-
Plasmid#62747PurposeLocalization: Kinesin Light Chain , N1 Cloning Vector, Excitation:587, Emission:610DepositorInsertKLC2
UseTagsmCherryExpressionMammalianMutationnonePromoterCMVAvailable sinceApril 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CHRM3-cpHalo∆-mEGFP
Plasmid#194678PurposeCMV driven expression of the m3 GPCR recorder in mammalian cell lines fused to mEGFPDepositorInsertCHRM3_cpHalo∆
UseTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFRLuc-CoV2-FLucStop
Plasmid#177617PurposeDual luciferase reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting. In-frame stop codon inserted after FLuc (negative control).DepositorInsertSARS-CoV-2 FSE
UseLuciferaseTagsExpressionMammalianMutationIn-frame stop codon added between FLuc and FSEPromoterAvailable sinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-FRT-Rev-3xGFP
Plasmid#191204PurposeFlpO dependent GFP expressionDepositorInsert3X GFP
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJRD215
Plasmid#167555PurposeBackbone plasmid to pRMA154DepositorTypeEmpty backboneUseBroad host range mobilisableTagsExpressionMutationPromoterAvailable sinceMay 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTECH-chPylRS(IPYE)
Plasmid#99222PurposeExpression plasmid encoding chPylRS(IPYE) and pylTDepositorInsertPlpp chPyl(IPYE)RS; PproK pylT
UseTagsExpressionBacterialMutationContains activating mutations V31I, T56P, H62Y, A…PromoterPlpp and PproKAvailable sinceOct. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
mCarmine in pcDNA3
Plasmid#109486Purposemammalian expression vector for mCarmine, a far red emitting fluorescent protein (excitation: 603; emission: 675)DepositorInsertmCarmine
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-TRE3G-NFIA+SOX9
Plasmid#129455PurposeInducible overexpression of NFIA and Sox9 in human cells.DepositorInsertSox 9 (SOX9 Human)
UseTagsExpressionMammalianMutationPromoterTRE3GAvailable sinceMarch 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTK305
Plasmid#66812PurposeIVT vector for Fluc mRNADepositorInsertFirefly luciferase
UseLuciferase; Mrna ivtTagsExpressionMutationPromoterT7Available sinceSept. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEG BacMam C term 3C eGFP His8
Plasmid#160687PurposeVector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target proteinDepositorTypeEmpty backboneUseTags3C eGFP His8ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pL0M-S-mNeonGreen-EC18153
Plasmid#137075PurposeGolden Gate Level 0 S module for N-terminal fluorescent protein taggingDepositorInsertmNeonGreen DNA synthesis product with 5' and 3' extensions for Golden Gate cloning
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-EF1a-BsdCas9-W
Plasmid#67978PurposeLentiviral vector expressing Cas9 fused with the Blasticidin resistant gene at the N-terminusDepositorInsertEF1a-Cas9 cassette, WPRE
UseCRISPR and LentiviralTagsCas9 fused with Flag-tag and a NLS at the N termi…ExpressionMutationPromoterEF1aAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLV CMV-TERRA
Plasmid#111375PurposeTERRA expression contains approximately 130 telomere hexanucleotide repeats of TTAGGG and TTGGGG variant sequencesDepositorInsertTERRA polyA
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceSept. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
EGFR (del3) L747-E749del, A750P
Plasmid#11015PurposeRetroviral construct for expressing human EGFR with L747-E749 deletion and A750P mutation in human cellsDepositorInsertEGFR (del3) (EGFR Human)
UseRetroviralTagsExpressionMammalianMutationL747-E749 deleted, A750PPromoterAvailable sinceDec. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-FLT3-V5/HIS
Plasmid#201985Purposeexpression of human FLT3 receptor tyrosine kinase in mammalian cellsDepositorInsertFLT3 receptor tyrosine kinase (FLT3 Human)
UseTagsV5, 6xHisExpressionMammalianMutationPromoterCMVAvailable sinceJune 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
Rab5a-pmCherryC1
Plasmid#27679DepositorInsertRab5a (Rab5a Mouse)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceMay 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLNCX2-CFP-VGPDFGR-YFP
Plasmid#59587PurposeEncodes a granzyme B FRET reporterDepositorInsertECFP-VGPDFGR-Venus
UseRetroviralTagsECFP and VenusExpressionMammalianMutationPromoterCMVAvailable sinceNov. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV Blast empty (w263-1)
Plasmid#17486Purpose3rd gen lentiviral empty control vector, CMV promoter, BlastDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
hE-cadherin-pcDNA3
Plasmid#45769DepositorInserthE-cadherin (CDH1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Akt-Scramble
Plasmid#175014PurposeNegative-control construct for Akt-STOPS.DepositorInsertmCherry-Akt-Scramble
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only