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Addgene
Showing: 661 - 680 of 868 results

  2. pSpG-PPE

    Plasmid
    #170130
    Purpose
    For plant prime editing in wheat plants or monocotyledons protoplasts
    Depositor
    Caixia Gao
    Article
    Lin et al Nat Biotechnol. 2021 Mar 25. pii: 10.103
    Insert
    nCas9-SpG(H840A)-M-MLV
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    H840A, D1135L, S1136W, G1218K, E1219Q, R1335Q, T1…
    Promoter
    maize Ubiquitin-1
    Availability
    Academic Institutions and Nonprofits only
    170130-plasmid-map-sequence-id-333605

    Enlarge

  4. pNOC_dCas9_sgRNA Td2

    Plasmid
    #176258
    Purpose
    pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged with Nlux and sgRNA targeting the fluorescent gene tdtomato with spacer 2
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    dead spCas9
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    luciferase
    Expression
    Mutation
    H840A and D10A mutations on spCas9 to inactivate …
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    176258-plasmid-map-sequence-id-347041

    Enlarge

  5. pNOC_dCas9_sgRNA NC

    Plasmid
    #176259
    Purpose
    pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged with Nlux and spacer sequence on sgRNA is replaced by the type IIS restriction site for endonuclease BaeI and
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    dead spCas9
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    luciferase
    Expression
    Mutation
    H840A and D10A mutations on spCas9 to inactivate …
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    176259-plasmid-map-sequence-id-346581

    Enlarge

  10. AA023

    Plasmid
    #215931
    Purpose
    Fragmid fragment: (guide cassette) guide expression; positive control cell surface
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has Service
    Cloning Grade DNA
    Insert
    U6_v1; sgCD81_v1 [Sp]; trRNA_v2 [Sp]; CS2
    Use
    CRISPR; Fragment
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    215931-plasmid-map-sequence-id-431459

    Enlarge

  11. AA145

    Plasmid
    #215937
    Purpose
    Fragmid fragment: (guide cassette) guide expression; Chen F+E trRNA (preferred)
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has Service
    Cloning Grade DNA
    Insert
    U6_v1; BsmBI_v0; BsmBI_v1; trRNA_v4 [Sp]
    Use
    CRISPR; Fragment
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    215937-plasmid-map-sequence-id-431395

    Enlarge

  12. AA232

    Plasmid
    #215940
    Purpose
    Fragmid fragment: (guide cassette) guide expression; positive control cell surface with 2xPP7 & 2xMS2 in trRNA
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has Service
    Cloning Grade DNA
    Insert
    U6_v1; sgCD47_v4 [Sp]; trRNA_v14 [Sp]
    Use
    CRISPR; Fragment
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    215940-plasmid-map-sequence-id-431480

    Enlarge

  13. AA233

    Plasmid
    #215941
    Purpose
    Fragmid fragment: (guide cassette) guide expression; positive control cell surface with 2xPP7 & 2xMS2 in trRNA
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has Service
    Cloning Grade DNA
    Insert
    U6_v1; sgCD274_v1 [Sp]; trRNA_v14 [Sp]
    Use
    CRISPR; Fragment
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    215941-plasmid-map-sequence-id-431532

    Enlarge

  14. AA238

    Plasmid
    #215942
    Purpose
    Fragmid fragment: (guide cassette) guide expression; positive control cell surface
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has Service
    Cloning Grade DNA
    Insert
    U6_v1; sgCD47_v4 [Sp]; trRNA_v4 [Sp]
    Use
    CRISPR; Fragment
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    215942-plasmid-map-sequence-id-431479

    Enlarge

  15. AA242

    Plasmid
    #215943
    Purpose
    Fragmid fragment: (guide cassette) guide expression; positive control cell surface
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has Service
    Cloning Grade DNA
    Insert
    U6_v1; sgCD47_v1 [Sp]; trRNA_v4 [Sp]
    Use
    CRISPR; Fragment
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    215943-plasmid-map-sequence-id-431495

    Enlarge

  16. AA243

    Plasmid
    #215944
    Purpose
    Fragmid fragment: (guide cassette) guide expression; positive control cell surface
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has Service
    Cloning Grade DNA
    Insert
    U6_v1; sgCD47_v5 [Sp]; trRNA_v4 [Sp]
    Use
    CRISPR; Fragment
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    215944-plasmid-map-sequence-id-431494

    Enlarge

  17. AA244

    Plasmid
    #215945
    Purpose
    Fragmid fragment: (guide cassette) guide expression; positive control cell surface
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has Service
    Cloning Grade DNA
    Insert
    U6_v1; sgCD59_v1 [Sp]; trRNA_v4 [Sp]
    Use
    CRISPR; Fragment
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    215945-plasmid-map-sequence-id-431525

    Enlarge

  18. AA246

    Plasmid
    #215946
    Purpose
    Fragmid fragment: (guide cassette) guide expression; positive control cell surface
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has Service
    Cloning Grade DNA
    Insert
    U6_v1; sgCD55_v1 [Sp]; trRNA_v4 [Sp]
    Use
    CRISPR; Fragment
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    215946-plasmid-map-sequence-id-431539

    Enlarge

Showing: 661 - 680 of 868 results