Showing: 641 - 660 of 18051 results
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Plasmid#52224PurposeExpression of sgRNA precursor with CspCI placeholder for target cloning (see comments).DepositorInsertrrk1-sgRNA
UseCRISPRTagsExpressionYeastMutationPromoterrrk1AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-GCaMP6s-CAAX
Plasmid#52228PurposePlasma membrane targeted GCaMP6s, calcium sensorDepositorInsertGCaMP6s-CAAX
UseTags6xHis and CAAXExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
HBT-pcoCas9
Plasmid#52254Purposetransient expression of pcoCas9 gene in plant cellsDepositorInsertpcoCas9
UseCRISPR; Plant expressionTagsFLAG and NLSExpressionMutationPromoterhybrid constitutive promoter 35SPPDKAvailabilityAcademic Institutions and Nonprofits only -
pUC119-gRNA
Plasmid#52255PurposeCan use a PCR template to assemble new desired guide RNA. Contains an Arabidopsis U6 promoter to drive guide RNA (targeting AtPDS3 gene target site 1) expression with a TTTTTT as terminator.DepositorInsertguide RNA targeting AtPDS3 (PDS3 Mustard Weed)
UseCRISPR; Plant expressionTagsExpressionMutationPromoterArabidopsis U6 polymerase III promoterAvailabilityAcademic Institutions and Nonprofits only -
pFGC-pcoCas9
Plasmid#52256PurposeA binary plasmid containing 35SPPDK:pcoCas9:NOS cassette for plant expression of Cas9 and multiple cloning sites for insertion of guide RNADepositorInsert35SPPDK:pcoCas9:NOS cassette
UseCRISPR; Plant expressionTagsExpressionMutationPromoterconstitutive 35SPPDK promoterAvailabilityAcademic Institutions and Nonprofits only -
pSUMO1
Plasmid#52258PurposeBacterial expression of human 6His-tagged SUMO1, SUMO-E1 and SUMO-E2DepositorUseTags6His-tagExpressionBacterialMutationC-terminal gly-glyPromoterT7 and rbsAvailabilityAcademic Institutions and Nonprofits only -
pSUMO2
Plasmid#52259PurposeBacterial expression of human 6His-tagged SUMO2, SUMO-E1 and SUMO-E2DepositorUseTags6His-tagExpressionBacterialMutationC-terminal gly-glyPromoterT7 and rbsAvailabilityAcademic Institutions and Nonprofits only -
pSUMO3
Plasmid#52260PurposeBacterial expression of human 6His-tagged SUMO3, SUMO-E1 and SUMO-E2DepositorUseTags6His-tagExpressionBacterialMutationC-terminal gly-glyPromoterT7 and rbsAvailabilityAcademic Institutions and Nonprofits only -
pSA1
Plasmid#52261PurposeBacterial expression of human 6His-tagged SUMO1 and SUMO-E1DepositorUseTags6His-tagExpressionBacterialMutationC-terminal gly-glyPromoterT7 and rbsAvailabilityAcademic Institutions and Nonprofits only -
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pGEX-hXRCC1
Plasmid#52274PurposeBacterial expression of human XRCC1 with N-terminal GST-tagDepositorInsertXRCC1 (XRCC1 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailabilityAcademic Institutions and Nonprofits only -
Split 'n coexpress CUL3-RBX1
Plasmid#52293Purposebacterial co-expression of N-terminal Cul3, C-terminal Cul3, and Rbx1DepositorUseTagsGST, IRES, and TEVExpressionBacterialMutationcontains aa 1-309, contains aa 310-768, and conta…PromoterIRES and tacAvailabilityAcademic Institutions and Nonprofits only -
pCAG-hGPR56-IRES-GFP
Plasmid#52297PurposeExpresses human GPR56 and GFP in mammalian cells.DepositorInsertHuman GPR56 coding sequence (ADGRG1 Human)
UseTagsExpressionMammalianMutationPromoterCAG promoterAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-flag-YTHDF2
Plasmid#52300Purposefull length YTHDF2 with N-terminal flag tag for mammalian cell expressionDepositorInsertYTHDF2 (YTHDF2 Human)
UseTagsflagExpressionMammalianMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pGEx-4T-1-YTHDF2
Plasmid#52301PurposeEncode YTHDF2 isoform 2 with N-terminal GST tag for E. coli expressionDepositorInsertYTHDF2 (YTHDF2 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-flag-YTHDF2-C
Plasmid#52303PurposeFlag-tagged YTHDF2 C-terminal domain, from E384 to the endDepositorInsertYTHDF2 (YTHDF2 Human)
UseTagsflagExpressionMammalianMutationDeleted amino acids 1-383PromoterT7AvailabilityAcademic Institutions and Nonprofits only -
EGR-1 TurboRFP
Plasmid#52306PurposeFluid Shear Stress inducible Red Fluorescent Protein for mammalian expressionDepositorInsertEGR-1 Promoter (Egr1 Mouse)
UseTagsTurboRFPExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pET-MBP-mSA2
Plasmid#52319PurposeSolubly expresses monomeric streptavidin containing S25H mutation in E. coli as an MBP fusion. App Microbiol & Biotech 98, 6285-6295 (2014)DepositorInsertmonomeric streptavidin 2
UseTagsFLAG, His, MBP, and TEVExpressionBacterialMutationS25H mutation compared to streptavidinPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pET-Trx-mSA2
Plasmid#52320PurposeSolubly expresses monomeric streptavidin containing S25H mutation in E. coli as a thioredoxin fusion. App Microbiol & Biotech 98, 6285-6295 (2014)DepositorInsertmonomeric streptavidin 2
UseTagsFLAG, His, S tag, TEV site, and ThioredoxinExpressionBacterialMutationS25H mutation compared to streptavidinPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pTT5-hSDC1
Plasmid#52326Purposeexpresses human syndecan 1 in HEK293-EBNA1 (293E) suspension culture.DepositorInsertSDC1 (SDC1 Human)
UseTagsExpressionMammalianMutationR117Q mutation (R95Q according to numbering in Fi…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only
Showing: 641 - 660 of 18051 results