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Plasmid#125076PurposeExpression of ompR under PLtetO-1, output promoter PompF146DepositorInsertsUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 28, 2019AvailabilityAcademic Institutions and Nonprofits only
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AAV.CBA.eGFP.2A.wtTau
Plasmid#140424PurposeAAV.CBA.eGFP.2A.wtTau propagation reporter WT-2N4R-hTauDepositorInserteGFP-2A-Tau (MAPT Human, Synthetic)
UseAAVTagseGFPExpressionMutationPromoterCBAAvailable sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
C1(1-29)-TurboID-V5_pLX304
Plasmid#107175Purposeexpresses V5-tagged TurboID on the mammalian ER membrane, lentiviral vectorDepositorInsertTurboID (BirA mutant)
UseLentiviralTagsExpressionMutationQ65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…PromoterAvailable sinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
tdTomato-pBAD
Plasmid#54856PurposeLocalization: Protein Expression Vector , Excitation: 554, Emission: 581DepositorTypeEmpty backboneUseTags6xHis and tdTomatoExpressionBacterialMutationPromoterAvailable sinceJuly 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
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YFVdelNS1-Cre
Plasmid#179952PurposepCCI vector encoding YFV-17D lacking nonstructural protein 1 (NS1) but with CreDepositorInsertYFV-17D lacking nonstructural protein 1 (NS1), but with Cre
UseTransneuronal tracingTagsExpressionMutationPromoterSP6Available sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAC-ZEAXipi
Plasmid#53287PurposeContains crtE, idi, crtI, crtY, crtB, and crtZ genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces zeaxanthin in E. coliDepositorInsertcrtE, idi, crtY, crtI, crtB, crtZ
UseLow copy number bacterial cloning vectorTagsExpressionMutationPromoterendogenous promotersAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGFP-Cytochrome C
Plasmid#41181DepositorInsertCytochrome C (CYCS Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA1-Tat
Plasmid#138478PurposeExpresses HIV Tat for efficient sgRNA packaging.DepositorInsertTat
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV/D374Y-hPCSK9
Plasmid#58379PurposeExpresses GoF mutant human PCSK9 to be used for rAAV8-mediated gene transfer, hypercholesterolemia and atherosclerosisDepositorInsertproprotein convertase subtilisin/kexin type 9 (PCSK9 Human)
UseAAVTagsExpressionMammalianMutationD374YPromoterHCRApoE/hAATAvailable sinceAug. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCryptDel4.8
Plasmid#141293PurposePlasmid for curing pMUT2 in one step based on pFREE. Contains RelB antitoxin, as well as gRNA targetting pMUT2 plasmid as well as pCryptDel4.8 itself.DepositorInsertsRelB
gRNA targetting pMUT2
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterNative promoter from pMUT2 relB/relE operon and P…Available sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTetON-EGFP
Plasmid#89453PurposeTet/Dox-inducible expression of EGFP in mammalian cells (control)DepositorInsertEGFP
UseTagsExpressionMammalianMutationPromoterTRE3GAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
gRNA_Cloning Vector BbsI
Plasmid#128433PurposeEmbty backbone vector for expression of gRNA for spCas9 genome editing or epigenome editingDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterU6 promoterAvailable sinceJuly 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAGIG
Plasmid#11159PurposeMammalian expression vector for cloning and expressing your gene on a bicistronic mRNA also encoding IRES-GFPDepositorHas ServiceCloning Grade DNAInsertIRES-EGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 13, 2006AvailabilityAcademic Institutions and Nonprofits only -
pDONRp5-p2 GFP-NShroom3-iLID
Plasmid#170976PurposeN-terminal component of OptoShroom3 optogenetic tool. Binds to apical junctions. pDONRp5-p2 plasmid.DepositorInsertNShroom3 (Shroom3 Mouse)
UseGateway cloningTagseGFP and iLIDExpressionMutationFrom isoform 2, deleted amino acids 1389 - 1805,…PromoterAvailable sinceJuly 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONRp5-p2 SspB-mCherry-CShroom3
Plasmid#170977PurposeC-terminal component of OptoShroom3 optogenetic tool. Translocates to apical junctions to induce constriction upon blue light illumination if coexpressed with GFP-NShroom3-iLID. pDONRp5-p2 plasmid.DepositorInsertCShroom3 (Shroom3 Mouse)
UseGateway cloningTagsSspB and mCherryExpressionMutationIsoform X2, deleted aminoacids 1 - 1388PromoterAvailable sinceJuly 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCXLE-mCherry-t2a-SOX2-17-p2a-KLF4
Plasmid#193294Purposeepisomal mCherry-hS*K (expressing mCherry + human super-SOX + KLF4) for integration-free naïve conversionDepositorUseTagsExpressionMammalianMutationSOX2-17 is engineered highly cooperative chimeric…PromoterCAGAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pATetO 6XHis-Taq DNA Ligase
Plasmid#178798PurposeInducible expression of Taq DNA Ligase in E. coliDepositorInsertTaq DNA Ligase
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-WT-STAT1
Plasmid#71454PurposeLentiviral expression of WT-STAT1DepositorInsertSTAT1 (STAT1 Human)
UseLentiviralTagsFLAGExpressionMutationPromoterCMVAvailable sinceDec. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR
Plasmid#118154PurposeCatalytically inactive Cas9 from S. pyogenes with P2A-BlastR under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.DepositorInsertKRAB-dCas9-P2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianMutationSee depositor comments belowPromoterEF1a core and U6Available sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only