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Showing: 6561 - 6580 of 6923 results

  1. pDEST17-His10-Smt3-ccdB-SNAP

    Plasmid
    #166148
    Purpose
    Gateway destination vector for generation of N-terminal His10-Smt3 and C-terminal SNAP tagged proteins in E. coli.
    Depositor
    Daniel Jarosz
    Article
    Itamar Harel et al BioRxiv
    Type
    Empty backbone
    Use
    Tags
    His10-Smt3 and SNAP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    166148-plasmid-map-sequence-id-328896

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  9. pBBR1.cas9-NT

    Plasmid
    #197871
    Purpose
    Constitutive expression of Cas9 endonuclease, trancRNA and crRNA with a randomly generated spacer sequence (non-targeting)
    Depositor
    Baojun Wang
    Article
    Huan et al ACS Synth Biol. 2023 Mar 17;12(3):709-72
    Inserts
    trancCRNA
    Cas9
    crRNA (non-chromosomal (E. coli/S. flexneri)-targeting spacer sequence)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    197871-plasmid-map-sequence-id-396456

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  18. pPlacPbuCas13b-gRNA-NT

    Plasmid
    #184838
    Purpose
    Expression of single-spacer CRISPR array with a shortened non-targeting spacer for PbuCas13b and expression of PbuCas13b in bacteria.
    Depositor
    Chase Beisel
    Article
    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2
    Insert
    Co-expression of single-spacer CRISPR array with a nontargeting spacer for PbuCas13b and PbuCas13b nuclease in bacteria.
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119 and lac promoter
    Available since
    Oct. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    184838-plasmid-map-sequence-id-373981

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  19. pSBK.136

    Plasmid
    #187220
    Purpose
    Expresses Eco1 ncRNA v35 (long a1/a2) and Eco1 ncRNA v35 (long a1/a2, barcode 6) from promoters pSalTTC and pTet*, respectively
    Depositor
    Seth Shipman
    Article
    Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:
    Insert
    Retron Eco1 ncRNA v35 (long a1/a2); Retron Eco1 ncRNA v35 (long a1/a2, barcode 6)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    A: pSalTTC; B: pTet*
    Available since
    Sept. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    187220-plasmid-map-sequence-id-368082

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  20. KTK_139

    Plasmid
    #180565
    Purpose
    Spacer sequence in D1.1 format. Allows for Level 2 cloning with D1.2
    Depositor
    Tom Ellis
    Article
    Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-
    Insert
    Spacer sequence in D1.1 format. Allows for Level 2 cloning with D1.2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    180565-plasmid-map-sequence-id-373326

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Showing: 6561 - 6580 of 6923 results