Addgene

We narrowed to 3,174 results for: ado

Showing: 1321 - 1360 of 3174 results

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pLentiCRISPR-v1-sgTK2_5

Plasmid

#217432

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting human TK2

Depositor

Jason Cantor

Article

Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

Insert

sgRNA 5 targeting TK2 (TK2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v1-sgSHMT2

Plasmid

#217435

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting human SHMT2

Depositor

Jason Cantor

Article

Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

Insert

sgRNA targeting SHMT2 (SHMT2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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CLIPf-KIF5A(Δ954-1027)-SNAPf-HisTag-FLAG

Plasmid

#200792

Purpose

Mammalian expression of CLIPf-KIF5A(Δ954-1027)-SNAPf-HisTag-FLAG

Depositor

Alison Twelvetrees

Article

Smith et al bioRxiv 2024.12.20.629623

Insert

KIF5A (Kif5a Mouse)

Use

Tags

CLIPf and SNAPf, HisTag, FLAG

Expression

Mammalian

Mutation

Δ954-1027

Promoter

CMV & T7

Available since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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CLIPf-KIF5A(411-680)-SNAPf-HisTag-FLAG

Plasmid

#200798

Purpose

Mammalian expression of CLIPf-KIF5A(411-680)-SNAPf-HisTag-FLAG

Depositor

Alison Twelvetrees

Article

Smith et al bioRxiv 2024.12.20.629623

Insert

KIF5A (Kif5a Mouse)

Use

Tags

CLIPf and SNAPf, HisTag, FLAG

Expression

Mammalian

Mutation

411-680

Promoter

CMV & T7

Available since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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ZFPL1-GFP

Plasmid

#224590

Purpose

For expression of ZFPL1-GFP in mammalian cells

Depositor

Pietro De Camilli

Article

Ugur et al bioRxiv [Preprint]. 2023 Dec 18:2023.12.

Insert

ZFPL1 (ZFPL1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 20, 2024

Availability

Academic Institutions and Nonprofits only

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FAM177A1-SNAP

Plasmid

#224592

Purpose

For expression of FAM177A1-SNAPTag in mammalian cells

Depositor

Pietro De Camilli

Article

Ugur et al bioRxiv [Preprint]. 2023 Dec 18:2023.12.

Insert

FAM177A1 (FAM177A1 Human)

Use

Tags

SNAP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pEMS2075

Plasmid

#158098

Purpose

High Copy number plasmid containing smCBA insert.

Depositor

Elizabeth Simpson

Article

de Moura Gomes et al Sci Rep. 2024 Nov 15;14(1):28194. doi: 1

Insert

smCBA

Use

Tags

Expression

Mutation

Promoter

smCBA

Available since

Nov. 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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2xCBX7CD_W35A-IFP2.0C

Plasmid

#215755

Purpose

Expresses binding-deficient W35A mutant of 2xCBX7 chromo domain fused to C-terminal part of IFP2.0 as negative control for H3K27me3 detection in BiAD sensors

Depositor

Albert Jeltsch

Article

Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

Insert

2xCBX7CD_W35A-IFP2.0C

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 2, 2024

Availability

Academic Institutions and Nonprofits only

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Q54A

Plasmid

#217912

Purpose

A plasmid encoding the hydrogel-forming protein PXP with the Q54A coil mutation

Depositor

Cole DeForest

Article

Bennett et al Adv Sci (Weinh). 2024 Mar 13:e2301708. d

Insert

PXP Q54A

Use

Tags

6x His (N and C term)

Expression

Bacterial

Mutation

Glutamine at position 55 and 267 mutated to Alani…

Promoter

T5

Available since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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T40A

Plasmid

#217911

Purpose

A plasmid encoding the hydrogel-forming protein PXP with the T40A coil mutation

Depositor

Cole DeForest

Article

Bennett et al Adv Sci (Weinh). 2024 Mar 13:e2301708. d

Insert

PXP T40A

Use

Tags

6x His (N and C term)

Expression

Bacterial

Mutation

Threonine at position 41 and 253 mutated to Alani…

Promoter

T5

Available since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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pILGFPEasy9R

Plasmid

#218291

Purpose

Plasmid-free in-situ promoter cloning and characterisation system in S. cerevisiae, allows the characterisation of a bidirectional promoter using yEGFP and E2-Crimson as reporters.

Depositor

Claudia Vickers

Article

McDonnell et al bioRxiv 2024.03.26.586389

Insert

pURA3>KlURA3>tKlURA3-tPGK1KanMX>tAgTEF-pMAL32>MazF(RPL28i)-E2Crimson>tPDC1-pDDI2>ISceI>tSynth3>ura3(4,191)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 15, 2024

Availability

Academic Institutions and Nonprofits only

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CMV-IgK-pHluorin-TM-mRuby

Plasmid

#185546

Purpose

Expresses surface-localized pHluorin fused to intracellular expression of mRuby under CMV promoter

Depositor

Jaime de Juan-Sanz

Article

Cuhadar et al Cell Rep. 2024 May 28;43(5):114186. doi:

Insert

IgK-pHluorin-TM-mRuby

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

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2x3APWWP_K299E_D333A-IFP2.0C

Plasmid

#215753

Purpose

Expresses binding-deficient K299E/D333A mutant of 2xDNMT3A PWWP domain fused to C-terminal part of IFP2.0 as negative control for H3K36me2/3 detection in BiAD sensors

Depositor

Albert Jeltsch

Article

Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

Insert

2x3APWWP_K299E_D333A-IFP2.0C

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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pNHT7::UMSBP::sNluc2_ORF1

Plasmid

#186765

Purpose

An mRNA production vector encoding planarian codon-optimized sNluc2 with UMSBP 5' and 3' UTRs beginning at the endogenous gene's start codon.

Depositor

Bo Wang

Article

Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

Insert

Nanoluciferase

Use

Tags

Expression

Mutation

Promoter

T7

Available since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pNHT7::ENO::sNluc2

Plasmid

#186770

Purpose

An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc2) with enolase 5' and 3' UTRs

Depositor

Bo Wang

Article

Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

Insert

Nanoluciferase

Use

Tags

Expression

Mutation

Promoter

T7

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pNHT7::RPL15::sNluc0

Plasmid

#186771

Purpose

An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc0) with RPL15 5' and 3' UTRs

Depositor

Bo Wang

Article

Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

Insert

Nanoluciferase

Use

Tags

Expression

Mutation

Promoter

T7

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pNHT7::RPL15::hNluc

Plasmid

#186772

Purpose

An mRNA production vector encoding human codon-optimized nanoluciferase (hNluc) with RPL15 5' and 3' UTRs

Depositor

Bo Wang

Article

Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

Insert

Nanoluciferase

Use

Tags

Expression

Mutation

Promoter

T7

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pNHT7::YB1::hNluc

Plasmid

#186774

Purpose

An mRNA production vector encoding human codon-optimized nanoluciferase (hNluc) with y-box binding protein 5' and 3' UTRs

Depositor

Bo Wang

Article

Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

Insert

Nanoluciferase

Use

Tags

Expression

Mutation

Promoter

T7

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pGAPDH::sNluc1

Plasmid

#187221

Purpose

A plasmid containing a planarian codon-optimized Nluc (sNluc1), driven by the planarian GAPDH promoter.

Depositor

Bo Wang

Article

Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

Insert

Nanoluciferase

Use

Overexpression

Tags

Expression

Mutation

Promoter

GAPDH

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pNHT7::YB1::sNluc1

Plasmid

#186754

Purpose

An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc1, CAI = 0.925) with y-box binding protein 5' and 3' UTRs.

Depositor

Bo Wang

Article

Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

Insert

Nanoluciferase

Use

Tags

Expression

Mutation

Promoter

T7

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pNHT7::ENO::sNluc1

Plasmid

#186755

Purpose

An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc1) with enolase 5' and 3' UTRs.

Depositor

Bo Wang

Article

Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

Insert

Nanoluciferase

Use

Tags

Expression

Mutation

Promoter

T7

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pNHT7::RPL10::sNluc1

Plasmid

#186756

Purpose

An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc1) with RPL10 5' and 3' UTRs.

Depositor

Bo Wang

Article

Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

Insert

Nanoluciferase

Use

Tags

Expression

Mutation

Promoter

T7

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pNHT7::RPL15::sNluc1

Plasmid

#186757

Purpose

An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc1) with RPL15 5' and 3' UTRs.

Depositor

Bo Wang

Article

Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

Insert

Nanoluciferase

Use

Tags

Expression

Mutation

Promoter

T7

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pNHT7::YB1::sNluc0

Plasmid

#186758

Purpose

An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc0, CAI = 1.0) with y-box binding protein 5' and 3' UTRs.

Depositor

Bo Wang

Article

Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

Insert

Nanoluciferase

Use

Tags

Expression

Mutation

Promoter

T7

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pNHT7::YB1::sNluc2

Plasmid

#186759

Purpose

An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc2, CAI =0.713) with y-box binding protein 5' and 3' UTRs.

Depositor

Bo Wang

Article

Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

Insert

Nanoluciferase

Use

Tags

Expression

Mutation

Promoter

T7

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pNHT7::YB1::sNluc3

Plasmid

#186760

Purpose

An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc3, CAI = 0.663) with y-box binding protein 5' and 3' UTRs.

Depositor

Bo Wang

Article

Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

Insert

Nanoluciferase

Use

Tags

Expression

Mutation

Promoter

T7

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pNHT7::YB1::sNluc4

Plasmid

#186761

Purpose

An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc4, CAI = 0.743) with y-box binding protein 5' and 3' UTRs.

Depositor

Bo Wang

Article

Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

Insert

Nanoluciferase

Use

Tags

Expression

Mutation

Promoter

T7

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pNHT7::YB1::sNluc5

Plasmid

#186762

Purpose

An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc5, CAI = 0.633) with y-box binding protein 5' and 3' UTRs.

Depositor

Bo Wang

Article

Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

Insert

Nanoluciferase

Use

Tags

Expression

Mutation

Promoter

T7

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pNHT7::RPL15::sNluc2_STOP

Plasmid

#186767

Purpose

An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc2) containing a premature stop codon.

Depositor

Bo Wang

Article

Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

Insert

Nanoluciferase

Use

Tags

Expression

Mutation

Premature stop codon at residue 16

Promoter

T7

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pNHT7::RPL15::sGluc

Plasmid

#186769

Purpose

An mRNA production vector encoding planarian codon-optimized gaussia luciferase (sGluc) with RPL15 5' and 3' UTRs.

Depositor

Bo Wang

Article

Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

Insert

Gaussia luciferase

Use

Tags

Expression

Mutation

Promoter

T7

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDF11-SFPQ(214-535)

Plasmid

#135439

Purpose

Expresses SFPQ 214-535 with TEV-cleavable hexahistidine tag

Depositor

Charles Bond

Article

Lee et al Nucleic Acids Res. 2015 Apr 20;43(7):382

Insert

splicing factor proline and glutamine rich (SFPQ Human)

Use

Tags

Hexahistidine

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 15, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pSUPER.retro.puro-shLARP7

Plasmid

#113537

Purpose

Retroviral vector designed to knock down LARP7.

Depositor

Blerta Xhemalce

Article

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

shLARP7 (LARP7 Human)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-5X-1-MePCE_aa470-689

Plasmid

#113544

Purpose

Produces an N-terminal GST-tagged MePCE protein consisting of amino acids 470 - 689.

Depositor

Blerta Xhemalce

Article

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

MePCE (MEPCE Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-5X-1_MePCE_aa432-689

Plasmid

#113543

Purpose

Produces an N-terminal GST-tagged MePCE protein consisting of amino acids 432 - 689.

Depositor

Blerta Xhemalce

Article

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

MePCE (MEPCE Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-5X-1_MePCE_aa400-689

Plasmid

#113542

Purpose

Produces an N-terminal GST-tagged MePCE protein consisting of amino acids 400 - 689.

Depositor

Blerta Xhemalce

Article

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

MePCE (MEPCE Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-5X-1_MePCE_aa1-400

Plasmid

#113541

Purpose

Produces an N-terminal GST-tagged MePCE protein consisting of amino acids 1 - 400.

Depositor

Blerta Xhemalce

Article

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

MePCE (MEPCE Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
PX458_MIER3_2

Plasmid

#86324

Purpose

Encodes gRNA for 3' target of human MIER3

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against MIER3 (MIER3 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
PX458_KDM3A_1

Plasmid

#86321

Purpose

Encodes gRNA for 3' target of human KDM3A

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against KDM3A (KDM3A Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only
PX458_HMG20A_2

Plasmid

#86319

Purpose

Encodes gRNA for 3' target of human HMG20A

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against HMG20A (HMG20A Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
PX458_KLF11_1

Plasmid

#86314

Purpose

Encodes gRNA for 3' target of human KLF11

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against KLF11 (KLF11 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 1321 - 1360 of 3174 results

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