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Plasmid#218361PurposeEmpty backbone with tetR-PtetA expression system with two tetR operator sitesDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only
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pSLIK 3xFLAG-NUP37 neo
Plasmid#220361PurposeLentiviral expression vector for an inducible 3xFLAG-tagged NUP37DepositorInsertNUP37 (NUP37 Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
plentiCRISPR-v2 Puro KIAA0141/DELE1_sg2
Plasmid#218525PurposesgRNA targeting human KIAA0141/DELE1DepositorInsertDELE1 (DELE1 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMApra-Plac-SO
Plasmid#218358PurposeEmpty vector with lacIq-Plac expression systemDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMApra-Ptet1
Plasmid#218360PurposeEmpty backbone with tetR-PtetA expression system with single tetR operator siteDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMApra-Ptol
Plasmid#218359PurposeEmpty vector with XylS-Pm expression systemDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC18T-mini-Tn7T-hph-Ptet2
Plasmid#218368PurposeEmpty backbone for Tn7-site insertion with tetR-PtetA expression system with two tetR operator sitesDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMay 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC18T-mini-Tn7T-hph-Ptet1
Plasmid#218367PurposeEmpty backbone for Tn7-site insertion with tetR-PtetA expression system with single tetR operator siteDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMay 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC18T-mini-Tn7T-hph-Ptac
Plasmid#218364PurposeEmpty backbone for Tn7-site insertion with lacIq-Ptac expression systemDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMay 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC18T-mini-Tn7T-hph-Ptol
Plasmid#218369PurposeEmpty backbone for Tn7-site insertion with XylS-Pm expression systemDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMay 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMApra-Ptac
Plasmid#218357PurposeEmpty vector with lacIq-Ptac expression systemDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMay 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pBTK1056
Plasmid#209546PurposeBTK Type 4 antibiotic resistance cassette for Golden Gate assemblyDepositorInsertType 4 Kanamycin resistance cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 12, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
12x PRE TK luc
Plasmid#206163PurposeLuciferase reporter construct containing 12 consensus PGR binding sites upstream of a minimal TK promoter, derived from Addgene #11350DepositorInsert12X PRE TK luciferase
UseLuciferaseTagsExpressionMammalianMutationPromotermin TKAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
p2T-CMV-evoCjCas9-eAID-BlastR
Plasmid#194062PurposeMammalian expression of evoCjCas9 eAIDDepositorInsertevoCjCas9-eAID
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceSept. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pL0_EF-PtBle
Plasmid#202180PurposeLevel 0 plasmid containing a phleomycin antibiotic cassette (fcpF promoter) with EF overhangs used to build a level 1 construct.DepositorInsertShBle expression cassette
UseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 29, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pQIq_MRGS_HIS8_(27F3)_FLAG
Plasmid#199615PurposeEncodes DARPin-FLAG 27F3 for bacterial expression and column purificationDepositorInsertDARPin-FLAG 27F3
UseTags8xHis and FLAGExpressionBacterialMutationPromoterT5Available sinceMay 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
XIAP (pBS0852)
Plasmid#185282PurposeFor the mammalian expression of the human protein XIAP. This is the positive control we used in our apoptosis assay.DepositorInsertXIAP (XIAP Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMH003_phiC31_neo-5xTetO-pEF-H2B-mCitrine-coreHS4-pRSV-H2B-mCherry
Plasmid#179431PurposeDual fluorescent reporter construct with single core HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, phiC31 attB for integrationDepositorInsertTRE-cit-coreHS4-mch (SC)
UseTagsExpressionMammalianMutationPromoterpEF alpha, pRSVAvailable sinceMay 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV Cbx1-V5
Plasmid#175106PurposeLentiviral expression of mouse Cbx1-V5DepositorInsertCbx1 (Cbx1 Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC6A14
Plasmid#131865PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC6A14 (SLC6A14 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 4, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTCF158 Human beta-2 microglobulin
Plasmid#180457PurposeE. coli expression of class I MHC light chainDepositorInsertHuman beta-2 microglobulin
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-SH3BP4
Plasmid#46891Purposeexpresses HA tagged SH3BP4 in mammalian cellsDepositorInsertSH3BP4 (SH3BP4 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceSept. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
sgOpti
Plasmid#85681PurposeLentiviral vector to express sgRNAs. The cloning site is BsmBI.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-ITR (AAV-KPL)
Plasmid#60224PurposeExpresses Cre recombinase and Renilla luciferase from the EFS promoter and three U6-driven sgRNAs targeting Kras, p53, and Lkb1. Contains KrasG12D HDR donor. AAV backbone.DepositorInsertsUseAAV, CRISPR, Cre/Lox, and Mouse TargetingTagsCre-HA and Rluc-P2AExpressionMammalianMutationPromoterEFS, No promoter, and U6Available sinceOct. 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
HA-RNF4/pRK5
Plasmid#59743PurposeExpresses RNF4 in mammalian cellsDepositorInsertRNF4 (RNF4 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pmEGFP_a_tubulin_C1
Plasmid#21039DepositorInsertalpha Tubulin (TUBA1B Human)
UseTagspmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 11, 2009AvailabilityAcademic Institutions and Nonprofits only -
v3em-Cterm-PEmaxdeltaRNaseH-dualU6-Dnmt1-PE3-loxP
Plasmid#207862PurposeAAV genome encoding C-terminal PEmaxDRNaseH and U6 expression cassettes for Dnmt1 PE3 pegRNA and ngRNADepositorInsertNpuC-Cterm-PEmaxDRNaseH-dualU6-Dnmt1-PE3-loxP
UseAAVTagsExpressionMutationSee ManuscriptPromoterCbhAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBAD-mLemon
Plasmid#191256PurposeBacterial expression plasmids for pH sensitive fluorescent protein mLemonDepositorInsertmLemon
UseTagsHis tagExpressionBacterialMutationPromoterAvailable sinceNov. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSBbi G418R mbIL21
Plasmid#194329PurposeConsitiutive mammalian expression of membrane bound human IL-21DepositorInsertmbIL-21 (IL21 Human)
UseTagsExpressionMammalianMutationPromoterEF1-aAvailable sinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only