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Plasmid#91194PurposeWDV replicon T-DNA for gene targeting in wheat scutella, H840A single nickase (nTaCas9_H840A+gUbi8+donor)DepositorInsertnTaCas9_H840A+gUbi8+donor
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_C3306
Plasmid#91102PurposeModule C, Promoter: FMV 34S, Gene: TALE-VP64 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: HSPDepositorInsertTALE-VP64 backbone with Esp3I ccdb cassette for repeat cloning
UseTALENTagsExpressionMutationPromoterFMV 34SAvailable sinceJan. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pGEX-4T-1-Shp2 SH2s
Plasmid#67575PurposeExpresses GST-tagged Shp2 SH2 domains in bacteriaDepositorInsertSrc homology 2 domain-containing phosphatase 2 SH2s
UseTagsGSTExpressionBacterialMutationDeleted amino acids 219-597PromoterAvailable sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pME-2PH-PLCd
Plasmid#71283PurposeMultisite gateway entry clone for adding C-terminal fusions of human 2PH-PLC-deltaDepositorInsert2PH-PLC delta (Human)
UseGateway multisite middle entry cloneTagsExpressionMutationPromoterAvailable sinceDec. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
CSII-IDR2-chimpTRIM5-FOS
Plasmid#79065PurposeExpress chimpanzee TRIM5alpha in mammalan cellsDepositorInsertTRIM5alpha(chimpanzee)-FOS
UseTagsOneSTrEP-FLAGExpressionMammalianMutationPromoterCMVAvailable sinceMarch 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSTAP-seq_fly-tj
Plasmid#86386PurposeVector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.DepositorInsertD. melanogaster tj enhancer
UseStap-seq screening vectorTagsExpressionMutationPromoterAvailable sinceJune 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyn-FRB-Venus-173-N
Plasmid#87615PurposeFRB-tagged N-terminal Venus (YFP) fragment for BiFC; localizes to plasma membrane.DepositorInsertLyn-FRB-Venus173-N
UseTagsN-terminal targeting sequence from Lyn kinaseExpressionMammalianMutationPromoterCMVAvailable sinceMay 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCRII-Plxnb3-ISH-2kb
Plasmid#68037PurposeContains 2kb cDNA fragment for probe synthesis for mRNA in situ hybridization, species: mouseDepositorInsertPlexin-B3 (Plxnb3 Mouse)
UseMrna in situ hybridization probeTagsExpressionMutationPromoterAvailable sinceAug. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
hSTARR-seq_SULT1C vector
Plasmid#99294PurposeVector to measure enhancer activity of candidates from a DNA library by STARR-seq.DepositorTypeEmpty backboneUseHstarr-seq screening vectorTagsExpressionMammalianMutationPromoterAvailable sinceSept. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
hSTARR-seq_TTF2 vector
Plasmid#99293PurposeVector to measure enhancer activity of candidates from a DNA library by STARR-seq.DepositorTypeEmpty backboneUseHstarr-seq screening vectorTagsExpressionMammalianMutationPromoterAvailable sinceSept. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A1010
Plasmid#91030PurposeModule A, Promoter: ZmUbi, Gene: TALEN_1 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: none (ends with P2A to be fused to TALEN_2)DepositorInsertTALEN_1 backbone with Esp3I ccdb cassette for repeat cloning
UseTALENTagsExpressionMutationPromoterZmUbiAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A1002
Plasmid#91029PurposeModule A, Promoter: AtUbi10, Gene: TALEN_1 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: none (ends with P2A to be fused to TALEN_2)DepositorInsertTALEN_1 backbone with Esp3I ccdb cassette for repeat cloning
UseTALENTagsExpressionMutationPromoterAtUbi10Available sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTaCas9-H840A_5
Plasmid#91172PurposeCas9 only expression, Cas9 Type: TaCas9_H840A (nickase), Plant Selection: PvUbi2:hpt IIDepositorInsertTaCas9_H840A (nickase)
UseCRISPRTagsExpressionPlantMutationH840APromoterAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTaCas9-D10A_6
Plasmid#91171PurposeCas9 only expression, Cas9 Type: TaCas9_D10A (nickase), Plant Selection: PvUbi2:barDepositorInsertTaCas9_D10A (nickase)
UseCRISPRTagsExpressionPlantMutationD10APromoterAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAtCas9-D10A_3
Plasmid#91161PurposeCas9 only expression, Cas9 Type: AtCas9_D10A (nickase), Plant Selection: 2x35S:barDepositorInsertAtCas9_D10A (nickase)
UseCRISPRTagsExpressionPlantMutationD10APromoterAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A1410
Plasmid#91035PurposeModule A, Promoter: ZmUbi, Gene: TaCas9_dead (D10A + H840A), Terminator: HSPDepositorInsertTaCas9_dead (D10A + H840A)
UseCRISPRTagsExpressionMutationD10A, H840APromoterZmUbiAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTC440
Plasmid#91225Purposeprotoplast vector expressing gRNA38 targeting barley MLO (control without TREX2 expression)DepositorInsertgRNA targeting barley MLO
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTC441
Plasmid#91218Purposeprotoplast vector for targeted deletion of MLO gene in barley (PvUbi1 promoter driving the gRNA array)DepositorInsertgRNAs targeting MLO gene in barley
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only