Addgene

We narrowed to 933 results for: TagBFP

Showing: 641 - 660 of 933 results

Results per page:

pKLV2-U6gRNA5(Tsc2-g2)-PGKpuroBFP-W

Plasmid

#105040

Purpose

Lentiviral gRNA plasmid targeting mouse Tsc2 , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Tsc2 (Tsc2 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Availability

Academic Institutions and Nonprofits only

Enlarge
pKLV2-U6gRNA5(Rictor-g1)-PGKpuroBFP-W

Plasmid

#105041

Purpose

Lentiviral gRNA plasmid targeting mouse Rictor , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Rictor (Rictor Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Availability

Academic Institutions and Nonprofits only

Enlarge
pKLV2-U6gRNA5(Rictor-g2)-PGKpuroBFP-W

Plasmid

#105042

Purpose

Lentiviral gRNA plasmid targeting mouse Rictor , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Rictor (Rictor Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Availability

Academic Institutions and Nonprofits only

Enlarge
pKLV2-U6gRNA5(Stk11)-PGKpuroBFP-W

Plasmid

#105043

Purpose

Lentiviral gRNA plasmid targeting mouse Stk11 , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Stk11 (Stk11 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR-U6gRNA-PGKpuro2ABFP

Plasmid

#136405

Purpose

Donor vector for gRNA subcloning. Required for gRNA cloning into the RCAS-DV. Also constitutively expresses Puromycin linked to TagBFP.

Depositor

Massimo Squatrito

Article

Oldrini et al Nat Commun. 2018 Apr 13;9(1):1466. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

BbsI restriction site at position 437 was removed…

Promoter

U6, PDK

Availability

Academic Institutions and Nonprofits only

Enlarge
JPF0466

Plasmid

#124037

Purpose

Encodes pEF1a driving expression of nuclear iRFP713, and mu6 expression of a tagBFP-targeting sgRNA in a 2nd gen. Lenti Virus destination vector

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pHR_pEF1a-iRFP713_tagBFP sgRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
JPF0468

Plasmid

#124038

Purpose

Encodes pEF1a driving expression of nuclear iRFP713, and mu6 expression of tagBFP- and mScarlet-targeting sgRNAs in a 2nd gen. Lenti Virus destination vector

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pHR_pEF1a-iRFP713_mScarletsgRNA_tagBFP sgRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG_NLS-Cas9-XTEN-Ec73RT-NLS_pA_pCMV_BFP_pA

Plasmid

#176458

Purpose

Vector for encoding a human codon-optimized SpCas9-XTEN-Ec73RT fusion driven by CAG promoter and tagBFP driven by CMV promoter

Depositor

Hui Yang

Article

Kong et al Protein Cell. 2021 Aug 17. pii: 10.1007/

Insert

humanized S. pyogenes Cas9-XTEN-Ec73RT fusion

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Availability

Academic Institutions and Nonprofits only

Enlarge
pMax_BFP_A191G

Plasmid

#177823

Purpose

Site-specific mutagenesis of tagBFP for the purposes of creating a site-specific U:A mispair for the purposes of measuring UDG activity via Host Cell Reactivation (HCR).

Depositor

Leona Samson, Zachary Nagel

Article

Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

Insert

tagBFP_A191G

Use

Tags

Expression

Mammalian

Mutation

A191G

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pCA12-pHR-SFFV-BFP-B23

Plasmid

#199443

Purpose

Expression of BFP-B23 in mammalian cells

Depositor

Baohui Chen

Article

Fu et al J Cell Biol. 2023 Jan 2;222(1):e20220211

Insert

B23 (NPM1 Human)

Use

Lentiviral

Tags

TagBFP

Expression

Mammalian

Mutation

Promoter

SFFV

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR-SFFV-dCas9-BFP

Plasmid

#46910

Purpose

Human expression vector containing SFFV promoter, dCas9 that is fused to 2x NLS and tagBFP

Depositor

Stanley Qi, Jonathan Weissman

Article

Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

Insert

dCas9-BFP fusion

Use

CRISPR and Lentiviral

Tags

2xNLS, BFP, and HA

Expression

Mammalian

Mutation

Promoter

SFFV

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR-SFFV-dCas9-BFP-KRAB

Plasmid

#46911

Purpose

Human expression vector containing SFFV promoter, dCas9 that is fused to 2x NLS, tagBFP and a KRAB domain

Depositor

Stanley Qi, Jonathan Weissman

Article

Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

Insert

dCas9-BFP-KRAB fusion

Use

CRISPR and Lentiviral

Tags

2xNLS, BFP, HA, and KRAB domain

Expression

Mammalian

Mutation

Promoter

SFFV

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONOR-SNCAe2-WT

Plasmid

#85845

Purpose

Donor plasmid for SNCA exon2 wild type sequence. Also contains TagBFP and EGFP

Depositor

Jens Schwamborn

Article

Arias-Fuenzalida et al Stem Cell Reports. 2017 Nov 14;9(5):1423

Insert

SNCA exon 2 homology arms (SNCA Human)

Use

Tags

-

Expression

Bacterial and Mammalian

Mutation

-

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONOR-SNCAe3-WT

Plasmid

#85847

Purpose

Donor plasmid for SNCA exon3 wild type sequence. lso contains TagBFP and dTomato

Depositor

Jens Schwamborn

Article

Arias-Fuenzalida et al Stem Cell Reports. 2017 Nov 14;9(5):1423

Insert

SNCA exon 3 homology arms (SNCA Human)

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONOR-SNCAe3-A53T

Plasmid

#85848

Purpose

Donor plasmid for SNCA exon3 A53T sequence. Also contains EGFP and tagBFP

Depositor

Jens Schwamborn

Article

Arias-Fuenzalida et al Stem Cell Reports. 2017 Nov 14;9(5):1423

Insert

SNCA exon 3 homology arms (SNCA Human)

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
Fuw-dCas9-Tet1CD-P2A-BFP

Plasmid

#108245

Purpose

Lentiviral vector to express dCas9-Tet1CD-P2A-tagBFP

Depositor

Rudolf Jaenisch

Article

Liu et al Cell. 2018 Feb 8. pii: S0092-8674(18)300

Insert

dCas9-Tet1CD-HA-P2A-BFP

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

dCas9-D10A, dCas9-H840A

Promoter

Ubc

Availability

Academic Institutions and Nonprofits only

Enlarge
pKLV2-U6gRNA5(Kcmf1-g1)-PGKpuroBFP-W

Plasmid

#105017

Purpose

Lentiviral gRNA plasmid targeting mouse Kcmf1 , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Kcmf1 (Kcmf1 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Availability

Academic Institutions and Nonprofits only

Enlarge
pKLV2-U6gRNA5(Apc-g1)-PGKpuroBFP-W

Plasmid

#105022

Purpose

Lentiviral gRNA plasmid targeting mouse Apc , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Apc (Apc Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Availability

Academic Institutions and Nonprofits only

Enlarge
pKLV2-U6gRNA5(Pou5f1-g1)-PGKpuroBFP-W

Plasmid

#105035

Purpose

Lentiviral gRNA plasmid targeting mouse Pou5f1 , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Pou5f1 (Pou5f1 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Availability

Academic Institutions and Nonprofits only

Enlarge
pKLV2-U6gRNA5(Pou5f1-g2)-PGKpuroBFP-W

Plasmid

#105036

Purpose

Lentiviral gRNA plasmid targeting mouse Pou5f1 , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Pou5f1 (Pou5f1 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Availability

Academic Institutions and Nonprofits only

Enlarge