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pBS-hsFlt3L
Plasmid#25074DepositorInserthuman soluble FMS-like tyrosine kinase 3 ligand (FLT3LG Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT/TO Plk4 ∆24-mCherry
Plasmid#80271Purposemammalian expression plasmid for Plk4 with multi-phospho degron deletedDepositorInsertPlk4 (PLK4 Human)
UseTagsmCherryExpressionMammalianMutationdeletion of multi-phospho degron (amino acids 282…PromoterCMVAvailable sinceAug. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
PB-CAG::Nucbow
Plasmid#158996PurposeMulticolor nuclear-restricted labeling with a genome-integrating piggyBac transposon vector (randomly expressing H2B-tethered mEYFP, mCherry or mCerulean upon Cre recombination)DepositorInsertNucbow
UseTagsAll FPs are fused to H2BExpressionMammalianMutationPromoterCAGAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
TRE-DIO-eYFP
Plasmid#117383PurposeAn AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein eYFPDepositorHas ServiceAAV1InsertEYFP
UseAAVTagsExpressionMammalianMutationPromoterTREAvailable sinceOct. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAM-AAV-hSyn1-EGFP
Plasmid#153205PurposeHuman synapsin-1 (hSyn1) promoter-mediates gene expression in retinal neurons with fluorescent reporterDepositorTypeEmpty backboneUseAAVTagsExpressionMammalianMutationPromoterAvailable sinceJuly 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET32a-TRXtag
Plasmid#11516DepositorInsertNone - thioredoxin is vector encoded (trxA )
UseTagsHis, His (out of frame), and TrxExpressionBacterialMutationvector modified; removed N-terminal His tag by di…PromoterAvailable sinceApril 28, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 MEKK1 KD
Plasmid#12180DepositorInsertMEKK1 (Map3k1 Mouse)
UseTagsHis and XpressExpressionMammalianMutationKinase dead: K1253M; V1467LPromoterAvailable sinceSept. 22, 2006AvailabilityAcademic Institutions and Nonprofits only -
p2xFLAGhYAP1-WW mutant
Plasmid#17792DepositorInsertYes-kinase associated protein (YAP1 Human)
UseTags2xFlagExpressionMammalianMutationTryptophan 199 to Alanine; Proline 202 to AlaninePromoterAvailable sinceMay 9, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGI3EM09
Plasmid#135482PurposeAgrobacterium T-DNA with SpunHsp70pr driving hph-GFPDepositorInsertSpunHsp70pr hph-GFP ADH1t
UseTagsGFPExpressionBacterial and YeastMutationPromoterSpunHsp70prAvailable sinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-mTurquoise2
Plasmid#99113PurposeAn AAV genome with tet-inducible expression of the fluorescent protein mTurquoise2DepositorInsertmTurquoise2
UseAAVTagsExpressionMammalianMutationPromoterTREAvailable sinceJuly 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
FLAG-OTUB1 C91S-pcDNA3.1
Plasmid#118210PurposeExpresses FLAG-OTUB1 C91S catalytic inactive mutant in mammalian cellsDepositorInsertOTUB1 (OTUB1 Human)
UseTags3x FLAGExpressionMammalianMutationchanged cysteine 91 to serinePromoterAvailable sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-rTH-tTA
Plasmid#133268PurposeAn AAV genome that expresses tTA from the rat TH promoterDepositorInserttTA
UseAAVTagsExpressionMammalianMutation*(see below)PromoterrTHAvailable sinceNov. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGI3EM18
Plasmid#135484PurposeAgrobacterium T-DNA with SpunH2Bpr driving hph-tdTomatoDepositorInsertSpunH2Bpr hph-tdTomato ADH1t
UseTagstdTomatoExpressionBacterial and YeastMutationPromoterSpunH2BprAvailable sinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMT006
Plasmid#64761PurposeRecombination-Induced Tag Exchange (RITE) Cassette, 2xFLAG to an HA-6xHIS epitope tag, Hygro selectionDepositorInsertRITE cassette
UseRite cassetteTagsExpressionMutationPromoternoneAvailable sinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLHCX-Flag-mPKM2
Plasmid#42512DepositorInsertPyruvate Kinase M2
UseRetroviralTagsFlagExpressionMutationPromoterCMVAvailable sinceMarch 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLX302 GDF11-V5 puro
Plasmid#83097PurposeLentiviral vector for constitutive expression of human GDF11 with C-terminal V5 tagDepositorInsertGDF11 (GDF11 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAF0084 Ef1a-Cp36-2a-EGFP
Plasmid#193462PurposeExpression of RecombinaseDepositorInsertCp36
UseTagsExpressionMammalianMutationPromoterEf1aAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
GFP-PKI WT
Plasmid#118300PurposeExpression of PKIaDepositorInsertPKI wt
UseTagsFlag and GFPExpressionMammalianMutationPromoterAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 CARD8
Plasmid#169962PurposepDONR221 for Gateway shuttling. Contains the CARD8 T60 isoform (no stop codon)DepositorInsertCARD8 (CARD8 Human)
UseGateway cloning vectorTagsExpressionMutationPromoterAvailable sinceJuly 28, 2021AvailabilityAcademic Institutions and Nonprofits only
