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Plasmid#108103PurposeLentiviral expression plasmid of human SIK3 cDNA with neomycin resistance geneDepositorInsertSIK3 (SIK3 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEFS promoterAvailable sinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
FRB-CTEVp(WT)
Plasmid#137832PurposeCytosolic split-TEV protease chain with FRB Rapamycin sensing domain, C-terminal WT TEV proteaseDepositorInsertFRB-CTEVp(WT)
UseTags3x FLAG and NESExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBabe Blast osTIR1-9Myc
Plasmid#80073PurposeRetroviral vector for stable expression of osTIR1-9Myc in mammalian cell linesDepositorInsertOsTIR1
UseRetroviralTags9 x MycExpressionMutationPromoterGagAvailable sinceJuly 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_PIK3CA_p.H1047R
Plasmid#82824PurposeGateway Donor vector containing PIK3CA, part of the Target Accelerator Plasmid Collection.DepositorInsertPIK3CA (PIK3CA Human)
UseGateway entry vectorTagsExpressionMutationH1047RPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRRL-MHCK7-GCaMP6
Plasmid#65042PurposeLentiviral vector expressing the calcium indicator GCaMP6 driven by the muscle-specific promoter MHCK7DepositorInsertGCaMP6
UseLentiviralTags6xHis, T7 tag, and Xpress epitopeExpressionMammalianMutationPromoterMHCK7Available sinceJune 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBS31-cCARLIN
Plasmid#160928PurposeContains 10 constitutively expressed guide RNAs and CAG-GFP-CARLIN array sequence for DNA barcoding (when used in combination with Cas9)DepositorInsertCARLIN gRNAs and CAG-GFP-CARLIN array
UseMouse TargetingTagsExpressionMammalianMutationPromoterU6 promoter for gRNAs; CAG promoter for GFPAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-6xGLY-Strep-tagII-hphMX4
Plasmid#20758DepositorTypeEmpty backboneUsePcr-based c-terminal taggingTagsExpressionMutationPromoterAvailable sinceMarch 23, 2009AvailabilityAcademic Institutions and Nonprofits only -
FKBP-mCherry
Plasmid#108122PurposeRecruitable fluorophoreDepositorInsertFKBP1A(isoform a, 3-108):mCherry (FKBP1A Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBiFC-PBZ-VN
Plasmid#110646Purposeexpresses a PAR-binding (PBZ) domain fused to venus N-terminusDepositorInsertPBZ domain from APLF, amino acids 371-451 (APLF Human)
UseTagsDDK and VNExpressionMammalianMutationPromoterCMVAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBiFC-PBZ-VC
Plasmid#110648Purposeexpresses a PAR-binding (PBZ) domain fused to venus C-terminusDepositorInsertPBZ domain from APLF, amino acids 371-451 (APLF Human)
UseTagsHA and VCExpressionMammalianMutationPromoterCMVAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-H2B-GFP
Plasmid#116869PurposeNuclear localized GFPDepositorInsertGFP
UseAAVTagsExpressionMutationPromoterAvailable sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
3xflag-APEX2-LRRK2
Plasmid#164622PurposeExpresses LRRK2 tagged with APEX2 and 3xFLAGDepositorInsertLRRK2 (LRRK2 Human)
UseTags3xFLAG and APEX2ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
NYESO alpha into TRAC HDRT Source (pTR 223)
Plasmid#112023PurposeDNA sequence source for amplifying an HDR template to replace endogenous human TCR-alpha with 1G4 NYESO TCR-alphaDepositorInsertNYESO alpha into TRAC HDRT
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
TFORF2885
Plasmid#144256PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertGLI2 (GLI2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lck-FRB-ECFP(W66A)
Plasmid#67902PurposeExpresses a membrane located FRB domain in mammalian cells (the ECFP is not fluorescent)DepositorInsertLck-FRB-ECFP(W66A)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBABE-puro-hTERT-HA
Plasmid#1772DepositorInserthTERT (TERT Human)
UseRetroviralTags2XHAExpressionMammalianMutationPromoterAvailable sinceJune 3, 2005AvailabilityAcademic Institutions and Nonprofits only -
pTMB352_ZFcharm_Kv1_split-inteins
Plasmid#220848PurposeLentiviral vector expressing ZFcharm Kv2 fused to ZF3 and MmZFP_81187 via split Npu inteins; perfect match ZF3 binding site located upstream of pEFSDepositorInsertZFcharm Kv2
UseLentiviralTagsP2A-mCherryExpressionMammalianMutationN/APromoterEFSAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
GFP Snail WT
Plasmid#16225DepositorInsertSnail (SNAI1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ClopHensor
Plasmid#25938DepositorInsertClopHensor
UseTagsStrep-IIExpressionMammalianMutationE2GFP is EGFP-T203YPromoterAvailable sinceSept. 13, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAAV_BiLAMP5e3_dTomato_nlsdTomato_nlsdTomato
Plasmid#213914PurposeAAV construct expressing dTomato driven by Lamp5 interneuron-targeting enhancer. Alias: pAAV_WDL0003_dTomato_nlsdTomato_nlsdTomatoDepositorInsertdTomato_P2A_nls.dTomato_P2A_nls.dTomato
UseAAVTagsExpressionMutationPromoterAvailable sinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLL39
Plasmid#15458DepositorInsert
UseS. aureus-e. coli shuttle vectorTagsExpressionMutationPromoterAvailable sinceOct. 9, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-rsChRmine-oScarlet-KV 2.1-WPRE
Plasmid#183523PurposeOptogeneticsDepositorInsertrsChRmine-oScarlet-Kv2.1
UseAAVTagsExpressionMutationI146M/G174SPromoterCaMKIIaAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRY2PHR-mCherryN1
Plasmid#26866PurposeExpresses CRY2(1-498)-mCherry fusion for use in light-inducible protein interaction modulesDepositorInsertCRY2PHR (CRY2 Mustard Weed)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
His6_MBP_FXR2P_P474S_P493N
Plasmid#198695PurposeExpression of human FXR2 protein in E. coliDepositorInsertFXR2P (FXR2 Human)
UseTagsMBPExpressionBacterialMutationChanged proline 474 to serine and proline 493 asp…PromoterT7Available sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
GW1-Mito-pHRed
Plasmid#31474DepositorInsertCox8-tagged pHRed
UseTags4xCox8ExpressionMammalianMutationPromoterAvailable sinceJuly 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLenti X1 Puro DEST (694-6)
Plasmid#17297Purpose3rd gen lentiviral promoter-less Gateway destination vector, PuroDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral and RNAi; Destination vectorTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1-Luciferase
Plasmid#99283PurposeAll-in-one lentivector with tet-inducible luciferaseDepositorInsertFirefly luciferase
UseLentiviralTagsExpressionMammalianMutationPromoterTREAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
TFORF3376
Plasmid#144852PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTOX (TOX Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
iCas
Plasmid#84232PurposeExpression of SpCas9 with 4 ERT2 fusion protein and empty gRNA cassette. The activity of Cas9 can be switched on and off in human cells with 4-hydroxytamoxifen (4-HT)DepositorInsertCas9
UseTags2A-OFP co-expression and ERT2-ERT2ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-spCas9 ABE-Umax
Plasmid#222138PurposeAdenine base editor with modified TadA variant for A-to-G base editing in zebrafishDepositorInsertModified TadA-nSpCas9 (cas9 Synthetic)
UseCRISPR; Zebrafish expressionTagsbipartite NLSExpressionMutationPromoterT3Available sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only