR-IF reporter

Plasmid

#16628

Depositor

Bert Vogelstein

Article

Nicolaides et al Mol Cell Biol. 1998 Mar . 18(3):1635-41.

Insert

CAR-IF

Use

Tags

beta-galactosidase

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pCAGGS-synPCB2.0

Plasmid

#139477

Purpose

Expression vector for phycocyanobilin (PCB) synthesis in mammalian cells.

Depositor

Kazuhiro Aoki

Article

Uda et al ACS Chem Biol. 2020 Nov 20;15(11):2896-2

Insert

MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Fnr-Fd-T7

Use

Tags

Expression

Mammalian

Mutation

V413L

Promoter

CAG promoter

Available since

April 16, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-F-API5

Plasmid

#157662

Purpose

Expresses API5 in mammalian cell

Depositor

Byung Il Lee

Article

Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

Insert

Apoptosis inhibitor 5 (API5 Human)

Use

Tags

FLAG tag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-Bmp15

Plasmid

#163594

Purpose

Expresses BMP-15 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmp15 (Bmp15 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pCasper4-QF#7-hsp70

Plasmid

#46135

Depositor

Christopher Potter

Article

Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

QF#7

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pCAT.011

Plasmid

#119555

Purpose

Self replication Level T acceptor vector with LacZ compatible blue/white screening. Modified SEVA vector, RK2 ori vector.

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-p21_S146A

Plasmid

#40205

Depositor

Philipp Mergenthaler

Article

Mergenthaler et al J Cereb Blood Flow Metab. 2013 Mar;33(3)

Insert

p21 (CDKN1A Human)

Use

Tags

Expression

Mammalian

Mutation

S146A

Promoter

CAG

Available since

March 7, 2013

Availability

Academic Institutions and Nonprofits only

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pCAndy-NBioTAP

Plasmid

#53571

Purpose

Plasmid vector carrying N-Bio-1xproteinA tag

Depositor

Mitzi Kuroda

Article

Alekseyenko et al Proc Natl Acad Sci U S A. 2014 Feb 18;11

Type

Empty backbone

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Aug. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pCAndy-CBioTAP

Plasmid

#53570

Purpose

Plasmid vector carrying C-Bio-1xproteinA tag

Depositor

Mitzi Kuroda

Article

Alekseyenko et al Proc Natl Acad Sci U S A. 2014 Feb 18;11

Type

Empty backbone

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Aug. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pSW177 - pCASP1

Plasmid

#115986

Purpose

entry vector for GreenGate cloning method, promoter

Depositor

Alexis Maizel

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

pCASP1

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-p21_S146D

Plasmid

#40206

Depositor

Philipp Mergenthaler

Article

Mergenthaler et al J Cereb Blood Flow Metab. 2013 Mar;33(3)

Insert

p21 (CDKN1A Human)

Use

Tags

Expression

Mammalian

Mutation

S146D

Promoter

CAG

Available since

March 7, 2013

Availability

Academic Institutions and Nonprofits only

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pCAG-OSF-PP

Plasmid

#154247

Purpose

backbone with PreScission protease-cleavable N-terminal OneStrepFLAG tag

Depositor

Wesley Sundquist

Article

Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

Type

Empty backbone

Use

Tags

FLAG and Strep-Tag II

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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