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pJW1669

Plasmid

#112043

Purpose

CEN/ARS plasmid for GAL-inducible NSG2-V5-TEV-EGFP expression marked with URA

Depositor

Jonathan Weissman

Article

Costa et al Science. 2018 Jan 18. pii: science.aar36

Insert

NSG2

Use

Tags

V5-TEV-EGFP

Expression

Yeast

Mutation

Promoter

GAL1

Available since

Availability

Academic Institutions and Nonprofits only

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pJW1672

Plasmid

#112046

Purpose

CEN/ARS plasmid for GAL-inducible PST1-V5-TEV-EGFP expression marked with URA

Depositor

Jonathan Weissman

Article

Costa et al Science. 2018 Jan 18. pii: science.aar36

Insert

PST1

Use

Tags

V5-TEV-EGFP

Expression

Yeast

Mutation

Promoter

GAL1

Available since

Availability

Academic Institutions and Nonprofits only

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pJW1674

Plasmid

#112048

Purpose

CEN/ARS plasmid for GAL-inducible SWP1-V5-TEV-EGFP expression marked with URA

Depositor

Jonathan Weissman

Article

Costa et al Science. 2018 Jan 18. pii: science.aar36

Insert

SWP1

Use

Tags

V5-TEV-EGFP

Expression

Yeast

Mutation

Promoter

GAL1

Available since

Availability

Academic Institutions and Nonprofits only

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pVW296

Plasmid

#139496

Purpose

Expression vector for the PP7-mCherry protein in S. cerevisiae

Depositor

Serge Pelet

Article

Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

Insert

pADH1 PP7∆FG-mCherry tCYC1

Use

Tags

Expression

Yeast

Mutation

∆FG

Promoter

pADH1

Available since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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pHLM

Plasmid

#64168

Purpose

harbours marker genes HIS3, LEU2 and MET17

Depositor

Markus Ralser

Article

Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

Type

Empty backbone

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pFLAG UBR1 SBX

Plasmid

#24506

Depositor

Alexander Varshavsky

Article

Xia et al J Biol Chem. 2008 Aug 29. 283(35):24011-

Insert

UBR1 (UBR1 Budding Yeast)

Use

Tags

FLAG

Expression

Yeast

Mutation

Promoter

Available since

May 1, 2015

Availability

Academic Institutions and Nonprofits only
pKmK.I1L

Plasmid

#125030

Purpose

Storage plasmid for K.marxianus left homology arm; type 1 part

Depositor

John Morrissey

Article

Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

Insert

Left homology arm for targeting integration to LAC4

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pKmK.I3L

Plasmid

#125032

Purpose

Storage plasmid for K.marxianus left homology arm; type 1 part

Depositor

John Morrissey

Article

Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

Insert

Left homology arm for targeting integration to SWF1/ARO1

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pyEvolvR (enCas9-PolI5M)

Plasmid

#158073

Purpose

Expresses enCas9 fused to PolI5M in S. cerevisiae; contains a BsmbI-flanked GFP cassette for sgRNA cloning.

Depositor

John Dueber

Article

Tou et al ACS Synth Biol. 2020 Jun 16. doi: 10.102

Insert

enCas9-PolI5M

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pSAP

Plasmid

#206429

Purpose

A vector backbone domesticated for SapI (pCC1BAC-based), contains elements for selection, maintenance, and propagation in S. cerevisiae (TRP1) and E. coli (cat).

Depositor

Bogumil Karas

Article

Walker et al Plant Physiol. 2023 Dec 19:kiad670. doi:

Type

Empty backbone

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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p413_Csy4NLS

Plasmid

#103139

Purpose

Expresses Csy4-NLS under TEF promoter

Depositor

Jens Nielsen

Article

Ferreira et al ACS Synth Biol. 2017 Nov 21. doi: 10.102

Insert

Csy4

Use

Tags

NLS

Expression

Yeast

Mutation

Promoter

TEF

Available since

Jan. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pINTO_7/8

Plasmid

#206431

Purpose

A vector backbone (pCC1BAC-based) containing a single SapI recognition site. When digested, it exposes overhangs that allow integration of the vector into the P. tricornutum chloroplast genome.

Depositor

Bogumil Karas

Article

Walker et al Plant Physiol. 2023 Dec 19:kiad670. doi:

Type

Empty backbone

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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p413TEF-Sir2

Plasmid

#51742

Purpose

overexpression of Sir2 in S. cerevisiae, HIS3 marker

Depositor

Markus Ralser

Article

Ralser et al PLoS One. 2009;4(4):e5376. doi: 10.1371/

Insert

Sir2 (SIR2 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

TEF1

Available since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

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pNB768

Plasmid

#60139

Purpose

Green click beetle luciferase from Promega driven by MET17 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

CBG99

Use

Tags

Expression

Yeast

Mutation

Promoter

MET17pr

Available since

Nov. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pHLU

Plasmid

#64167

Purpose

harbours marker genes HIS3, LEU2 and URA3

Depositor

Markus Ralser

Article

Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

Type

Empty backbone

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pPS2351

Plasmid

#1352

Depositor

Pamela Silver

Article

Hitchcock et al Mol Biol Cell 2001 Oct;12(10):3226-41.

Insert

MGA2 (MGA2 Budding Yeast)

Use

Tags

GFP

Expression

Yeast

Mutation

Promoter

Available since

Aug. 3, 2005

Availability

Academic Institutions and Nonprofits only
pPS2348

Plasmid

#1351

Depositor

Pamela Silver

Article

Hitchcock et al Mol Biol Cell 2001 Oct;12(10):3226-41.

Insert

SPT23 (SPT23 Budding Yeast)

Use

Tags

GFP

Expression

Yeast

Mutation

Promoter

Available since

Aug. 3, 2005

Availability

Academic Institutions and Nonprofits only
pSFS2A-CaKan

Plasmid

#189565

Purpose

Codon optomized Kan resistance gene in the pSFS2A flipper backbone for genetic manipulation of Candida albicans using G418 selection

Depositor

Richard Bennett

Article

Park et al mSphere. 2022 Aug 15:e0034722. doi: 10.1

Insert

CaKan

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pPM47 (UPR-RFP CEN/ARS URA3)

Plasmid

#20132

Depositor

Feroz Papa

Article

Merksamer et al Cell. 2008 Nov 28. 135(5):933-47.

Insert

UPR-RFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 11, 2009

Availability

Academic Institutions and Nonprofits only

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CelTag Plasmid

Plasmid

#66562

Purpose

Encodes CelTag for the tagging and high affinity purification of endogenous yeast proteins.

Depositor

David Engelke

Article

Carrick et al G3 (Bethesda). 2015 Dec 29. pii: g3.115.

Insert

CelTag

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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