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Plasmid#186413PurposeMultisite destination vector for ascidian electroporation of an AttB1/B2 recombined NLSLacZ under control of an AttB3/B5 recombined reg. sequence.DepositorInsertlacZ (lacZ E. coli)
UseGateway destination vectorTagsNuclear localization signalExpressionMutationPromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
KTK_095
Plasmid#180524PurposedCas9 KTK compatible plasmid. Contains dCas9 and lacZ dropout region with flanking D1.1 overhangs for insertion of gRNA expression assemblyDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pnYC-NvM-DmBam_141-442_AG
Plasmid#148833PurposeBacterial Expression of DmBam_141-422DepositorInsertDmBam_141-422 (bam Fly)
UseTagsExpressionBacterialMutationone non silent mutation A239 to S, described as …PromoterAvailable sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCASCADE-gltA1-gltA2
Plasmid#71348PurposegltA1-gltA2 targeting guide RNA E. coli , Low Phosphate InductionDepositorInsertgltA1-gltA2 guide array
UseTagsExpressionBacterialMutationPromoterE . coli ugpB gene promoter, low phosphate induct…Available sinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 PhiC31 RL (attR:P35S:TMtb:attL) (GB1506)
Plasmid#160582Purpose35S promoter inverted sequence and the Mtb terminator flanked by two opposing PhiC31 site-specific recombination sites (attR and attL)DepositorInsertattR:P35S:TMtb:attL
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCCM’ ΔacRAF
Plasmid#162710PurposeSecond CCM operon lacking acRAF; Deletion of putative rubisco chaperone, acRAFDepositorInsertSecond CCM operon cloned from H. neapolitanus
UseTagsExpressionBacterialMutationpCCM' with a deletion of putative rubisco ch…PromoterPLtet0-1 promoterAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1820 - pDEST[2-3](5605)[Psmu-1 | smu-1 genomic | ccdB Cm]
Plasmid#159838PurposeThree-fragment Gateway compatible pDEST vector to clone transgene downstream of smu-1 for germline expression from arraysDepositorInsertpDEST[2-3](5605)[Psmu-1 | smu-1 genomic | ccdB Cm]
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDGE669
Plasmid#153235PurposeRecipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAsDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceOct. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDGE672
Plasmid#153238PurposeRecipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAsDepositorTypeEmpty backboneUseCRISPRTagsGFPExpressionPlantMutationPromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
MTK234_005
Plasmid#123992PurposeEncodes the spCas9 sgRNA1 cROSA26 (homology arm 1) gRNA expression cassette as a type 234 part to be used in the MTK systemDepositorInsertsgRNA for cROSA homology arm-1)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK234_006
Plasmid#123993PurposeEncodes the spCas9 sgRNA1 cROSA26 (homology arm 2) gRNA expression cassette as a type 234 part to be used in the MTK systemDepositorInsertsgRNA for cROSA Homology arm-2
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK234_007
Plasmid#123994PurposeEncodes the spCas9 sgRNA1 cROSA26 (homology arm 3) gRNA expression cassette as a type 234 part to be used in the MTK systemDepositorInsertsgRNA for cROSA Homology arm-3
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK234_054
Plasmid#123959PurposeEncodes the saCas9 CXCR1 (site 3) gRNA expression cassette as a type 234 part to be used in the MTK systemDepositorInsertCXCR4 sa sgRNA 3
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK234_056
Plasmid#123921PurposeEncodes the spCas9 gRNA GFP dropout expression cassette (human u6 promoter and constant region 3) as a type 234 part to be used in the MTK systemDepositorInsertgRNA-GFPDROPOUT-hu6_20N_cr3
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK234_028
Plasmid#123908PurposeEncodes the spCas9 sgRNA1 hThal5.10-2_hg18 (site 2) gRNA expression cassette as a type 234 part to be used in the MTK systemDepositorInsertsgRNA2 -hThal5.10-2_hg18
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK234_029
Plasmid#123909PurposeEncodes the spCas9 sgRNA1 hThal5.10-2_hg18 (site 3) gRNA expression cassette as a type 234 part to be used in the MTK systemDepositorInsertsgRNA3 -hThal5.10-2_hg18
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK6_013
Plasmid#123867PurposeEncodes Woodchuck Hepatitis Virus (WHP) Posttranscriptional Regulatory Element (WPRE) expressing a Blasticidin resistance cassette without a terminator to be used in the MTK systemDepositorInsertWPRE-Blast (no terminator)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK3_018
Plasmid#123733PurposeEncodes rapamycin and tamoxifen activated split-dCAS9 (N-terminal) as a Type 3 part to be used in the MTK systemDepositorInsertspdCas9(1-204)-frb-D10A-ERT2
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK3_019
Plasmid#123734PurposeEncodes tamoxifen activated split-dCAS9 (N-Terminal) as a Type 3 part to be used in the MTK systemDepositorInsertspdCas9(1-204)-ERT2
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK1_015
Plasmid#123678PurposeEncodes ConLS, chicken hypersensitivity site 4 insulator and locus of X-over P1 (loxP) as a Type 1 part to be used in the MTK systemDepositorInsertConnector LS-2xHS4-LoxP
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only