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Plasmid#127354Purposegateway entry vector for making C-terminal TurboID-mVenus fusion with nuclear and non-nuclear proteinsDepositorInsertTurboID (BirA mutant)
UseGateway entry vectorTagsGS linker, V5, and mVenusExpressionMutationQ65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…Promoterno promoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSF4 TetCMV intron Renilla-STOP 24xPP7 1xfirefly-siRNA 24xMS2 SV40 CTE polyA
Plasmid#104097PurposeExpresses TREAT-PK co-localization control in mammalian cellsDepositorInsertRenilla luciferase
UseTags24xMS2 stem loops in 3'UTR and 24xPP7 stem l…ExpressionMammalianMutationPromoterAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
NowGFP /pQE-30
Plasmid#74749PurposeNowGFP gene for bacterial expressionDepositorInsertNowGFP
UseTagsHisTag (with a mutation but still working)ExpressionBacterialMutationWasCFP with substitutions E6K, L42M, T43S, V68M, …PromoterT5Available sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR_P2R-P3_R2-miniTurbo-mVenus-STOP-L3
Plasmid#127356Purposegateway entry vector for making C-terminal miniTurbo-mVenus fusion with nuclear and non-nuclear proteinsDepositorInsertminiTurbo (BirA mutant)
UseGateway entry vectorTagsGS linker, V5, and mVenusExpressionMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …Promoterno promoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
dCas9-DNMT3a-DNMT3l-3xFLAG-tagBFP
Plasmid#154139PurposeExpresses the dCas9-DNMT3a-DNMT3l-3xFLAG-tagBFP fusion protein for targeted DNA methylation.DepositorInsertdCas9, DNMT3a (catalytic domain), DNMT3l (C-terminal part), tagBFP
UseTags3x FLAG and tagBFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCR8/GW_TOPO_Cdk12_Untagged
Plasmid#127176PurposeUntagged mouse Cdk12 transgene (NM_001109626.1 isoform) cloned into Gateway entry vectorDepositorInsertMouse Cyclin Dependent Kinase 12 (Cdk12 Mouse)
UseEntry vector with transgene for gateway cloningTagsNoneExpressionMutationPromoterNoneAvailable sinceJuly 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYPQ166-SpRY
Plasmid#161520PurposeGateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zSpRYCas9-NLS without promoterDepositorInsertzSpRYCas9
UseCRISPR; Gateway compatible zsprycas9 entry cloneTags3X FLAG, NLS and NLSExpressionPlantMutationPromoterAvailable sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR_P2R-P3_R2-Turbo-NES-mVenus-STOP-L3
Plasmid#127353Purposegateway entry vector for making C-terminal TurboID-mVenus fusion with non-nuclear proteinsDepositorInsertTurboID (BirA mutant)
UseGateway entry vectorTagsGS linker, NES, V5, and mVenusExpressionMutationQ65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…Promoterno promoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
CYFIP1.mCherry
Plasmid#122051PurposeExpresses human CYFIP1 in mammalian cells.DepositorInsertCYFIP1 (CYFIP1 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
scFv-GCN4-DNMT3a-DNMT3l
Plasmid#154140PurposeExpresses the scFv-GCN4-DNMT3a-DNMT3l fusion protein (more details are shown in the vectro map) for targeted DNA methylation. Should be used in a combination with the dCas9-SunTag systems.DepositorInsertscFv-GCN4, DNMT3a (catalytic domain), DNMT3l (C-terminal part), sfGFP
UseTagsHA and sfGFPExpressionMammalianMutationPromoterSFFVAvailable sinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only