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pCAGGS EFF-1::V5

Plasmid

#133584

Purpose

Expresses EFF-1 A full length protein in mammalian cells

Depositor

Benjamin Podbilewicz

Article

Avinoam et al Science. 2011 Apr 29;332(6029):589-92. d

Insert

EFF-1::V5

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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punc-119c

Plasmid

#21595

Depositor

Al Fisher

Article

Ferguson et al Plasmid. 2009 Jun 8. ():.

Insert

unc-119 (unc-119 Nematode)

Use

Adds unc-119 marker to amp resistant plasmids

Tags

Expression

Worm

Mutation

N/A

Promoter

Available since

Aug. 26, 2009

Availability

Academic Institutions and Nonprofits only

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pCM1.153

Plasmid

#21387

Depositor

Geraldine Seydoux

Article

Merritt et al Curr Biol. 2008 Sep 23. ():.

Insert

gld-1 M1M2 3'utr in pDONRP2RP3

Use

Gateway

Tags

Expression

Mutation

Promoter

Available since

June 19, 2009

Availability

Academic Institutions and Nonprofits only

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pCM3.11

Plasmid

#19605

Depositor

Geraldine Seydoux

Insert

pgl-1 ORF+3' in pDONR201 (pgl-1 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Nov. 10, 2008

Availability

Academic Institutions and Nonprofits only

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pJL1604

Plasmid

#78194

Purpose

Ce cha-1 expression in motor neurons in worms

Depositor

Junho Lee

Article

Lee et al Nat Neurosci. 2011 Nov 13;15(1):107-12.

Insert

cha-1 (cha-1 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

unc-3

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only
pGC49

Plasmid

#19680

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

Gateway(R1-R2)-L4440

Use

RNAi; Gateway destination vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 25, 2009

Availability

Academic Institutions and Nonprofits only

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pGC133

Plasmid

#19612

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

Phsp-16.41::FLP::let-858(3’)-(Cbr)unc-119(+)

Use

Flp/frt

Tags

Expression

Worm

Mutation

Promoter

Available since

March 5, 2009

Availability

Academic Institutions and Nonprofits only

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pJW1820

Plasmid

#154321

Purpose

Multi-cassette for SapTrap

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

GFP^SEC (LoxP)^AID*::3xFLAG

Use

CRISPR and Cre/Lox

Tags

Expression

Worm

Mutation

Promoter

Available since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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BSP605

Plasmid

#122250

Purpose

expression vector for mCerulean under EFT3 promoter

Depositor

Alexander Mendenhall

Article

Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

Insert

mCerulean

Use

Tags

Expression

Worm

Mutation

Promoter

EFT3

Available since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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pRG02

Plasmid

#44196

Depositor

Denis Dupuy

Insert

Neo

Use

Tags

Expression

Worm

Mutation

Promoter

rps-27

Available since

April 10, 2013

Availability

Academic Institutions and Nonprofits only

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pBN266

Plasmid

#86732

Purpose

Tissue-specific FLP expression plasmid for C. elegans

Depositor

Peter Askjaer

Article

Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

Insert

Pdpy-7::FLP D5

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Feb. 7, 2017

Availability

Academic Institutions and Nonprofits only

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TC393

Plasmid

#122625

Purpose

Pser-2::rtetR-QFAD::P2A::mKate::T2A::tetR-pie1

Depositor

Tian Chi

Article

Mao et al iScience. 2019 Jan 25;11:224-237. doi: 1

Insert

rtetR-QFAD::P2A::mKate::T2A::tetR-pie1

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

May 28, 2019

Availability

Academic Institutions and Nonprofits only

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pDP-let-7_LPB

Plasmid

#26560

Depositor

Chang-Zheng Chen

Article

Trujillo et al EMBO J. 2010 Aug 31. ():.

Insert

cel-pre-let-7_LPB

Use

Transcription vector

Tags

Expression

Mutation

loop mutant B

Promoter

Available since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only

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pJN601

Plasmid

#59790

Purpose

zf1::yfp fusion with floxed unc-119(+) within an intron of yfp. For creating zf1::yfp knock-ins using unc-119 as a marker

Depositor

Jeremy Nance

Article

Armenti et al Development. 2014 Dec;141(23):4640-7. do

Inserts

ZF1 domain from pie-1
N-terminal half of yfp with introns
intron containing floxed unc-119 gene in reverse orientation
C-terminal half of yfp with introns

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Oct. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pCM1.111

Plasmid

#19598

Depositor

Geraldine Seydoux

Insert

mex-5 prom in PDONRP4P1R

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Nov. 10, 2008

Availability

Academic Institutions and Nonprofits only

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pCM3.10

Plasmid

#19604

Depositor

Geraldine Seydoux

Insert

pgl-3 ORF+3' in pDONR201 (pgl-3 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Nov. 10, 2008

Availability

Academic Institutions and Nonprofits only

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pCFJ1360 - Intron GG3 - smu-1

Plasmid

#159806

Purpose

Golden Gate compatible intron with PATCs for reduced germline silencing

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

Intron GG3 - smu-1

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pMDJ15 - pEXP[Peft-3 | mCardinal (NLS) | tbb-2 UTR]

Plasmid

#159801

Purpose

Expression construct with ubiquitous expression to test fluorescence level on microscope

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP[Peft-3 | mCardinal (NLS) | tbb-2 UTR]

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCFJ1358 - Intron GG1 - smu-1

Plasmid

#159804

Purpose

Golden Gate compatible intron with PATCs for reduced germline silencing

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

Intron GG1 - smu-1

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pJW1851

Plasmid

#154329

Purpose

sgRNA Target Vector for CRISPR

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

cxTi10882 site targeting sgRNA (F+E) with K09B11.2 U6 promoter and 3'UTR

Use

CRISPR

Tags

Expression

Worm

Mutation

F+E mutations in sgRNA

Promoter

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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