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Plasmid#220460PurposeSplit intein version of Trojan-LexAGAD. Create LexAGAD driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 2 coding intron.DepositorInsertCfaN-LexAGAD-CfaC
UseTagsExpressionInsectMutationPromoterAvailable sinceJune 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiTE(1)-Gal4DBD
Plasmid#220453PurposeSplit intein version of Trojan-Gal4DBD. Create Gal4DBD hemi-driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 1 coding intron.DepositorInsertCfaN-Gal4DBD-CfaC
UseTagsExpressionInsectMutationPromoterAvailable sinceJune 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiTE(1)-LexAGAD
Plasmid#220459PurposeSplit intein version of Trojan-LexAGAD. Create LexAGAD driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 1 coding intron.DepositorInsertCfaN-LexAGAD-CfaC
UseTagsExpressionInsectMutationPromoterAvailable sinceJune 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiTE(0)-p65AD
Plasmid#220455PurposeSplit intein version of Trojan-p65AD. Create p65AD hemi-driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 0 coding intron.DepositorInsertCfaN-p65AD-CfaC
UseTagsExpressionInsectMutationPromoterAvailable sinceJune 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiTE(1)-Gal4
Plasmid#220450PurposeSplit intein version of Trojan-Gal4. Create Gal4 driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 1 coding intron.DepositorInsertCfaN-Gal4-CfaC
UseTagsExpressionInsectMutationPromoterAvailable sinceJune 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiTE(0)-LexAGAD
Plasmid#220458PurposeSplit intein version of Trojan-LexAGAD. Create LexAGAD driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 0 coding intron.DepositorInsertCfaN-LexAGAD-CfaC
UseTagsExpressionInsectMutationPromoterAvailable sinceJune 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiTE(2)-p65AD
Plasmid#220457PurposeSplit intein version of Trojan-p65AD. Create p65AD hemi-driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 2 coding intron.DepositorInsertCfaN-p65AD-CfaC
UseTagsExpressionInsectMutationPromoterAvailable sinceJune 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) ZKSCAN1 MCS-WT Split GFP
Plasmid#69908PurposeExpresses a GFP circular RNA in mammalian cellsDepositorInsertZKSCAN1, GFP (ZKSCAN1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCN-mBFP
Plasmid#63847PurposeEncodes a NADPH dependent blue fluorescent protein expressed under a strong promoter and strong RBS without His-tagged.DepositorInsertmBFP
UseTagsExpressionBacterialMutationPromoter and RBS was swapped. His-tag was removed…PromoterAvailable sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRN3P_T3_ABEmax_IVT
Plasmid#171761PurposePlasmid to be used as DNA template for in-vitro RNA transcription of the ABEmax base editor (A to G) by T3 RNA polymeraseDepositorInsertABEmax
UseVector for in-vitro transcriptionTagsExpressionMutationPromoterT3 promoterAvailable sinceDec. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLD1 translation factors
Plasmid#117760PurposeBioBrick encoding cistrons 1-13 in pET for preparation of PURE 3.0 translation system from E. coliDepositorInsertTranslation factor cistrons 1-13
UseEscherichia coliTagsExpressionMutationPromoterAvailable sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCXLE-dCas9VP192-T2A-EGFP-shP53
Plasmid#69535PurposeEpisomal plasmid encoding dCas9VP192 and p53 shRNADepositorInsertdCas9-dCas9VP192-GFP-shp53
UseCRISPRTagsEGFPExpressionMutationPromoterCAGAvailable sinceNov. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pRK1035
Plasmid#8832DepositorInsertTVMV protease
UseTagsHis and MBP (cleaved)ExpressionBacterialMutationsynthetic gene with codons optimized for E. coliPromoterAvailable sinceFeb. 14, 2006AvailabilityAcademic Institutions and Nonprofits only -
pRN3P_T3_BE4_IVT
Plasmid#171762PurposePlasmid to be used as DNA template for in-vitro RNA transcription of the BE4 base editor (C to T) by T3 RNA polymeraseDepositorInsertBE4
UseVector for in-vitro transcriptionTagsExpressionMutationPromoterT3 promoterAvailable sinceDec. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLD3 translation factors
Plasmid#117762PurposeBioBrick encoding cistrons 22-30 in pET for preparation of PURE 3.0 translation system from E. coliDepositorInsertTranslation factor cistrons 22-30
UseEscherichia coliTagsExpressionMutationPromoterAvailable sinceAug. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLD2 translation factors
Plasmid#117761PurposeBioBrick encoding cistrons 14-21 in pET for preparation of PURE 3.0 translation system from E. coliDepositorInsertTranslation factor cistrons 14-21
UseEscherichia coliTagsExpressionMutationPromoterAvailable sinceAug. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
Cenp-B DBD INCENP GFP
Plasmid#45237PurposeTarget INCENP to centromeres via CENP-B DNA binding domain (DBD)DepositorInsertCenp-B-DBD, INCENP-Δcen, GFP
UseTagsDNA binding domain of CENP-B and GFPExpressionMammalianMutationCEN box deleted from INCENP (aa1-46)PromoterAvailable sinceSept. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-NP
Plasmid#74737Purposemammalian expression of the Nucleoprotein of the A/New Caledonia/20/99 (H1N1) Influenza virusDepositorInsertNucleoprotein
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) ZKSCAN1 MCS-ciRS7 Exon
Plasmid#69906PurposeExpresses the human ciRS7/CDR1as circular RNA in mammalian cellsDepositorInsertciRS7 (CDR1-AS Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBEG R2-L3 #BGV193
Plasmid#48952PurposeFor inserting selection gene between AttR2 and AttL3 sites for use in Gateway recombination cloning system (corresponds to a module 3 plasmid)DepositorTypeEmpty backboneUseGateway cloning vectorTagsExpressionMutationPromoterAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLv-CMV-MyoD
Plasmid#26808DepositorInsertMyoD (Myod1 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-LacI-NLS-VP16
Plasmid#103836PurposeTranscription activator (transactivation domain of viral VP16 protein) that is constitutively recruited lacO arraysDepositorInsertEGFP-LacI-NLS-VP16
UseTagsExpressionMammalianMutationPromoterSV2Available sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pIVT-dCas9-mCherry
Plasmid#174443Purposein vitro transcription template for dCas9-mCherryDepositorInsertCas9 Endonuclease Dead
UseIn vitro transcription templateTagsmCherryExpressionMutationPromoterT7 SP6Available sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
FU-MAP2-Gateway
Plasmid#43915DepositorTypeEmpty backboneUseLentiviral; Gateway destination vectorTagsV5 and non-standard His tag (HHRHHH)ExpressionMammalianMutationPromoterHuman MAP2 promoterAvailable sinceMay 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
MLSE-shREN
Plasmid#105583Purposeretrovirally express control shRNA with GFP markerDepositorInsertcontrol shRNA targeting luciferase
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterMSCV-LTRAvailable sinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
GG-EBNA-OCT4
Plasmid#69537PurposeEpisomal plasmid encoding 5 gRNAS targeting human OCT4 promoterDepositorInsert5 concatenated gRNA transcriptional cassettes targeting OCT4
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET14-GFP30_Encap
Plasmid#86406PurposeExpresses sfGFP tagged with a 30 amino acid CLP and encapsulin as an operonDepositorInsertssfGFP with a 30aa CLP tag
Encapsulin
UseTags6x HistidineExpressionBacterialMutationPromoterT7Available sinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCEP-Puro-TetOn-L1RP-ORF1_R261A_Halo-GFPai
Plasmid#202569PurposeExpresses an endogenous-like L1RP LINE-1 element with a C-terminal HaloTag on the ORF1 protein with the R261A mutation and a GFP retrotransposition reporter (GFP-AI) in the 3' UTRDepositorInsertLINE-1
UseTagsGFP-AI retrotransposition reporter and HaloTag7 o…ExpressionMammalianMutationChanged ORF1p Arginine 261 to AlaninePromoterAvailable sinceApril 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET14-GFP15_Encap
Plasmid#86407PurposeExpresses sfGFP tagged with a 15 amino acid CLP and encapsulin as an operonDepositorInsertssfGFP with a 15aa CLP tag
Encapsulin
UseTags6x HistidineExpressionBacterialMutationPromoterT7Available sinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
KG#121
Plasmid#110879PurposeExpresses the C. elegans unc-31 cDNA pan-neuronallyDepositorInsertsrab-3 promoter
unc-31 cDNA
unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
UseTagsExpressionBacterialMutationMissing first 408bpPromoterAvailable sinceJune 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV-CamK2-tTA
Plasmid#210731PurposeAAV vector expressing tetracycline-controlled transactivator (tTA) under CamK2 promoter (for expressing tTA in glutamatergic neurons)DepositorInserttetracycline-controlled transactivator (tTA)
UseAAVTagsExpressionMutationPromoterCamK2Available sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQCXIP-HA.Tetherin
Plasmid#41069DepositorInsertTetherin (BST2 Human)
UseRetroviralTagsHA and IRES PuroExpressionMammalianMutationPromoterCMV/MSVAvailable sinceNov. 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
p3E-U6a-polg guide
Plasmid#107598PurposeAn entry vector with U6a promoter driving polg guide RNA expressionDepositorInsertpolg guide RNA sequence
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1-GfaABC1D -IgK-TurboID-HA
Plasmid#219810PurposeSecreted HA-tagged TurboID for extracellular proximity labelingDepositorInsertIgK (Igk Mouse)
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
p3E-U6a-U6c
Plasmid#107599PurposeAn entry vector with U6a and U6c promoter driving guide RNAs expressionDepositorInsertsU6a promoter
U6c promoter
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMXs-PT2A-GFP-iRFP670-Tdtomato
Plasmid#111813PurposeTricistronic construct: Co-express three fluorescent proteins by P2A and then T2ADepositorInsertsGFP
iRFP670
Tdtomato
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
ptagBFP-LacI
Plasmid#103839PurposeFlourescent marker for lacO arraysDepositorInserttagBFP-LacI
UseTagsExpressionMammalianMutationLacI: C19T (silent, L7L), T264C (silent, A88A), C…PromoterSV2Available sinceDec. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMXs-PT2A-iRFP670-GFP-Tdtomato
Plasmid#111816PurposeTricistronic construct: Co-express three fluorescent proteins by P2A and then T2ADepositorInsertsiRFP670
GFP
Tdtomato
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
Flag-nls-NgAgo-GK
Plasmid#86435PurposeExpresses a Flag tag on the N-terminalDepositorInsertNgAgo
UseTagsFlag tagExpressionMammalianMutationPromoterCMVAvailable sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
SNV-gfp-IRES-MCS
Plasmid#53122PurposeRetrovirus vector backbone that co-expresses GFP and a gene of interest, driven by the Spleen Necrosis Virus promoter; for use in chicken cellsDepositorTypeEmpty backboneUseRetroviral; For expression in chicken cellsTagsEGFP-IRESExpressionMutationPromoterSNV (spleen necrosis virus)Available sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pT7-th-sgRNA
Plasmid#65565Purposein vitro trancription of sgRNA targeting the zebrafish th locusDepositorInsertth sgRNA (th Zebrafish)
UseIn vitro rna transcriptionTagsExpressionMutationPromoterT7Available sinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRN3P_T3_BE3_IVT
Plasmid#177015PurposePlasmid to be used as DNA template for in-vitro RNA transcription of the BE3 base editor (C to T) by T3 RNA polymeraseDepositorInsertBE3
UseCRISPR; Vector for in-vitro transcriptionTagsExpressionMutationPromoterT3 promoterAvailable sinceDec. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pV2A-T
Plasmid#153022PurposeSingle-plasmid multi-gene expression system suitable for fungi. The obtained polycistronic gene is expressed under the control of a TetON inducible promoterDepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceOct. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTrex-Luc-Phleo
Plasmid#48337PurposeTrasgenically expresses firefly luciferase in T. cruzi with phleomycin selectionDepositorInsertfirely luciferase
UseTrypanasoma cruzi expressionTagsExpressionMutationPromoterT. cruzi rRNAAvailable sinceApril 10, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUAS-FRT-GFP-FRT-uM-dCBEevoCDA1
Plasmid#205152PurposeInducible expression of dCBEevoCDA1 under control of Gal4/UAS and FLP/FRTDepositorInsertFRT-GFP-FRT-uORF-dCBEevoCDA1
UseCRISPRTagsExpressionInsectMutationPromoterUASTAvailable sinceSept. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET14_GFP5_Encap
Plasmid#86408PurposeExpresses sfGFP tagged with a 5 amino acid CLP and encapsulin as an operonDepositorInsertssfGFP with a 5aa CLP tag
Encapsulin
UseTags6x HistidineExpressionBacterialMutationPromoterT7Available sinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
Cenp-B DBD INCENP mCherry
Plasmid#45233PurposeTarget INCENP to centromeres via CENP-B DNA binding domain (DBD)DepositorInsertCenp-B-DBD INCENP-Δcen mCherry
UseTagsDNA binding domain of CENP-B and mCherryExpressionMammalianMutationCEN box deleted from INCENP (aa1-46)PromoterAvailable sinceSept. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
ptagBFP-LacI-NLS-VP16
Plasmid#103837PurposeTranscription activator (transactivation domain of viral VP16 protein) that is constitutively recruited lacO arraysDepositorInserttagBFP-LacI-NLS-VP16
UseTagsExpressionMammalianMutationPromoterSV2Available sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBABE.puro/TO/Flag/tGFP-HuR.wt
Plasmid#110349PurposeDoxycycline inducible mammalian retroviral expression of HuR (wild type) with FLAG and turboGFP tagsDepositorInsertELAV like RNA binding protein 1 (ELAVL1 Human)
UseRetroviralTagsFLAG and tGFPExpressionMammalianMutationPromotergagAvailable sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only