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pcDNA3.1-mhYFP

Plasmid

#186533

Purpose

Monomeric hyperfolder YFP fluorescent protein: cytosolic expression in mammalian cells

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

Monomeric hyperfolder YFP

Use

Tags

Expression

Mammalian

Mutation

hfYFP-S147P/L195M/V206K

Promoter

CMV

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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mCherry-Cytb5

Plasmid

#182579

Purpose

Expresses mCherry targeted to the ER via the CytB5RR tail anchor sequence

Depositor

Uri Manor

Article

Schiavon et al Nat Methods. 2020 Sep;17(9):917-921. doi

Insert

mCherry-Cytb5

Use

Tags

Cytb5

Expression

Mammalian

Mutation

Promoter

Available since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-CMV-Luc2-IRES-ZsGreen-W

Plasmid

#164432

Purpose

Lentiviral vector backbone encoding firefly luciferase and ZsGreen

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

Inserts

Luciferase
ZsGreen

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pmCherry Paxillin

Plasmid

#50526

Depositor

Kenneth Yamada

Insert

PXN paxillin (PXN Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pFB1_hPMS2

Plasmid

#129425

Purpose

Expression human PMS2 protein using baculovirus

Depositor

Peggy Hsieh

Article

Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

Insert

human PMS2 (PMS2 Human)

Use

Tags

Expression

Insect

Mutation

Promoter

Polyhedrin promoter

Available since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1alpha-nCoV2019-N-meGFP-IRES-Puro

Plasmid

#185451

Purpose

Expresses SARS-CoV-2 nucleocapsid protein with a c-terminal monomeric EGFP in mammalian cells

Depositor

Ahmet Yildiz

Article

Jack et al PLoS Biol. 2021 Oct 11;19(10):e3001425.

Insert

Nucleocapsid (N SARS-CoV-2)

Use

Lentiviral

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

EF1alpha

Available since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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BSG-bio-His

Plasmid

#53146

Purpose

Expresses full-length Basigin ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

Basigin (BSG Human)

Use

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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AiP13038 - pAAV-AiE0743m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3038)

Plasmid

#191720

Purpose

AI plasmid ALIAS: AiP13038. Expression of SYFP2 (yellow fluorescent protein) in striatal and lateral septum cholinergic neurons

Depositor

The Allen Institute for Brain Science, Jonathan Ting

Has Service

AAV PHP.eB

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

none

Promoter

Available since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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AiP2105 - pAAV-AiE2638m-minBG-SYFP2-WPRE3-BGHpA

Plasmid

#230551

Purpose

AiE2638m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

The Allen Institute for Brain Science, Bosiljka Tasic

Article

Ben-Simon et al bioRxiv [Preprint]. 2024 Sep 26:2024.06.

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

Promoter

minBG

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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Col1A2:TFPnls-T2a-mErt-Cre-Ert

Plasmid

#111151

Purpose

Fibroblasts specific inducible Cre

Depositor

Elly Tanaka

Insert

TFPnls-T2a-mErt-Cre-Ert

Use

Cre/Lox

Tags

Expression

Mutation

wt

Promoter

Col1A2

Available since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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pGRG25

Plasmid

#16665

Depositor

Nancy Craig

Article

McKenzie et al BMC Microbiol. 2006 . 6():39.

Insert

mTn7::MCS

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 10, 2008

Availability

Academic Institutions and Nonprofits only

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pGBKT7-GW

Plasmid

#61703

Purpose

Gateway compatible Y2H vector - BD

Depositor

Yuhai Cui

Article

Lu et al Plant J. 2010 Jan;61(2):259-70. doi: 10.

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 4, 2015

Availability

Academic Institutions and Nonprofits only

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pHAGE-FGFR1

Plasmid

#116740

Purpose

Lentiviral expression of FGFR1

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

FGFR1 (FGFR1 Human)

Use

Lentiviral

Tags

Expression

Mutation

WT

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3.3_CoV2_B.1.1.7

Plasmid

#170451

Purpose

Express SARS-CoV-2 B.1.1.7 (UK strain / alpha strain) spike protein with an 18aa deletion on the C-terminal tail. Used for SARS2 (UK strain) pseudotyped virus production.

Depositor

David Nemazee

Article

Yuan et al Science. 2021 Aug 13;373(6556):818-823.

Insert

SARS-CoV-2 501V2 spike D18 (S SARS-CoV-2, UK strain (B.1.1.7))

Use

Tags

NA

Expression

Mammalian

Mutation

HY69-70del, Y144del, N501Y, A570D, D614G, P681H, …

Promoter

CMV

Available since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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CRF1R-GFP

Plasmid

#208912

Purpose

encodes the human corticotropin releasing factor 1 receptors, tagged with YFP

Depositor

Christian Gruber

Article

Fahradpour et al Front Pharmacol. 2017 Sep 25;8:616. doi:

Insert

CRHR1 (CRHR1 Human)

Use

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCRISPR-PsppA-mCherry

Plasmid

#225428

Purpose

Plasmid encodes SgRNA, SpCas9 and homologous arms for homologous recombination of mCherry

Depositor

Geir Mathiesen

Article

Wiull et al Microbiol Spectr. 2025 Feb 4;13(2):e0202

Insert

mCherry, Cas9, SgRNA

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

PsppA upstream of mCherry and Cas9, P3 upstream o…

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pSNAPf-N1

Plasmid

#58187

Depositor

Michael Davidson

Type

Empty backbone

Use

Tags

SNAP tag

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pmScarlet-HSP70

Plasmid

#163790

Purpose

Expresses mScarlet-Hsp70 in mammalian cells

Depositor

Vincent Timmerman

Article

Adriaenssens et al Sci Rep. 2020 May 29;10(1):8755. doi: 10

Insert

HSP70 (HSPA1A Human)

Use

Tags

mScarlet

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti X2 Blast DEST (w46-2)

Plasmid

#17391

Purpose

3rd gen lentiviral Gateway destination vector, RNAi, 3’LTR insertion, Blast

Depositor

Paul Kaufman, Eric Campeau

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Lentiviral and RNAi; Destination vector

Tags

Expression

Mutation

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pLNCX2-mCherry-CHMP4B

Plasmid

#116923

Purpose

expresses mCherry-CHMP4B in mammalian cells

Depositor

Sanford Simon

Article

Bleck et al Proc Natl Acad Sci U S A. 2014 Aug 19;11

Insert

CHMP4B (CHMP4B Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 24, 2018

Availability

Academic Institutions and Nonprofits only

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