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Plasmid#211494PurposeExpression of EGFP-tagged GRAM domain of GRAMD1b (EGFP-GRAM1b) in mammalian cellsDepositorInsertGRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
SpyTag003(M115G)-Talin rod-mCherry
Plasmid#210026PurposeExpression of SpyTag003(M115G)-Talin rod(434-2541)-mCherry in mammalian cellsDepositorInsertSpyTag003(M115G)-Talin1 rod domain(434-2541)-mCherry
UseTagsExpressionMammalianMutationSpyTag003 M115G mutation to modify SpyCatcher003 …PromoterCMVAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
SpyTag003(M115A)-Talin rod-mCherry
Plasmid#210025PurposeExpression of SpyTag003(M115A)-Talin rod(434-2541)-mCherry in mammalian cellsDepositorInsertSpyTag003(M115A)-Talin1 rod domain(434-2541)-mCherry
UseTagsExpressionMammalianMutationSpyTag003 M115A mutation to modify SpyCatcher003 …PromoterAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-SUMO-B'-l2-B'
Plasmid#209119PurposeBacterial expression of CC-GEMS ligand SUMO-B'-l2-B'DepositorInsertSUMO-B'-l2-B'
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLeo-A-GEMS(JAK-STAT)
Plasmid#209109PurposeTransient mammalian expression of the CC functionalized GEMS receptor A-GEMS (JAK-STAT)DepositorInsertA-GEMS(JAK-STAT)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
LLP786_SpyCatcher-EZH2-FL-CO
Plasmid#211779PurposeSpyTag counterpart binding domain, SpyCatcher, fused to polycomb repressive complex subunit EZH2, with GFP selectionDepositorInsertaGCN4-KRAB
UseTags3xTy1ExpressionMammalianMutationLast 300 bp codon-optimised (CO) to detect KRAB e…PromoterpEF1a and pSV40Available sinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
LLP787_SnoopCatcher-EZH2-FL-CO
Plasmid#211780PurposeSnoopTag counterpart binding domain, SnoopCatcher, fused to polycomb repressive complex subunit EZH2, with GFP selectionDepositorInsertaGCN4-DNMT3A
UseTags3xTy1ExpressionMammalianMutationLast 300 bp codon-optimised (CO) to detect DNMT3A…PromoterpEF1a and pSV40Available sinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
WT_IFL_A28C_R
Plasmid#200184PurposeWT PA14 I-F Leader and A28C RepeatDepositorInsertWildtype I-F Leader and a mutant I-F Locus (A28C)
UseTagsExpressionBacterialMutationA28C in the I-F repeatPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
PB-CAG-4300-L1-G1397N
Plasmid#210396PurposeTo correct m.A4300G mutation on mtDNADepositorInsert4300-L1-G1397N
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
PB-CAG-4300-H2-G1397C
Plasmid#210395PurposeTo correct m.A4300G mutation on mtDNADepositorInsert4300-H2-G1397C
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-SUMO-G'-l2-G'
Plasmid#209120PurposeBacterial expression of CC-GEMS ligand SUMO-G'-l2-G'DepositorInsertSUMO-G'-l2-G'
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLeo-A-a3-GEMS(JAK-STAT)
Plasmid#209111PurposeTransient mammalian expression of the CC functionalized GEMS receptor A-a3-GEMS (JAK-STAT)DepositorInsertA-a3-GEMS(JAK-STAT)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1 IntS12
Plasmid#210521PurposeExpresses GST-tagged Drosophila IntS12DepositorInsertIntS12 (IntS12 Fly)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceDec. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP Cav2.2 DomI + DomII pMT2
Plasmid#206095Purposeexpression of the N-terminus, Domain I, Domain II and the II-III loop (amino acids 1-1154) of rabbit Cav2.2 calcium channel with a N-terminal GFP tagDepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsGFPExpressionMammalianMutationPromoterAd MLP/TPL/SV40Available sinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR-SUMO-A'-l2-A'-IRES-EmGFP
Plasmid#209125PurposeLentiviral vector including gene for CC-GEMS ligand SUMO-A'-l2-A' and EmGFPDepositorInsertSUMO-A'-l2-A'-IRES-EmGFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
phROSA26-Tet-HA-JLP_WT
Plasmid#208770PurposeTetracycline inducible expression vector for HA-tagged wild-type JLP protein (HA-JLP_WT), used as a donor plasmid for HA-JLP_WT knock-in at the human ROSA26 locusDepositorInsertJLP (SPAG9 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pet28b_His_RAD23A-UBA1
Plasmid#201549PurposeExpresses UBA1 of human RAD23A in E. coliDepositorInsertUV excision repair protein RAD23 homolog A (RAD23A Human)
UseTags6x HisExpressionBacterialMutationExpresses amino acids 160-205 of DNA polymerase i…PromoterT7Available sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pet28b-His_RAD23A-UBA2
Plasmid#201550PurposeExpresses UBA2 of human RAD23A in E. coliDepositorInsertUV excision repair protein RAD23 homolog A (RAD23A Human)
UseTags6x HisExpressionBacterialMutationExpresses amino acids 311-363 of DNA polymerase i…PromoterT7Available sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-DDK_S172A_M173A RAD23A
Plasmid#201574PurposeExpresses a variant of human RAD23A containing mutations S172A and M173A within UBA1. Variant of plasmid pCMV6-AN-DDK_WT RAD23ADepositorInsertUV excision repair protein RAD23 homolog A (RAD23A Human)
UseTagsFLAGExpressionMammalianMutationContains mutations S172A and M173APromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-DDK_V195A RAD23A
Plasmid#201575PurposeExpresses a variant of human RAD23A containing mutation V195A within UBA1. Variant of plasmid pCMV6-AN-DDK_WT RAD23ADepositorInsertUV excision repair protein RAD23 homolog A (RAD23A Human)
UseTagsFLAGExpressionMammalianMutationContains mutation V195APromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only