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Plasmid#179702Purposevisualization of centromere heterochromatinDepositorInsertheterochromatin protein alpha (CBX5 Human)
UseTagsdsRedExpressionMammalianMutationPromoterAvailable sinceMarch 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBabe-PI(WT)-Rac1(Q61L)-mCherry
Plasmid#87553PurposePI(WT): Photo-inhibitable/blue light sensitive; const. active Rac1DepositorInsertsUseTagsmCherryExpressionBacterial and MammalianMutationQ61LPromoterCMVAvailable sinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pQStrep2-GADD45G
Plasmid#31584DepositorInsertgrowth arrest and DNA-damage-inducible, gamma (GADD45G Human)
UseTagsHis and StrepExpressionBacterialMutationPromoterAvailable sinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBABEpuro-ARAF_S214C
Plasmid#51077Purposemammalian expression of ARAF S214CDepositorInsertARAF (ARAF Human)
UseRetroviralTagsExpressionMammalianMutationS214CPromoterAvailable sinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
CABE-T2.6
Plasmid#193263PurposeExpression of CABE-T2.6 base editor in mammalian cells, CMV promoterDepositorInsertCABE-T2.6
UseTagsExpressionMammalianMutationD10A (Cas9), TadA mutations withinPromoterCMVAvailable sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPSP6+t-gfp
Plasmid#48130PurposeShuttle vector E. coli/B.meg.; gfp-expression under control of RNAP-SP6-inducible promoterDepositorInsertgfp
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
CBE-T1.6
Plasmid#193275PurposeExpression of CBE-T1.6 base editor in mammalian cells, CMV promoterDepositorInsertCBE-T1.6
UseTagsExpressionMammalianMutationD10A (Cas9), TadA mutations withinPromoterCMVAvailable sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-MtPylRS (human-opti)
Plasmid#164080PurposeTo express a Methanosarcina thermophila derived PylRS in mammalian cells for genetic code expansionDepositorInsertMtPylRS-478(human-opti)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-MfBulKRS (human-opti)
Plasmid#164196PurposeTo express a Methanosarcina flavescens derived BulKRS in mammalian cells for genetic code expansionDepositorInsertMf BulKRS(human-opti)
UseSynthetic BiologyTagsExpressionMammalianMutationY269A;Y347FPromoterCMVAvailable sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
LN-entry
Plasmid#128012PurposeVector containing the lambdaN-entry expression cassette and a separate expression cassette driving mCherry via the traffic jam enhancerDepositorInsertLN-3xFlag
UseLamdan entry vectorTagsLN-3xFlagExpressionMutationPromoterAvailable sinceAug. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBS953 TARDBP-Exon2-ko-sgRNA2-SpCas9
Plasmid#107858PurposeTo knock-out TDP43DepositorInsertgRNA against TARDBP (Exon 2) (TARDBP Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2 mir-1-1 reporter hsa-mir-92a-1
Plasmid#46672DepositorInserthsa-mir-92a-1 (MIR92A1 Human)
UseRNAiTagsV5 and hsa-mir-1-1ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-ILKAP
Plasmid#34817DepositorInsertintegrin-linked kinase-associated serine/threonine phosphatase 2C (ILKAP Human)
UseTagsHis and TEVExpressionBacterialMutationPromoterAvailable sinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEasyG2_mic
Plasmid#184916PurposeTemplate to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_mic recombines in vivo with a PCR product from pEasyG2_zeo/nat/hph.DepositorInsertgRNA scaffold
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS/U6/RNAi SadBs shRNA mouse
Plasmid#67152PurposepBS/U6 containing shRNA to mouse SADBsDepositorInsertBRSK1 (Brsk1 Mouse)
UseTagsExpressionMammalianMutationPromoterU6Available sinceNov. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLX-B3omega2 (GB1695)
Plasmid#160644PurposepLX series: pBBR1-based T-DNA binary vector for plant transformation (omega2)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLX-B2alpha2 (GB1696)
Plasmid#160620PurposepLX series: pBBR1-based T-DNA binary vector for plant transformation (alpha2)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAD-CMV-STAT5A-DN-CMV-GFP
Plasmid#83252PurposeAdenoviral expression of STAT5A dominant negative with co-expression of GFPDepositorInsertsUseAdenoviralTagsExpressionMutationC-terminal deletion of 135 nucleotides (dominant …PromoterCMVAvailable sinceSept. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMC234r-ccdB/TPK2
Plasmid#176179Purposeyeast MoClo level-0 part plasmid type 234r containing toxic gene dropout cassette (ccdB and TPK2)DepositorInsertdropout cassette containing toxic genes ccdB and TPK2
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTargeting_Mettl3C_FKBP-V
Plasmid#165421PurposeTemplate for knockin FBKP-V into Mettl3 C-terminusDepositorInsertMettl3 C-terminus + linker + FKBP-V (Mettl3 Mouse)
UseMouse TargetingTagsFKBP-VExpressionMutationPromoterAvailable sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only