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pCDNA3.0_mitoLAMA-G97 IRES ShadowG-mScarlet
Plasmid#130709PurposePlasmid encoding outer mitochondrial localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and ShadowG-mScarletDepositorInsertNTOM20-SNAP-Tag-LAMA-G97 IRES ShadowG-mScarlet
UseTagsNTOM20 and SNAP-TagExpressionMammalianMutationPromoterAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
GFP-nArgBP2ΔNSE
Plasmid#74515PurposeExpress GFP-nArgBP2ΔNSE fusion protein in mammalian cells. nArgBP2ΔNSE is the mutant without the neuronal specific exon of nArgBP2.DepositorInsertnArgBP2delNSE (Sorbs2 Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceJuly 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFastBacDual with LFn + FlaAAA
Plasmid#84867PurposeBaculovirus Expression of LFn-FlaAAA fusion. This is a negative control for LFn FlaA in which the 3 C-terminal leucines have been replaced by alanine.DepositorInsertsLFn
FlaAAA
UseBaculovirusTags6xHisExpressionInsectMutation3 C-terminal leucines have been replaced by alani…PromoterPolyhedrinAvailable sinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-KSENB-Nup133-mut
Plasmid#87339PurposeTo express human Nup133 in mammalian cells. It contains synonymous mutations that are not targeted by shRNADepositorInsertNUP133 (NUP133 Human)
UseTagsEGFPExpressionMammalianMutationshRNA resistant changes; deletion of Nup133 AA1-7PromoterCMVAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPPAT-2XStrep
Plasmid#125620Purposeexpresses human PPAT protein with a C-terminal 2XStrep affinity tagDepositorInsertPPAT (PPAT Human)
UseTags2X Strep-Tag IIExpressionMammalianMutationPromoterCMVAvailable sinceSept. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_SNORD27h
Plasmid#73066Purposeexpression clone for human SNORD27 (C/D box snoRNA U27)DepositorInsertSNORD27 (SNORD27 Human)
UseTagsno tagExpressionMammalianMutationexpression cassette for SNORD27PromoterCMVAvailable sinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
MigR1-AE9aP
Plasmid#12434DepositorUseRetroviralTagsHAExpressionMammalianMutationAML1-ETO9a cDNA as in t(8;21) patientsPromoterAvailable sinceJan. 4, 2007AvailabilityAcademic Institutions and Nonprofits only -
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pGEX-GP-2-DNMT3A PWWP
Plasmid#59696PurposeContains histone modification interacting domain (DNMT3A PWWP)DepositorInsertDNMT3A PWWP (Dnmt3a Mouse)
UseTagsExpressionBacterialMutationPromotertac promoterAvailable sinceSept. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEX-GP-2-CBX7 Chromo
Plasmid#59695PurposeContains histone modification interacting domain (CBX7 Chromo)DepositorInsertCBX7 Chromo (CBX7 Human)
UseTagsGSTExpressionBacterialMutationPromotertac promoterAvailable sinceSept. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pKI-cjFOXL2-coemiRFP670-PGK-Puro
Plasmid#186168PurposeKnock-in gene targeting in marmoset cells at FOXL2 locusDepositorInsertemiRFP670
UseMarmoset targetingTagsExpressionMutationhuman codon-optimizedPromoterAvailable sinceNov. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag4A-MITF-M (D380N)
Plasmid#31248DepositorInsertMITF-M (MITF Human)
UseTagsFLAGExpressionBacterial and MammalianMutationAspartic acid 380 changed to AsparaginePromoterAvailable sinceJuly 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSicoR-Ef1a-mCh-Puro-GFPi
Plasmid#31848DepositorInsertGFP RNAi
UseCre/Lox, Lentiviral, and RNAiTagsExpressionMammalianMutationPromoterU6 for shRNA; Ef1alpha for mCherry-puromycinAvailable sinceJan. 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pYPQ292 (AaCas12b)-D570A-TV-MS2-TV
Plasmid#136381PurposeCRISPRa Gateway entry clone for catalytically dead AaCas12b (D570A) fused with TV activation system, followed by T2A and MCP-TV fusion proteinDepositorInsertAaCas12b (D570A)-TV-T2A-MCP-TV
UseCRISPRTagsExpressionPlantMutationD570APromoterAvailable sinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
CFF-EGFP
Plasmid#86061PurposeA mammalian expression plasmid encoding fibrocystin mimentic clone CFF (CD8a luminal domain, fibrocystin transmembrane and cytosolic domains) with C-terminus EGFP.DepositorInsertFibrocystin (Pkhd1 Mouse)
UseTagsGFPExpressionMammalianMutationcontains luminal and transmembrane domains of CD8…PromoterCMVAvailable sinceMay 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-ERBB4 (E317K)
Plasmid#29528DepositorInsertERBB4 (ERBB4 Human)
UseTagsExpressionMammalianMutationchanged glutamic acid 317 to lysinePromoterAvailable sinceJuly 20, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBabe-TetCMV-puro-mVenus-PA-Rac1-T17N
Plasmid#22018DepositorInsertmVenus-PA-Rac1-T17N (RAC1 Avena sativa (oat), Human)
UseRetroviralTagsExpressionMammalianMutationRac1 starts at I4 and contains mutation T17N.PromoterAvailable sinceSept. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
pBabe-PI(WT)-Rac1(WT)-mCherry
Plasmid#87550PurposePI(WT): Photo-inhibitable/blue light sensitive Rac1DepositorInsertsUseTagsmCherryExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBabe-TetCMV-puro-mCerulean-PA-Rac1
Plasmid#22031DepositorInsertmCerulean-PA-Rac1 (RAC1 Avena sativa (oat), Human)
UseRetroviralTagsExpressionMammalianMutationRac1 starts at I4 and contains mutations Q61L, E9…PromoterAvailable sinceSept. 29, 2009AvailabilityAcademic Institutions and Nonprofits only