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pCC20

Plasmid

#198783

Purpose

Drives specific expression in the ASK neurons

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

Psra-9-GAL4-SK(DBD)-VP64

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pHR-hSyn-EGFP-8X2C (LV GABA mAGNET)

Plasmid

#114212

Purpose

Lentiviral vector (control) that targets expression to GABAergic inhibitory neurons in rodent cortex

Depositor

Xue Han

Article

Keaveney et al Cell Rep. 2018 Jul 10;24(2):294-303. doi

Insert

Enhanced Green Fluorescent Protein

Use

Lentiviral and Mouse Targeting

Tags

None

Expression

Mammalian

Mutation

None

Promoter

human synapsin (hSyn)

Available since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pDEST-FRT-TO-GFP-PALB2 KR

Plasmid

#71107

Purpose

mammalian expression of GFP-PALB2 mutant. (first 8 Lysine residues mutated to Arginine)

Depositor

Daniel Durocher

Article

Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

Insert

PALB2 (PALB2 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

first 8 Lysine residues mutated to Arginine (K14-…

Promoter

CMV/TO

Available since

Dec. 2, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pJet-CMV-CD5Flag2-bglpA

Plasmid

#89705

Purpose

Expression plasmid for Flag embedded into human CD52 with short polyA

Depositor

Dmitriy Mazurov

Article

Zotova et al Sci Rep. 2019 Feb 28;9(1):3132. doi: 10.

Insert

CMV-CD5Flag2-bglpA

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 1, 2019

Availability

Academic Institutions and Nonprofits only

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PGEX-6P1-hNF2-AR mutant

Plasmid

#107149

Purpose

bacterial expression

Depositor

Tina Izard

Article

Chinthalapudi et al Nat Commun. 2018 Apr 6;9(1):1338. doi: 1

Insert

neurofibromin 2 (NF2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

A585W-R588K

Promoter

Ptac

Available since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pTXB3 AurA

Plasmid

#68270

Purpose

bacterial expression of human AurA

Depositor

Stefano Ferrari

Article

Krystyniak et al Oncogene. 2006 Jan 19;25(3):338-48.

Insert

AurA (AURKA Human)

Use

Tags

Mxe intein/chitin binding domain

Expression

Bacterial

Mutation

See depositor comment below

Promoter

T7

Available since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only
pyc128m

Plasmid

#158778

Purpose

soluble GCaMP expression

Depositor

Jennifer Garrison, Zachary Knight

Article

Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

Insert

GCaMP6m

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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HSC1-GiP

Plasmid

#58254

Purpose

This self-inactivated retroviral vector has an internal human EF1alpha promoter driving the expression of a bicistronic EGFP ires Puromycin cassette

Depositor

James Ellis

Article

Rival-Gervier et al Mol Ther. 2013 Aug;21(8):1536-50. doi: 1

Insert

Enhanced Green Fluorescence Protein, Puromycin resistance gene

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Human EF1-alpha

Available since

Oct. 10, 2014

Availability

Academic Institutions and Nonprofits only

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Bongwoori

Plasmid

#63720

Purpose

Consists of the triple mutant voltage sensing domain and the optimized linker between the S4 transmembrane domain and the super ecliptic pHlorin A227D

Depositor

Bradley Baker

Article

Piao et al J Neurosci. 2015 Jan 7;35(1):372-85. doi

Insert

Bongwoori

Use

Tags

Super ecliptic pHlorin A227D

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 28, 2015

Availability

Academic Institutions and Nonprofits only
tdEos-Tubulin-C-18

Plasmid

#57683

Purpose

Localization: Microtubules, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Solowska et al J Neurosci. 2014 Jan 29;34(5):1856-67. d

Insert

Tubulin (TUBA1B Human)

Use

Tags

tdEos

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pHA-ΔN335-Kin1A

Plasmid

#31606

Depositor

Anthony Brown

Article

Uchida et al Mol Biol Cell. 2009 Dec . 20(23):4997-50

Insert

ΔN335 kinesin-1A (Kif5a Mouse)

Use

Tags

HA-tag

Expression

Mammalian

Mutation

deleted amino acids 1-335

Promoter

CMV

Available since

Oct. 4, 2011

Availability

Academic Institutions and Nonprofits only
pscALPS-SIV-RCM vpx

Plasmid

#115812

Purpose

Encodes codon optimized 3xFLAG-SIVRCM Vpx (AF349680) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVRCM Vpx (AF349680)

Use

Lentiviral

Tags

3x-FLAG

Expression

Mutation

Promoter

SFFV

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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Empty Anchor Control

Plasmid

#127665

Purpose

Encodes for puromycin resistance expressed from the EF1a promoter

Depositor

Jennifer Phillips-Cremins

Article

Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

Insert

Puro

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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pscALPS-SIV-CPZ-TAN3 vpr

Plasmid

#115833

Purpose

Encodes codon optimized 3xFLAG-SIVCPZ-TAN3 Vpr (DQ374658) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVCPZ-TAN3 Vpr (DQ374658)

Use

Lentiviral

Tags

3x-FLAG

Expression

Mutation

Promoter

SFFV

Available since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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FUP95PDZGW (C5)

Plasmid

#74021

Purpose

lentiviral expression of Psd95-EGFP fusion

Depositor

Weifeng Xu, Oliver Schluter, Robert Malenka

Article

Xu et al Neuron. 2008 Jan 24;57(2):248-62. doi: 1

Insert

Psd95 (DLG4 Rat)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

PDZ

Promoter

hUbC

Available since

April 1, 2016

Availability

Academic Institutions and Nonprofits only
pPD_Pinx-1::ChR2(H134R)::mCherry

Plasmid

#117420

Purpose

For selectively expressing ChR2(H134R) in AiB interneurons under the expression of a inx-1 promoter

Depositor

Shohei Mitani

Article

Hori et al PLoS Genet. 2018 Jun 25;14(6):e1007477.

Insert

channel rhodopsin-2 with H134R mutation

Use

Tags

Expression

Worm

Mutation

H134R

Promoter

inx-1

Available since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pCDF1-ERBB4 (E836K)

Plasmid

#29542

Depositor

Yardena Samuels

Article

Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

Insert

ERBB4 (ERBB4 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 20, 2011

Availability

Academic Institutions and Nonprofits only
pCRRNA-G20-R20

Plasmid

#101822

Purpose

Repress mCherry and GFP independently, with different strengths

Depositor

David Bikard, Sven van Teeffelen

Article

Vigouroux et al Mol Syst Biol. 2018 Mar 8;14(3):e7899.

Insert

crRNAs targeting GFP and mCherry

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

Spy 1049

Available since

Oct. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pX459_HR_Prnp_3

Plasmid

#78623

Purpose

pX459 vector encoding SpCas9-2A-Puro and a chimeric guide RNA targeting Prnp coding sequence

Depositor

Walker Jackson

Article

Kaczmarczyk et al PLoS One. 2016 Apr 29;11(4):e0154604. do

Insert

HR_Prnp_3 (Prnp Mouse)

Use

CRISPR and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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pscALPS-SIV-AGM-VER9063 vpr

Plasmid

#115825

Purpose

Encodes codon optimized 3xFLAG-SIVAGM Vpr (Ver 9063) (L40990) and Puro resistance marker

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVAGM Vpr (Ver 9063)

Use

Lentiviral

Tags

3x-FLAG

Expression

Mutation

Promoter

SFFV

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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