-
Plasmid#218656PurposeKnockout vector for human PLEKHA3 (FAPP1)DepositorInsertPLEKHA3 (PLEKHA3 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV UBC OSBP(PH)-APEX2-3xFLAG-IRES-eGFP
Plasmid#218637PurposeProximity biotinylation control of OSBP(PH domain)DepositorInsertOSBP (OSBP Human)
UseLentiviralTagsAPEX2-3xFLAGExpressionMammalianMutationPH domain onlyPromoterUBCAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNIC-OmpA-NHSTIIT-GG
Plasmid#215790PurposeCloning of target gene with N terminal OmpA SEC pathway signal peptide His6 Twin Strep tag and TEV cleavageDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pNIC-SufI-NHSTIIT-GG
Plasmid#215791PurposeCloning of target gene with N terminal SufI TatA pathway signal peptide His6 Twin Strep tag and TEV cleavageDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pNIC- AVITAG-CTH10STII- -GG
Plasmid#215805PurposeCloning of target gene with C terminal non cleavable AVITAG and TEV cleavage of C terminal His10 and Twin Strep tagDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pNIC-HIS6-SUMO1-AVITAG-GG
Plasmid#215808PurposeCloning of target gene with N terminal His6 SUMO2 tag and SENP1 cleavage non cleavable N-terminal avitagDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTwist-msEEA1
Plasmid#214991PurposeGateway entry vector with mouse EEA1DepositorInsertEEA1 (Eea1 Mouse)
UseGatewayTags3xFLAGExpressionMutationPromoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIA035
Plasmid#200426PurposeamyE::(Ppen-lacIΔ11-dronPA cm). PALM integration vector includes dronPA fusion tagged C-terminal of LacI for FROS imaging in B. subtilis.DepositorArticleInsertLacI
UseTagsdronPAExpressionBacterialMutationPromoterPpenAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIA030
Plasmid#200424PurposethrC::(Ppen-lacIΔ11-PAmCherry1 erm2). PALM integration vector includes PAmCherry1 fusion tagged C-terminal of LacI for FROS imaging in B. subtilis.DepositorArticleInsertLacI
UseTagsPAmCherryExpressionBacterialMutationPromoterPpenAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
nTALE+CD_for TUB_8N_1397NC
Plasmid#193383PurposenTALECD (1397NC) expression vectorDepositorInsertFor multiplex C-to-T base editing to introduce amino acid mutations in the coding sequence of AtTUBs.
UseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDESTpK7WG2-RPS5Ap-NLS-OleGFP
Plasmid#193370PurposenTALEN or nTALECD vector assembly, Destination vector for plants to express ORFs from entry clone with NLS under control of the RPS5A promoter and HSP terminator.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
E2_pENTR_R4-R3_HSPter-RPS5A-NLS
Plasmid#193371PurposenTALEN or nTALECD vector assembly, Entry clone for plants to express ORFs from entry clone with NLS under control of the RPS5A promoter and HSP terminator.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
nTALECD for_CYO1 exon1_1397NC
Plasmid#193376PurposenTALECD (1397NC) expression vectorDepositorInsertFor specific C-to-T base editing to introduce a nonsense mutation in the coding sequence of AtCYO1.
UseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
nTALECD for_CYO1 exon2_1397NC
Plasmid#193377PurposenTALECD (1397NC) expression vectorDepositorInsertFor specific C-to-T base editing to introduce a nonsense mutation in the coding sequence of AtCYO1.
UseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
nTALECD for_PKT3 exon1_1397NC
Plasmid#193378PurposenTALECD (1397NC) expression vectorDepositorInsertFor specific C-to-T base editing to introduce a nonsense mutation in the coding sequence of AtPKT3.
UseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
nTALECD for_PKT3 exon12_1397NC
Plasmid#193379PurposenTALECD (1397NC) expression vectorDepositorInsertFor specific C-to-T base editing to introduce an amino acid mutation in the coding sequence of AtPKT3.
UseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
nTALECD for_MSH1 exon5_1397NC
Plasmid#193380PurposenTALECD (1397NC) expression vectorDepositorInsertFor specific C-to-T base editing to introduce an amino acid mutation in the coding sequence of AtMSH1.
UseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
nTALECD for_MSH1 exon21_1397NC
Plasmid#193381PurposenTALECD (1397NC) expression vectorDepositorInsertFor specific C-to-T base editing to introduce an amino acid mutation in the coding sequence of AtMSH1.
UseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBTK1051
Plasmid#209541PurposeBTK Type 5 homology arm for Golden Gate assemblyDepositorInsertRecombination into the S. alvi wkB2 genome within the SALWKB2_RS11215/SALWKB2_RS11220 intergenic region
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 2, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBTK1059
Plasmid#209549PurposeBTK Type 5 homology arm for Golden Gate assemblyDepositorInsertDownstream homology arm for recombination into the Snodgrassella alvi wkB2 genome to replace clpA (SALWKB2_RS04465)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 1, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits