pQCXIP-HA.Tetherin
(Plasmid #41069)

• Depositing Lab: Paul Spearman
• Publication: Hammonds et al PLoS Pathog. 2010 Feb 5;6(2):e1000749.

Backbone

• Vector backbone: pQCXIP
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 7162
• Total vector size (bp): 7700
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Tetherin
• Alt name: BST-2
• Alt name: bone marrow stromal cell antigen 2
• Alt name: CD317
• Species: H. sapiens (human)
• Insert Size (bp): 550
• GenBank ID: NM_004335.2 NP_004326.1
• Entrez Gene: BST2 (a.k.a. CD317, HM1.24, TETHERIN)
• Promoter: CMV/MSV
• Tags / Fusion Proteins:
  • IRES Puro (C terminal on backbone)
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (not destroyed)
• 3′ cloning site: PacI (not destroyed)
• 5′ sequencing primer: LNCX
• 3′ sequencing primer: pCDH-rev (GCATTCCTTTGGCGAGAG)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This is a viral-delivery-compatible HA.Tetherin expression construct. It was generated by cloning of tetherin from plasmid pCMV-HA.Tetherin (Addgene plasmid #41068) into AgeI and PacI restriction sites of pQCXIP (Clontech).