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Plasmid#207837PurposeInducible hGRAD Plasmids for rapid depletion of GFP-tagged proteins in mammalian cellsDepositorInsertsUseTagsExpressionMammalianMutation215 amino acids of the full length proteinPromoterTRE3G BI promoterAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only
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pSAM2_mCherry_Mesp1
Plasmid#72687PurposeDoxycyline regulated expression of Mesp1 in mammalian cellsDepositorInsertMesp 1 (MESP1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMV-DoxycyclineAvailable sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
EGFP-HsBIN1_tv8
Plasmid#176012PurposeMammalian expression of Human EGFP-Bin1 (transcriptional variant 8, muscle specific). Parton lab clone HBHDepositorInsertBin1
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
H3S10ph biosensor
Plasmid#120809PurposeFRET biosensor. To monitor histone H3 Serine 10 phosphorylation in mammalian cells by FRETDepositorInsertMouse histone H3-CFP-FHA2-histone H3 peptide (1-14aa)-YFP
UseTagsExpressionMammalianMutationThreonine 3, Threonine 6, and Threonine 11 are mu…PromoterCMVAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLX307/PTPN11
Plasmid#140941Purposeexpresses PTPN11DepositorInsertPTPN11 protein tyrosine phosphatase non-receptor type 11 [ Homo sapiens (human) ] (PTPN11 Human)
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
Lenti-117G-hyeA3A-BE4max
Plasmid#157946PurposeLentiviral expression of hyeA3A-BE4maxDepositorInsertLenti -117G-hyeA3A-BE4max
UseCRISPR and LentiviralTagsExpressionMammalianMutationN57GPromoterAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSAM2_mCherry_Isl1
Plasmid#72696PurposeDoxycyline regulated expression of Isl1 in mammalian cellsDepositorInsertIslet1 (Isl1 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterCMV-DoxycyclineAvailable sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
W118-1_hZBTB7B
Plasmid#180380Purposelentiviral transduction of human ZBTB7B geneDepositorInsertZBTB7B (ZBTB7B Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSAM2_mCherry_Mesp2
Plasmid#72693PurposeDoxycyline regulated expression of Mesp2 in mammalian cellsDepositorInsertMesp 2 (Mesp2 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterCMV-DoxycyclineAvailable sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
human SLC12A5-V5-HA-2A-Luciferase repair template
Plasmid#158576PurposeFor targeted insertion of V5-HA-2A-Luciferase reporter before the stop codon of the human SLC12A5 gene that encodes neuronal chloride transporter KCC2 protein.DepositorInserthuman SLC12A5 5' homology arm-V5-HA-2A-Luciferase-3' homology arm (SLC12A5 Human)
UseTagsV5 and HA on the endogenous KCC2 protein productExpressionMammalianMutationPromoterAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pME-GcAMP5
Plasmid#126569PurposeMultisite gateway middle entry clone containing zebrafish calcium reporter GcAMP5. Parton lab clone ANR.DepositorInsertGcAMP5
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceNov. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
actc1b-GcAMP5
Plasmid#126563PurposeMuscle specific expression of the calcium reporter GcAMP5 in zebrafish. Parton lab clone APTDepositorInsertGcAMP5
UseTagsExpressionMutationPromoterAvailable sinceNov. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Bin1a
Plasmid#176013PurposeMammalian expression of zebrafish EGFP-Bin1a. Parton lab clone HYADepositorInsertBin1a
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-SLC39A11_STOP
Plasmid#161307PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertSLC39A11 (SLC39A11 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 15, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLX307/PTPN11 T73I
Plasmid#140939Purposeexpresses PTPN11 T73IDepositorInsertPTPN11 protein tyrosine phosphatase non-receptor type 11 [ Homo sapiens (human) ] (PTPN11 Human)
UseTagsV5ExpressionMammalianMutationT73IPromoterEF1aAvailable sinceJune 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
W118-1_hRSK3(RPS6KA2)
Plasmid#180384Purposelentiviral transduction of human RSK3 (RPS6KA2) geneDepositorInsertRPS6KA2 (RPS6KA2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Bin1b
Plasmid#176014PurposeMammalian expression of zebrafish EGFP-Bin1b. Parton lab clone HYGDepositorInsertBin1b (bin1b Zebrafish)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-nAChr-beta3-YFP
Plasmid#50484Purposethis report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRFDepositorInsertnAChRBeta3 subunit, isoform 1 (Chrnb3 Mouse)
UseTagsYFPExpressionMammalianMutationYFP fusion in M3-M4 loop (after residue P379). O…PromoterCMVAvailable sinceJan. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLX307/PTPN11 E76G
Plasmid#140862Purposeexpresses PTPN11 E76GDepositorInsertPTPN11 protein tyrosine phosphatase non-receptor type 11 [ Homo sapiens (human) ] (PTPN11 Human)
UseTagsExpressionMammalianMutationE76GPromoterEF1aAvailable sinceJune 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUAST-HA, Pbl-A PH+DH domains
Plasmid#69793PurposePebble isoform A, PH and DH domains, in P element-based pUAST vector for Gal4-regulated expression in Drosophila.DepositorInsertPebble DH +PH domains (pbl Fly)
UseP element-based puast vector for gal4-regulated e…TagsHA tagExpressionInsectMutationPbl-A (NP_729306.1) amino acids 385-647.Promoterhsp70 promoterAvailable sinceNov. 24, 2015AvailabilityAcademic Institutions and Nonprofits only