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RP1B FUS 1-163

Plasmid

#127192

Purpose

Protein expression for affinity purification

Depositor

Nicolas Fawzi

Article

Murthy et al Nat Struct Mol Biol. 2019 Jul;26(7):637-

Insert

FUS 1-163 (FUS Human)

Use

Tags

hexahistidine

Expression

Bacterial

Mutation

Promoter

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV.Syn.Flex.NES-jRGECO1a.WPRE.SV40

Plasmid

#100853

Purpose

AAV expression of Cre recombinase-activated jRGECO1a, a red fluorescent calcium sensor protein, from the Synapsin promoter

Depositor

GENIE Project, Douglas Kim

Article

Dana et al Elife. 2016 Mar 24;5. pii: e12727. doi:

Has Service

AAV Retrograde and AAV1

Insert

jRGECO1a

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Synapsin

Available since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pYLsgRNA-AtU6-29

Plasmid

#66203

Purpose

cloning of sgRNAs for expression in plants

Depositor

Yao-Guang Liu

Article

Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

Type

Empty backbone

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Aug. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pRc/CMV HA TrkB

Plasmid

#39978

Depositor

Yves-Alain Barde

Article

Bibel et al EMBO J. 1999 Feb 1;18(3):616-22.

Insert

TrkB (Ntrk2 Rat)

Use

Tags

BM40 signal sequence and HA

Expression

Mammalian

Mutation

endogenous signal sequence was replaced with BM40…

Promoter

CMV

Available since

March 17, 2014

Availability

Academic Institutions and Nonprofits only
pJBJZ_006

Plasmid

#155359

Purpose

Zeomycin derivative of lambda-red pKD3 plasmid from Datsenko and Wanner (2000)

Depositor

Elisabeth Raleigh

Article

Zaworski et al PLoS Genet. 2022 Apr 4;18(4):e1009943. d

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Sept. 30, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1 EYFP H148Q/I152L

Plasmid

#25872

Depositor

Peter Haggie

Article

Galietta et al FEBS Lett. 2001 Jun 22. 499(3):220-4.

Insert

EYFP H148Q/I152L

Use

Tags

Expression

Mammalian

Mutation

EYFP from pEYFP (Clontech) with H148Q and I152L m…

Promoter

Available since

Aug. 16, 2010

Availability

Academic Institutions and Nonprofits only

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pLV-SI-121

Plasmid

#131126

Purpose

A-to-G base editing reporter

Depositor

Nozomu Yachie

Article

Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

Insert

mutant EGFP

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Sept. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pNIC28-MacroGreen

Plasmid

#160665

Purpose

Expresses GFP fusion of a multisite mutant of the Af1521 protein

Depositor

Herwig Schüler

Article

Garcia-Saura et al Commun Biol. 2021 Jul 28;4(1):919. doi:

Insert

Af1521 modified synthetic construct eGFP fusion

Use

Tags

eGFP and hexahistidine_TEV recognition

Expression

Bacterial

Mutation

Promoter

LacI

Available since

July 30, 2021

Availability

Academic Institutions and Nonprofits only

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pDsRed-attP

Plasmid

#51019

Purpose

Vector for generating dsDNA donors for homology-directed repair to replace genes or other genomic sequence with an attP docking site. Contains the visible marker 3xP3-DsRed. As known as pHD-DsRed-attP

Depositor

Melissa Harrison, Kate O'Connor-Giles, Jill Wildonger

Article

Gratz et al Genetics. 2014 Apr;196(4):961-71. doi: 1

Inserts

attP
LoxP-3xP3-DsRed-LoxP

Use

High copy, amp resistance

Tags

Expression

Mutation

Promoter

3xP3 and None

Available since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pBbA5c-MevT(CO)-MBIS(CO, ispA)

Plasmid

#35151

Depositor

Taek Soon Lee, Jay Keasling

Article

Peralta-Yahya et al Nat Commun. 2011 Sep 27;2:483. doi: 10.1

Insert

AtoB(co)-HMGS(co)-HMGR(co)-MK(co)-PMK(co)-PMD-Idi-IspA

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

R364S in Mevalonate Kinase

Promoter

LacUV5,

Available since

July 23, 2012

Availability

Academic Institutions and Nonprofits only

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pGWB402

Plasmid

#74796

Purpose

Gateway cloning compatible binary vector for expression of gene by CaMV35S promoter.

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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pRSII424

Plasmid

#35466

Depositor

Steven Haase

Article

Chee et al G3 (Bethesda). 2012 May;2(5):515-26. Epu

Type

Empty backbone

Use

Yeast 2 micron vector

Tags

Expression

Mutation

Promoter

None

Available since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only

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v3em-Cterm-PE6d-dualU6-Dnmt1-PE3-loxP

Plasmid

#207863

Purpose

AAV genome encoding C-terminal PE6d and U6 expression cassettes for Dnmt1 PE3 pegRNA and ngRNA

Depositor

David Liu

Article

Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

Insert

NpuC-Cterm-PE6d-dualU6-Dnmt1-PE3-loxP

Use

AAV

Tags

Expression

Mutation

See Manuscript

Promoter

Cbh

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pRRlsinPGK_H2BGFP_WPRE

Plasmid

#91788

Purpose

Constitutive expression of H2BGFP fusion using PGK promoter in a lentiviral backbone.

Depositor

Beverly Torok-Storb

Insert

Human histone H2B (H2BC11 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

PGK

Available since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

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eSpCas9(1.1)

Plasmid

#138555

Purpose

Expresses human codon-optimized enhanced specificity SpCas9 and blasticidin resistance: EFS promoter-eSpCas9(1.1)-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

Insert

eSpCas9(1.1)

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Mutation

K848A, K1003A, R1060A

Promoter

EFS

Available since

June 9, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-Rab27A

Plasmid

#89237

Purpose

Expression of GFP-tagged human Rab27A in mammalian cells

Depositor

William Gahl

Article

Westbroek et al Mol Genet Metab. 2008 Jun;94(2):248-54.

Insert

human Rab27A (RAB7A Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG_smFP HA

Plasmid

#59759

Purpose

encodes multiple copies of an epitope tag HA

Depositor

Loren Looger

Article

Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

Insert

smFP _HA

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 21, 2014

Availability

Academic Institutions and Nonprofits only

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N370S GBA pcDNA3.1

Plasmid

#188583

Purpose

Ectopic expression N370S GBA

Depositor

Anthony Schapira

Article

Smith et al Hum Mol Genet. 2022 Sep 20:ddac233. doi:

Insert

GBA (GBA1 Human)

Use

Tags

Expression

Mammalian

Mutation

N370S

Promoter

CMV

Available since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pRBC-sfGFP-150TAG

Plasmid

#174076

Purpose

Plasmid with p15a origin of replication expressing codon optimized superfolder GFP-150 TAG with C-terminal His6 tag, under T7 promoter,

Depositor

Ryan Mehl

Article

Zhu et al ACS Chem Biol. 2019 Jul 19;14(7):1564-15

Insert

Superfolder GFP

Use

Tags

His-6

Expression

Bacterial

Mutation

N150TAG

Promoter

T7

Available since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pYLsgRNA-AtU3d

Plasmid

#66200

Purpose

cloning of sgRNAs for expression in plants

Depositor

Yao-Guang Liu

Article

Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

Type

Empty backbone

Use

Cloning vector

Tags

Expression

Mutation

Promoter

AtU3d

Available since

Aug. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pLL3.7m-rsGreenF-E-Geminin(1-110)-2A-Dronpa-Cdt-IRES-H1-Skylan62A-2A-YFP-SLBP

Plasmid

#205762

Purpose

Single-color FUCCI4 senor for transitions of all four cell cylce phases

Depositor

Edward Boyden

Article

Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

Insert

rsGFE-Geminin(1-110)-2A-Dronpa-Cdt-IRES-H1-SK62A-2A-YFP-SLBP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

wt

Promoter

Available since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-hIFITM1

Plasmid

#58399

Purpose

Expressed human IFITM1 with an N-terminal HA tag in mammalian cells

Depositor

Howard Hang, Jacob Yount

Article

Yount et al Nat Chem Biol. 2010 Aug;6(8):610-4. doi:

Insert

IFITM1 (IFITM1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV IE

Available since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only

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Dual opposing T7 promoters reporter plasmid

Plasmid

#156457

Purpose

A mutational reporter plasmid that can gain Kanamycin resistance upon mutation. Contains two T7 promoters flanking the kanamycin resistance gene.

Depositor

Matthew D. Shoulders

Article

Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560

Insert

NeoR/KanR

Use

Tags

Expression

Bacterial

Mutation

ATG start codon mutated to ACG

Promoter

Em7

Available since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pTRIPZ

Plasmid

#206981

Purpose

empty pTRIPZ vector for cloning of doxycycline-inducible gene expression

Depositor

Ming Chen

Article

Loh et al Metabolites. 2023 Aug 11;13(8):939. doi:

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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BCL6-GFP in Artichoke

Plasmid

#169931

Purpose

Overexpression of Full Length of BCL6-GFP fusion

Depositor

Benjamin Ebert

Article

Slabicki et al Nature. 2020 Dec;588(7836):164-168. doi:

Insert

BCL6 (BCL6 Human)

Use

Lentiviral

Tags

GFP

Expression

Mutation

Promoter

Available since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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xCas9 3.7

Plasmid

#108379

Purpose

Mammalian expression vector for xCas9 3.7

Depositor

David Liu

Article

Hu et al Nature. 2018 Feb 28. pii: nature26155. d

Insert

xCas9 3.7

Use

Tags

NLS

Expression

Mammalian

Mutation

A262T, R324L, S409I, E480K, E543D, M694I, E1219V

Promoter

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-fDIO-GFP-IRES-tTA

Plasmid

#118026

Purpose

AAV vector for sparse and bright labeling in a cell-type specific manner

Depositor

Minmin Luo

Article

Lin et al Nat Methods. 2018 Nov 19. pii: 10.1038/s

Insert

TRE-fDIO-GFP-IRES-tTA

Use

AAV

Tags

Expression

Mutation

See depositor comments below

Promoter

TRE

Available since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-Luciferase

Plasmid

#58792

Purpose

Express FLAG-tagged Luciferase in mammalian cells

Depositor

Hiroyoshi Ariga

Article

Maita et al Anal Biochem. 2014 Feb 3. pii: S0003-269

Insert

firefly Luciferase

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 15, 2014

Availability

Academic Institutions and Nonprofits only
pIU211

Plasmid

#44036

Depositor

Dane Wittrup

Article

Boder et al Nat Biotechnol. 1997 Jun;15(6):553-7.

Insert

AGA1

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 1, 2013

Availability

Academic Institutions and Nonprofits only

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pME.E (pDHg16)

Plasmid

#24573

Depositor

David Pulleyblank

Article

Haniford et al Nature. 1983 Apr 14. 302(5909):632-4.

Insert

pME.E (pDHg16)

Use

Tags

Expression

Mutation

Promoter

Available since

May 10, 2010

Availability

Academic Institutions and Nonprofits only