These results may also be relevant.
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pBa.Halo-Kif13B tail 442-1843
Plasmid#162721PurposeMammalian expression of WT mouse Kif13b with deletion of the 441 N-terminal residuesDepositorInsertKif 13B (Kif13b Mouse)
UseTagsExpressionMammalianMutationWT with deletion of the 441 N-terminal residues.PromoterChicken Beta actinAvailable sinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pU6-1 (GB1204)
Plasmid#75405PurposeProvides the A. thaliana U6-1 RNA polIII promoter as a level 0 GoldenBraid partDepositorInsertAtU6-1 promoter
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
Hoxa4-3'UTR
Plasmid#31524DepositorInsertHoxa4-3'UTR (Hoxa4 Mouse)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterAvailable sinceOct. 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
OptoProfilin.S138E
Plasmid#208287PurposeExpresses Cry2(1-531)-mCherry-Profilin.S138EDepositorUseTagsmCherryExpressionMammalianMutationSer 138 (sometimes numbered 137) mutated to GluPromoterCMVAvailable sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
OptoProfilin.S138A
Plasmid#208288PurposeExpresses Cry2(1-531)-mCherry-Profilin.S138ADepositorUseTagsmCherryExpressionMammalianMutationSer 138 (sometimes numbered 137) mutated to AlaPromoterCMVAvailable sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
5'-3xFLAG Tcf12+exon minimal CMV pCDNA5
Plasmid#212077PurposeLR vector for integration of Tcf12+exon(72nt) into N2a FRT rtTA3 expression cellsDepositorInsertTcf12+exon(72nt) (Tcf12 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
5'-3xFLAG Tcf12-exon minimal CMV pCDNA5
Plasmid#212076PurposeLR vector for integration of Tcf12-exon into N2a FRT rtTA3 expression cellsDepositorInsertTcf12-exon (Tcf12 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
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KIF13B 1-584
Plasmid#163908PurposeMotile fragment of mouse KIF13B (aa 1-584) for purification from insect cells.DepositorInsertkif13b (Kif13b Mouse)
UseTagsCleavable 6 x HIS tagExpressionInsectMutationPromoterpolyhedrinAvailable sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
EC71102
Plasmid#154065PurposeLevel 0 Golden Gate vector; Unmodifed CREDepositorInsertCRE
UseSynthetic Biology; Standard cloning vector used b…TagsExpressionMutationAll BsaI, Esp3I and BPiI restriction sites were r…PromoterAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
AdMKP-3 S159A
Plasmid#131520PurposeExpresses MKP-3 with S159A mutation in mammalian cellsDepositorInsertMitogen-activated protein kinase phosphatase 3 (Dusp6 Mouse)
UseAdenoviralTagsNoExpressionMutationchanged Serine 159 to AlaninePromoterCMVAvailable sinceOct. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGB3omega1R Tnos:NptII:Pnos-SF (GB1181)
Plasmid#75471PurposeTranscriptional unit for kanamycin resistance geneDepositorInsertNptII
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAgrobacterium tumefaciens Nopaline synthase promo…Available sinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMAL-PIAL2M-wt
Plasmid#67909PurposeInducible expression of PIAL2 in E.coliDepositorInsertPIAL2 (AT5G41580 Mustard Weed)
UseTagsMBPExpressionBacterialMutationE281-A496PromoterP-lacAvailable sinceAug. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGES401
Plasmid#190198PurposeFor CRISPR-Cas9 mediated multiple gene editing in soybean, single transcript unit system driven by soybean elongation factor 1A promoter (pM4), Basta selectionDepositorInsertspCas9
UseCRISPRTags3xFlagExpressionPlantMutationplant-codon optimizedPromoterGlycine max elongation factor 1A(pM4)Available sinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Vamp3-pHluorin
Plasmid#190152PurposeExpresses (pH-sensitive) super ecliptic pHluorin-tagged rat Vamp3 for imaging exocytosisDepositorInsertVamp3 (Vamp3 Rat)
UseAAVTagssuperecliptic pHluorinExpressionMammalianMutationPromoterCMVAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
FH-Tet2-pEF
Plasmid#41710PurposeThis plasmid expresses mouse Tet2 with N-terminal FLAG and HA tags in a mammalian systemDepositorInserttet methylcytosine dioxygenase 2 (Tet2 Mouse)
UseTagsFLAG and HAExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
CRY2clust-mCherry-RANK
Plasmid#208612PurposeMammalian expression of CRY2clust-mCherry-RANK (Opto-RANKc)DepositorInsertCRY2clust-mCherry-RANK (Cry2 Mouse, Mustard Weed)
UseTagsmCherryExpressionMammalianMutationPromoterCAGAvailable sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-mTET2 (CD)
Plasmid#89736PurposeExpresses the CD-domain truncation to mouse TET2 in mammalian cellsDepositorInsertTET2 (Tet2 Mouse)
UseTagsFlagExpressionMammalianMutationCD (cysteine-rich, dioxygenase domain) truncation…PromoterAvailable sinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
Raichu-OsRac1 CA
Plasmid#133264PurposeConstitutively-active (CA) mutant of OsRac1; Acts as a positive control for small GTPases activation assays.DepositorInsertOsRac1(G19V) (LOC4325879 Oryza sativa)
UseTagsCFP-lipid and Venus-CRIBExpressionPlantMutationOsRac1 G19VPromoterUbiAvailable sinceJan. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
Raichu-OsRac1 DN
Plasmid#133263PurposeDominant-negative (DN) mutant of OsRac1; Acts as a negative control for small GTPases activation assaysDepositorInsertOsRac1(T24N) (LOC4325879 Oryza sativa)
UseTagsCFP-lipid and Venus-CRIBExpressionPlantMutationOsRac1 T24NPromoterUbiAvailable sinceJan. 24, 2020AvailabilityAcademic Institutions and Nonprofits only
