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HA-GABARAPL2

Plasmid

#137761

Purpose

Expression of GABARAPL2

Depositor

Carol Mercer

Article

Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

Insert

GABARAPL2 (GABARAPL2 Human)

Use

Tags

HA and myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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VRK1_HUMAN_D0

Plasmid

#79684

Purpose

This plasmid encodes the kinase domain of VRK1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

Markus Seeliger, Nicholas Levinson, John Chodera

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

VRK1 (VRK1 Human)

Use

Tags

His10-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
dCas9-DNMT3a-DNMT3l-3xFLAG-tagBFP

Plasmid

#154139

Purpose

Expresses the dCas9-DNMT3a-DNMT3l-3xFLAG-tagBFP fusion protein for targeted DNA methylation.

Depositor

Albert Jeltsch

Article

Hofacker et al Int J Mol Sci. 2020 Jan 13;21(2). pii: i

Insert

dCas9, DNMT3a (catalytic domain), DNMT3l (C-terminal part), tagBFP

Use

Tags

3x FLAG and tagBFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR_P2R-P3_R2-miniTurbo-mVenus-STOP-L3

Plasmid

#127356

Purpose

gateway entry vector for making C-terminal miniTurbo-mVenus fusion with nuclear and non-nuclear proteins

Depositor

Dominique Bergmann

Article

Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

Insert

miniTurbo (BirA mutant)

Use

Gateway entry vector

Tags

GS linker, V5, and mVenus

Expression

Mutation

aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

Promoter

no promoter

Available since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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CYFIP1.mCherry

Plasmid

#122051

Purpose

Expresses human CYFIP1 in mammalian cells.

Depositor

Josef Kittler

Article

Pathania et al Transl Psychiatry. 2014 Mar 25;4:e374. d

Insert

CYFIP1 (CYFIP1 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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EGFR-ET1

Plasmid

#44185

Depositor

Timothy Springer

Article

Mi et al Biochemistry. 2008 Sep 30;47(39):10314-2

Insert

EGFR-ET1 (EGFR Human)

Use

Tags

3C cleavge site, protein C peptide tag, his6, SBP…

Expression

Mammalian

Mutation

I104V and D254N relative to NP_005219.2

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pENTR1a-tagRFP-KDEL

Plasmid

#114177

Purpose

Gateway entry clone containing tagRFP with a signal peptide and KDEL ER retention signal for expression of tagRFP in the ER

Depositor

Nicolas Demaurex

Article

Nunes-Hasler et al Nat Commun. 2017 Nov 24;8(1):1852. doi:

Insert

tagRFP-KDEL

Use

Gateway entry clone

Tags

KDEL retention signal and signal peptide

Expression

Mutation

Promoter

Available since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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phage UbiC NLS HA stdMCP Halo

Plasmid

#104098

Purpose

Lentiviral vector expressing synonymous tandem MS2 coat protein fused to Halo. Used to label reporter mRNAs.

Depositor

Jeffrey Chao

Article

Horvathova et al Mol Cell. 2017 Nov 2;68(3):615-625.e9. d

Insert

stdMCP-Halo

Use

Lentiviral

Tags

C-terminal Halo-tag, N-terminal NLS, and Two syno…

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pYPQ166-SpRY

Plasmid

#161520

Purpose

Gateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zSpRYCas9-NLS without promoter

Depositor

Yiping Qi

Article

Ren et al Nat Plants. 2021 Jan 4. pii: 10.1038/s41

Insert

zSpRYCas9

Use

CRISPR; Gateway compatible zsprycas9 entry clone

Tags

3X FLAG, NLS and NLS

Expression

Plant

Mutation

Promoter

Available since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pAc-His-hAR-2

Plasmid

#89091

Purpose

baculovirus transfer vector for histidine-tagged full-length human androgen receptor, starts Met-Ala-His6-Met-hAR

Depositor

Elizabeth Wilson

Article

Liao et al Biochemistry. 1999 Jul 27;38(30):9718-27

Insert

hAR (AR Human)

Use

Tags

His

Expression

Mammalian

Mutation

Promoter

Available since

April 19, 2017

Availability

Academic Institutions and Nonprofits only