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Plasmid#80070PurposeExpresses GFP-tagged BLM helicase in mammalian cells (EGFP-C1 backbone)DepositorInsertBLM (BLM Human)
UseTagsGFPExpressionMammalianMutationIf correct ID, the cDNA is from HeLa and has char…PromoterAvailable sinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFUGW-FerH-ffLuc2-eGFP
Plasmid#71393PurposeLentiviral vector of luciferase-eGFP fusion gene driven by FerH promoterDepositorInsertFerH-ffLuc2-eGFP
UseLentiviralTagsExpressionMammalianMutationPromoterFerHAvailable sinceDec. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO_EGFP-GFE3
Plasmid#79871PurposeFor generating adeno-associated virus to express GFE3 under the EF1a promoter in a 'Cre-on' dependent manner.DepositorInsertGFP-GFE3
UseAAV, Cre/Lox, and Synthetic BiologyTagsGFP and HAExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
MSP680
Plasmid#65772PurposeHuman expression vector for SpCas9 EQR variant: CMV-T7-humanSpCas9(D1135E/R1335Q/T1337R)-NLS-3xFLAG (EQR variant)DepositorInsertmammalian codon-optimized Streptococcus pyogenes Cas9 (D1135E/R1335Q/T1337R)-NLS-3XFlag
UseCRISPRTags3x FLAG and NLSExpressionMammalianMutationD1135E, R1335Q, and T1337R mutations in Cas9PromoterCMV & T7Available sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
GFP-HA-BACE1
Plasmid#165032PurposeHA-BACE1DepositorInsertBACE1 (BACE1 Human)
UseTagsHA (embedded after propeptide cleavage site)ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBS-KS-attB1-2-PT-SA-SD-1-GFP11x7
Plasmid#172067PurposeProtein trap plasmid for inserting GFP11x7 into a phase 1 coding intronDepositorInsertGFP11x7
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBeta-lacZ-attBx2
Plasmid#124445PurposePlasmid for in vivo enhancer reporter assay. Plasmid contains a β-globin minimal promoter followed by a lacZ reporter gene derived from pRS16, with the entire reporter cassette flanked by attB sites.DepositorTypeEmpty backboneUseEnhancer reporter assay constructTagsLacZExpressionMammalianMutationPromoterbeta globin minimal promoterAvailable sinceMay 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG_GPHN.FingR-mKate2-IL2RGTC
Plasmid#46297DepositorInsertGPHN.FingR-mKate2-IL2RGTC (GPHN Synthetic, Human)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-XL4 ASXL1 (1-1304)
Plasmid#74244PurposeCancer-associated ASXL1 truncation mutant in mammalian expression vectorDepositorInsertASXL1 (ASXL1 Human)
UseTagsExpressionMammalianMutationContains ASXL1 amino acids 1-1304PromoterCMVAvailable sinceDec. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGW_PSD95.FingR-YFP
Plasmid#46299DepositorInsertPSD95.FingR (DLG4 Synthetic, Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUBC_stdMCP_serinemod_E488QADAR_p2A_yGFP
Plasmid#154787PurposeExpresses synonymized MS2 coat protein dimer (MCP) fused to hyperactive ADAR catalytic domainDepositorInsertAdenosine deaminse (Adar Fly, Synthetic)
UseLentiviralTagsGFP, V5, and stdMCPExpressionMammalianMutationSynonymized serine to prevent autoediting of ADAR…PromoterUBCAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-AncBE4max
Plasmid#174164PurposeThis is an AncBE4max (a cytosine base editor) expressing plasmid built on the 3rd generation system of lentiviral vector. It can be used for stable expression of AncBE4max in cells.DepositorInsertAncBE4max
UseLentiviralTagsP2A-mCherryExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pssGFP-hCRT
Plasmid#80022PurposeMammalian expression of signal peptide-EGFP-humanCRTDepositorInsertCRT (CALR Human)
UseTagsEGFP and signal peptideExpressionMammalianMutationsignal peptide 17aa added before GFP-CRT, will ta…PromoterCMVAvailable sinceJuly 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
Cav2.2 pcDNA3
Plasmid#206072Purposeexpression of rabbit Cav2.2 calcium channel, the 3'UTR is deleted, this enhances the expressionDepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag Jnk1a1
Plasmid#13798DepositorInsertJnk1a1 (MAPK8 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2007AvailabilityAcademic Institutions and Nonprofits only -
pyc111s
Plasmid#158781Purposeribosome-tagged, Cre-dependent GCaMP for soma localizationDepositorInsertGCaMP6s-RPL10a
UseAAVTagsExpressionMutationPromoterAvailable sinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dLight1.2
Plasmid#111054PurposeExpresses dLight1.2 in mammalian cellsDepositorInsertdLight1.2
UseTagsFlag tagExpressionMammalianMutationPromoterCAGAvailable sinceSept. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLVX-opto-hCaspase-4 dCARD
Plasmid#208785PurposeInducibly expresses human Caspase 4 lacking the CARD domain, fused to light-activatable Cry2DepositorInsertCry2 mCherry Caspase4 dCARD (CASP4 Human)
UseLentiviralTagsCry2 mCherryExpressionMammalianMutationCARD domain is removedPromoterAvailable sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX330 _CRISPIE donor B9
Plasmid#172850PurposeDRS-2 sgRNA expression under a U6 promotor and CRISPIE donor B9 (Zhong et al, eLife 2021), mEGFP translational phase (2-2), excised by DRS-1 or DRS-2 sgRNA (with SpCas9).DepositorInsertDRS-2 sgRNA and CRISPIE designer exon (phase 2-2) encoding mEGFP
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330 _CRISPIE donor B8
Plasmid#172849PurposeDRS-2 sgRNA expression under a U6 promotor and CRISPIE donor B8 (Zhong et al, eLife 2021), mEGFP translational phase (1-1), excised by DRS-1 or DRS-2 sgRNA (with SpCas9).DepositorInsertDRS-2 sgRNA and CRISPIE designer exon (phase 1-1) encoding mEGFP
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only