Showing: 6621 - 6640 of 18082 results
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Plasmid#71666PurposeExpression of human codon-optimized dCas9-DNMT3A fusion with T2A-EGFP for targeted DNA methylation in mammalian cells; cloning backbone for sgRNADepositorInsertS. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP (DNMT3A Human, Synthetic, S. pyogenes)
UseCRISPRTags3xFLAG, SV40 NLS, and T2A-EGFPExpressionMammalianMutationD10A and H840A in S.pyogenes Cas9PromoterCBhAvailabilityAcademic Institutions and Nonprofits only -
pdCas9-DNMT3A-PuroR
Plasmid#71667PurposeExpression of human codon-optimized dCas9-DNMT3A fusion with T2A-PuroR for targeted DNA methylation in mammalian cells; cloning backbone for sgRNADepositorInsertS. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (DNMT3A Human, Synthetic, S. pyogenes)
UseCRISPRTags3xFLAG, SV40 NLS, and T2A-PuroRExpressionMammalianMutationD10A and H840A in S.pyogenes Cas9PromoterCBhAvailabilityAcademic Institutions and Nonprofits only -
pdCas9-DNMT3A-PuroR (ANV)
Plasmid#71684PurposeExpression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR; cloning backbone for sgRNADepositorInsertS. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (DNMT3A Human, Synthetic, S. pyogenes)
UseCRISPRTags3xFLAG, SV40 NLS, and T2A-PuroRExpressionMammalianMutationD10A and H840A in S.pyogenes Cas9; E756A inactiva…PromoterCBhAvailabilityAcademic Institutions and Nonprofits only -
pdCas9-DNMT3A-EGFP (ANV)
Plasmid#71685PurposeExpression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-EGFP; cloning backbone for sgRNADepositorInsertS. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP (DNMT3A Human, Synthetic, S. pyogenes)
UseCRISPRTags3xFLAG, SV40 NLS, and T2A-EGFPExpressionMammalianMutationD10A and H840A in S.pyogenes Cas9; E756A inactiva…PromoterCBhAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+ SMN1 myc HIS
Plasmid#71687PurposeMammalian expression of SMN1DepositorInsertSMN1 (SMN1 Human)
UseTagsHIS and mycExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pX330-U6-Chimeric_BB-CBh-hSpCas9-hGem(1/110)
Plasmid#71707PurposeA human codon-optimized SpCas9 fused to first 110 amino acids of human GEMININ and chimeric guide RNA expression plasmidDepositorInserthumanized S. pyogenes Cas9 fused to human Geminin (GMNN Human)
UseCRISPRTags3xFLAG and hGEM(1/110)ExpressionMammalianMutationPromoterCBhAvailabilityAcademic Institutions and Nonprofits only -
pCDH-CB1-HIF2a-GFP-T2A-Puro
Plasmid#71708PurposeLentiviral expression of HIF2A-GFPDepositorInsertHIF2A (EPAS1 Human)
UseLentiviralTagsEGFP and HAExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
alpha catenin conformation sensor
Plasmid#71709PurposeThis alpha catenin biosensor undergoes a change in FRET upon mechanical perturbation of the alpha catenin conformation.DepositorInsertalpha-catenin FRET Sensor
UseTagsECFP and YPETExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pET28a-MSP1D1delltaH4
Plasmid#71713PurposeMembrane scaffold protein (MSP), helix 4 deletionDepositorInsertApolipoprotein A1 (APOA1 Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pET28a-MSP1D1delltaH5
Plasmid#71714PurposeMembrane scaffold protein (MSP), helix 5 deletionDepositorInsertApolipoprotein A1 (APOA1 Human)
UseTags6xHis and TEV siteExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pET28a-MSP1D1deltaH4H5
Plasmid#71716PurposeMembrane scaffold protein (MSP), helix 4+ helix 5 deletionDepositorInsertApolipoprotein A1 (APOA1 Human)
UseTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pET28a-MSP1D1deltaH4-H6
Plasmid#71717PurposeMembrane scaffold protein (MSP), helix 4 to 6 deletionDepositorInsertApolipoprotein A1 (APOA1 Human)
UseTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pLZ29 (pCFJ151_Peft-3_degron_EmGFP_unc-54 3'UTR)
Plasmid#71719PurposeExpressing a GFP-tagged degron in the soma of C. elegansDepositorInsertauxin-responsive protein IAA17 (AXR3 Mustard Weed)
UseTagsEmGFPExpressionWormMutationminimal degron sequence (71-114aa) with start cod…Promotereef-1A.1 (eft-3)AvailabilityAcademic Institutions and Nonprofits only -
pLZ31 (pCFJ151_Peft-3_TIR1_linker_mRuby_unc-54 3'UTR)
Plasmid#71720PurposeExpressing an mRuby-tagged TIR1 in the soma of C. elegansDepositorInsertTRANSPORT INHIBITOR RESPONSE 1 (TIR1 Mustard Weed)
UseTagsmRubyExpressionWormMutationcodon-optimized for expression in C. elegans; Two…Promotereef-1A.1 (eft-3)AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-hHSF1 WT
Plasmid#71724Purposeplasmid expressing myc-his tag hHSF1 WTDepositorInsertHSF1 (HSF1 Human)
UseTagsMYC 6xHISExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
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pG1AK-sfGFP
Plasmid#71739PurposeModular shuttle vector for Geobacillus and E. coliDepositorInsertsfGFP
UseSynthetic Biology; Shuttle vectorTagsExpressionBacterialMutationPromoterpRplsWTAvailabilityAcademic Institutions and Nonprofits only -
pG1K
Plasmid#71741PurposeModular shuttle vector for Geobacillus and E. coliDepositorTypeEmpty backboneUseSynthetic Biology; Shuttle vectorTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pBSDONR P1-P4
Plasmid#71744Purposeampicillin resistant derivative of pDONR221 P1-P4 for multisite GATEWAY cloningDepositorTypeEmpty backboneUseGateway entry vector for use in multisite gateway…TagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pTA7001-DEST
Plasmid#71745PurposeGATEWAY compatible derivative of plant binary vector pTA7001 from Nam Chua's laboratory at The Rockefeller University, Dexamethasone inducibleDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterGAL4AvailabilityAcademic Institutions and Nonprofits only
Showing: 6621 - 6640 of 18082 results