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6x-His_A1aY1_GFP

Plasmid

#158753

Purpose

Bacterial expression of 6xHis-A1aY1 fused with carboxy terminus GFP for recombinant purification

Depositor

Minhajuddin Sirajuddin

Article

Kesarwani et al J Cell Biol. 2020 Oct 5;219(10). pii: 15

Insert

6xHistidine_A1aY1_GFP

Use

Tags

6x-Histidine tag, GFP, T7 leader sequence, and Th…

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pJL2-mGold-P2A-EBFP2

Plasmid

#157998

Purpose

Co-expression of mGold and EBFP2 in mammalian cells.

Depositor

Francois St-Pierre

Article

Lee et al Sci Adv. 2020 Oct 23;6(43). pii: 6/43/ea

Insert

mGold-P2A-EBFP2

Use

Tags

Expression

Mammalian

Mutation

mGold is mVenus with L46F;T63S mutations

Promoter

CMV

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pRR-ERalpha-5Z

Plasmid

#23061

Depositor

Charles Miller

Article

Miller et al Plasmid. 2009 Dec 3. ():.

Insert

ERalpha (ESR1 Human)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 31, 2010

Availability

Academic Institutions and Nonprofits only
pU6-(BbsI)sgRNA_CAG-Cas9-venus-bpA

Plasmid

#86986

Purpose

For cloning and expression of sgRNA together with expression of a Cas9-Venus fusion protein

Depositor

Ralf Kuehn

Article

Yumlu et al Methods Mol Biol. 2019;1961:137-151. doi

Insert

Cas9-venus

Use

Tags

Cas9-Venus fusion protein

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only
pcDNA-Wnt7B

Plasmid

#35915

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt7B (Wnt7b Human)

Use

Tags

Expression

Mammalian

Mutation

None

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 mSTIM1 myc

Plasmid

#17732

Depositor

Anjana Rao

Article

Oh-Hora et al Nat Immunol. 2008 Apr . 9(4):432-43.

Insert

STIM1 (Stim1 Mouse)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

April 14, 2008

Availability

Academic Institutions and Nonprofits only
pRS426-GFP-Osh2-PH

Plasmid

#71373

Purpose

For analysis of PH domain localization in yeast

Depositor

Mark Lemmon

Article

Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

Insert

Osh2-PH

Use

Tags

EGFP

Expression

Yeast

Mutation

Pleckstrin homology (PH) domain; aa 277-398

Promoter

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only
pHR-PGK-CRY2PHR-VPR

Plasmid

#192608

Purpose

Lentiviral vector for constitutive expression of CRY2PHR-VPR.

Depositor

Yingxiao Wang

Article

Huang et al Sci Adv. 2020 Feb 19;6(8):eaay9209. doi:

Insert

CRY2PHR-VPR

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pRhaQCas-BsaI

Plasmid

#170631

Purpose

Expression of CRISPR-associated transposases

Depositor

Sheng Yang

Article

Zhang et al CRISPR J. 2021 Jun;4(3):350-359. doi: 10

Insert

VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(BsaI)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA-Wnt1-V5

Plasmid

#35924

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt1 (WNT1 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

None

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.nucGAP

Plasmid

#78736

Purpose

Expression of Ca2+ sensor GAP in the nucleus

Depositor

Teresa Alonso

Article

Rodriguez-Garcia et al Proc Natl Acad Sci U S A. 2014 Feb 18;11

Insert

nucGAP

Use

Tags

nucleoplasmin (Xenopus)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 21, 2016

Availability

Academic Institutions and Nonprofits only
pCMV-PDK1-FLAG-LOV*

Plasmid

#202205

Purpose

Expresses the photoproximity labeling catalyst LOV* fused to PDK1 in mammalian cells

Depositor

Thomas Muir

Article

Hananya et al Proc Natl Acad Sci U S A. 2023 Apr 18;12

Insert

[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial (PDK1 Human)

Use

Tags

FLAG and LOV*

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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pSLC-241

Plasmid

#73194

Purpose

Template plasmid which encodes kanamycin resistance gene for positive selection and toxin gene (mqsR) under the control of rhamnose induceable promoter (PrhaB) for negative selection.

Depositor

Swaine Chen

Article

Khetrapal et al Nucleic Acids Res. 2015 Jul 27;43(13):e8

Insert

PrhaB-mqsR (mqsR )

Use

Tags

Expression

Bacterial

Mutation

Promoter

PrhaB

Available since

June 30, 2016

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-Purg1-3HA

Plasmid

#39351

Depositor

Jurg Bahler

Article

Watt et al PLoS One. 2008 Jan 16;3(1):e1428.

Insert

urg1 promoter (urg1, urg1 Fission Yeast)

Use

Yeast genomic targeting

Tags

3xHA and kanMX6 module - reverse orientation

Expression

Mutation

Promoter

Available since

Jan. 10, 2013

Availability

Academic Institutions and Nonprofits only
pRR-AR-5Z

Plasmid

#23058

Depositor

Charles Miller

Article

Miller et al Plasmid. 2009 Dec 3. ():.

Insert

AR (AR Human)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 29, 2010

Availability

Academic Institutions and Nonprofits only
pJRA104

Plasmid

#209337

Purpose

Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal HaloTag fusion protein.

Depositor

Lucia Strader

Article

Allen et al Res Sq. 2023 Nov 7:rs.3.rs-3558280. doi:

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pUC-DTA-DEST-R3R4(R)

Plasmid

#141011

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

DTA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAGM51559

Plasmid

#153226

Purpose

Control plasmid for knockout of TRY and CPC genes

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Insert

Cas9, guide RNA for AP3, FAST marker

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pBT346_pLambda-attB-pCA-GFP-pA

Plasmid

#52551

Purpose

for producing PhiC31 attB-containing minicircle in vitro

Depositor

Liqun Luo

Article

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

Lambda integrase attL1
Lambda integrase attR1
PhiC31o attB (full length)
GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pJRA108

Plasmid

#209341

Purpose

Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal mCherry fusion protein.

Depositor

Lucia Strader

Article

Allen et al Res Sq. 2023 Nov 7:rs.3.rs-3558280. doi:

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge