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PaADA

Plasmid

#250880

Purpose

Pseudomonas aeruginosa adenine deaminase (PaADA; Pa0148; UniProt Q9I6Y4)

Depositor

Vern Schramm

Article

Gilaj et al Anal Biochem. 2026 Jul;714:116109. doi:

Insert

Pseudomonas aeruginosa adenine deaminase (PaADA; UniProt Q9I6Y4) (PA0148 )

Tags

6xHis-TEV-

Expression

Bacterial

Available Since

March 16, 2026

Availability

Academic Institutions and Nonprofits only

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pTW335l

Plasmid

#246590

Purpose

G. hirsutum Rubisco gene

Depositor

Tina Wang

Article

Li et al Proc Natl Acad Sci U S A. 2025 Sep 16;12

Insert

G. hirsutum RbcL, RbcS 6xhis (rbcL G. hirsutum RbcL)

Expression

Bacterial

Available Since

Oct. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pJMH024

Plasmid

#186636

Purpose

aadA.co between homology arms for pilA

Depositor

Adam Arkin

Article

Hilzinger et al Biotechnol Bioeng. 2025 Jan;122(1):44-52

Insert

aadA.co

Use

Synthetic Biology

Expression

Bacterial

Available Since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-CBS (H65R)

Plasmid

#182924

Purpose

Mammalian expression plasmid for human CBS mutant H65R

Depositor

Philip Eaton

Article

Switzer et al Proc Natl Acad Sci U S A. 2023 Jan 17;12

Insert

Cystathionine Beta-Synthase (CBS Human)

Tags

eGFP

Expression

Mammalian

Mutation

changed histidine 65 to arginine

Promoter

CMV

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pRS314-PRP16

Plasmid

#89971

Purpose

Prp16 in a trp backbone complements prp16 genomic deletion

Depositor

Christine Guthrie

Article

Mayerle et al RNA. 2016 May;22(5):793-809. doi: 10.126

Insert

PRP16 (PRP16 Budding Yeast)

Expression

Yeast

Mutation

none

Available Since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pUC-UAS1B5-Leum

Plasmid

#44313

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B5-Leum promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B5-Leum

Available Since

July 15, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pET15b-At 14-3-3 iota

Plasmid

#44937

Depositor

Robert Ferl

Article

Sehnke et al Plant Physiol. 2006 Feb;140(2):647-60. E

Insert

14-3-3 iota (GRF12 Mustard Weed)

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

May 14, 2013

Availability

Academic Institutions and Nonprofits only
pUC-UAS1B8-TEF(203)

Plasmid

#44378

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B8-TEF(203) promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B8-TEF(203)

Available Since

April 18, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pUC-UAS1B6-Leum

Plasmid

#44314

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B6-Leum promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B6-Leum

Available Since

April 18, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pUC-UAS1B10-Leum

Plasmid

#44318

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B10-Leum promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B10-Leum

Available Since

April 18, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pUC-UAS1B8-TEF(804)

Plasmid

#44368

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B8-TEF(804) promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B8-TEF(804)

Available Since

April 18, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pUC-Leum

Plasmid

#44364

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

Leum promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

N/A

Promoter

Leum promoter

Available Since

April 18, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pUC-UAS1B12-Leum

Plasmid

#44320

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B12-Leum promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B12-Leum

Available Since

April 17, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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myc-POMP

Plasmid

#86764

Purpose

N-terminal myc-tagged protein expression in mammalian cells

Depositor

Do-Hyung Kim

Article

Yun et al Mol Cell. 2016 Feb 18;61(4):625-39. doi:

Insert

human POMP (POMP Human)

Tags

myc

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-nAChr-Alpha6

Plasmid

#48812

Purpose

this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF

Depositor

Henry Lester

Article

Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

Insert

nAChr-alpha6 (Chrna6 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

April 27, 2016

Availability

Academic Institutions and Nonprofits only

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10279-M28-663

Plasmid

#225662

Purpose

Lentivirus protein expression. Matched control construct for 8522-M01-663 (Addgene # 225660) gives basal expression from mCMVp for gating/thresholding

Depositor

Dominic Esposito

Article

Tang et al Stem Cell Reports. 2015 Jan 13;4(1):155-

Insert

dsCopGFP

Use

Lentiviral

Promoter

mCMVp

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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Negative Control Binder

Plasmid

#179140

Purpose

Binder: SspB that carries two point mutations reducing homodimerization(Y11K/A15E, residue numbering from pdb: 1ou9) and an additionional A70Q mutation greatly reduces affinity for SsrA peptide

Depositor

Klaus Hahn

Article

Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.

Insert

SspB

Tags

FLAG and mCherry

Expression

Mammalian

Mutation

Y11K/A15E, residue numbering from pdb: 1ou9. Addi…

Available Since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pVenusCfC

Plasmid

#128567

Purpose

Protein interaction screening tool with Gateway-compatible BiFC vectors comprising of a set of 4 vectors permiting N or C fusions of candidate genes to the N or C fragment of Split-Venus.

Depositor

Oliver Vugrek

Article

Lepur et al J Biomol Screen. 2016 Dec;21(10):1100-11

Insert

Venus

Expression

Mammalian

Available Since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pVenusCfN

Plasmid

#128566

Purpose

Protein interaction screening tool with Gateway-compatible BiFC vectors comprising of a set of 4 vectors permiting N or C fusions of candidate genes to the N or C fragment of Split-Venus.

Depositor

Oliver Vugrek

Article

Lepur et al J Biomol Screen. 2016 Dec;21(10):1100-11

Insert

Venus

Expression

Mammalian

Available Since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-AID-Ns3h-UGI-T2A-mTagBFP2

Plasmid

#221223

Purpose

Express HACE Editor as a fusion of AID cytidine deaminase and Ns3h helicase with mTagBFP2 reporter

Depositor

Fei Chen

Article

Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

Insert

AID-Ns3h-UGI-T2A-mTagBFP2

Expression

Mammalian

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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