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Plasmid#205298PurposeIntegrating plasmid for ORBIT in E. coli. Orthogonal attP site (#3).DepositorArticleTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 8, 2023AvailabilityAcademic Institutions and Nonprofits only
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pInt_attP1_chlorR
Plasmid#205295PurposeIntegrating plasmid for ORBIT in E. coli. Standard attP site (#1).DepositorArticleTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLIX403-EBFP2-ccdB-G418
Plasmid#158556PurposeSet of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusionsDepositorTypeEmpty backboneUseLentiviralTagsEBFP2ExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLIX403-EYFP-ccdB-G418
Plasmid#158550PurposeSet of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusionsDepositorTypeEmpty backboneUseLentiviralTagsEYFPExpressionMammalianMutationPromoterAvailable sinceAug. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pxCas9CR4
Plasmid#111656PurposePCas9CR4 with the xCas9 mutations allowing NG PAM sitesDepositorInsertTetR and xCas9
UseSynthetic BiologyTagsssrAExpressionBacterialMutationxCas9 mutationsPromoterAvailable sinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pnYC-NpM-HsYTHDC2_36-1430_AD
Plasmid#148487PurposeBacterial Expression of HsYTHDC2_36-1430DepositorInsertHsYTHDC2_36-1430 (YTHDC2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pACYCduet-mxLOX1-EH
Plasmid#104978PurposeBiosynthesis of oxylipins by microbial enzymesDepositorUseTagsExpressionBacterialMutationPromoterT7promoterAvailable sinceFeb. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBCSK_HIS3_II
Plasmid#109059Purposeyeast shuttle vector with deletable CEN6-ARSH4 cassette. For teaching purposes.DepositorInsertHIS3 (HIS3 Budding Yeast, Zygosaccharomyces rouxii)
UseCre/LoxTagsExpressionBacterial and YeastMutationPromoterHIS3Available sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBCSK_HIS3_I
Plasmid#109058Purposeyeast shuttle vector with deletable CEN6-ARSH4 cassette. For teaching purposes.DepositorInsertHIS3 (HIS3 Budding Yeast, Zygosaccharomyces rouxii)
UseCre/LoxTagsExpressionBacterial and YeastMutationPromoterHIS3Available sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pnYC-HsNot1_1-682_AF
Plasmid#148670PurposeBacterial Expression of HsNot1_1-682DepositorInsertHsNot1_1-682 (CNOT1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnYC-NpM-DmNot10_337-635_AF
Plasmid#148671PurposeBacterial Expression of HsNot10_337-635DepositorInsertHsNot10_337-635 (CNOT10 Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnYC-NpM-HsYTHDC2_36-1068_AD
Plasmid#148486PurposeBacterial Expression of HsYTHDC2_36-1068DepositorInsertHsYTHDC2_36-1068 (YTHDC2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnYC-NpM-HsYTHDC2_157-1068_AD
Plasmid#148488PurposeBacterial Expression of HsYTHDC2_157-1068DepositorInsertHsYTHDC2_157-1068 (YTHDC2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnYC-NpM-HsYTHDC2_1069-1430_AD
Plasmid#148489PurposeBacterial Expression of HsYTHDC2_1069-1430DepositorInsertHsYTHDC2_1069-1430 (YTHDC2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBCSK_LEU2_II
Plasmid#109061Purposeyeast shuttle vector for insertion into yeast chromosomes in pBCSK backbone. LoxP sites downstream of LEU2. For teaching purposes.DepositorInsertLEU2 (LEU2 Budding Yeast, Zygosaccharomyces rouxii)
UseCre/LoxTagsExpressionBacterial and YeastMutationPromoterLEU2Available sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCASCADE-udhA-zwf
Plasmid#87153PurposeudhA-zwf targeting guide RNA E. coli , Low Phosphate InductionDepositorInsertudhA-zwf guide array
UseTagsExpressionBacterialMutationPromoterE . coli ugpB gene promoter, low phosphate induct…Available sinceMarch 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEcKdtA
Plasmid#36268DepositorInsertE. coli KdtA
UseTagsExpressionMutationPromoteraraCAvailable sinceJuly 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHiKdtA
Plasmid#36269DepositorInsertH. influenzae KdtA
UseTagsExpressionMutationPromoteraraCAvailable sinceJune 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
Ma-pREP
Plasmid#197571PurposePositive selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses TAG-codon interrupted chloramphenicol resistance cassette and M. alvus Pyl-tRNA(6). p15a oriDepositorInsertsChloramphenicol resistance gene - 111TAG
T7 polymerase -TAG
GFPuv
M. alvus Pyl-tRNA(6)
UseTagsExpressionBacterialMutation111TAG and 8TAG/112TAGPromoterAraC, T7, cat promoter (constitutive), and lpp (c…Available sinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
PL-5LTR-GW-A
Plasmid#138480PurposeFor expression of ribozyme-flanked sgRNA after the Gateway (GW) LR cloning.DepositorInsertGateway attR1/R2 cassette with chloramphenicol resistance and ccdB genes
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only